{
  "abstract": "The COP9 signalosome is composed of 8 subunits (CSN1-CSN8) and plays a key role in regulating the activity of the ubiquitin-proteasome system by modulating Cullin-RING ubiquitin ligases. While the function of the COP9 signalosome in regulating protein homeostasis is well established, little is known about its role in glioblastoma. Here, using patient-derived glioblastoma stem cell lines, we identified CSN1 as a crucial COP9 signalosome subunit for in vitro growth. CSN1 knockout was further found to significantly extend survival in orthotopic glioblastoma xenograft models. Transcriptomic analysis revealed that CSN1 knockout disrupted transcriptional programs involved in cell cycle progression and activated apoptotic pathways. Molecular analysis further determined that CSN1 is required for COP9 signalosome complex assembly and regulates AP-1 activation via JNK1/2/3 and c-Jun phosphorylation. These results highlight the essentiality of CSN1 for glioblastoma stem cell growth and provide insights into the cellular mechanisms controlled by CSN1 and the COP9 signalosome.",
  "abstract_shown": true,
  "affiliations": [
    "Arnie Charbonneau Cancer Institute, Cumming School of Medicine, University of Calgary, Calgary, Alberta, Canada",
    "Hotchkiss Brain Institute, Cumming School of Medicine, University of Calgary, Calgary, Alberta, Canada",
    "Department of Cell Biology and Anatomy, University of Calgary, Calgary, Alberta, Canada"
  ],
  "article_type": "Research Article",
  "arxiv": null,
  "author_total": 5,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Samir Assaf"
    },
    {
      "affs": [
        1
      ],
      "name": "Kyle Heemskerk"
    },
    {
      "affs": [
        2
      ],
      "name": "Xiaoguang Hao"
    },
    {
      "name": "H. Artee Luchman"
    },
    {
      "name": "Samuel Weiss"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/e4vavny4caze9.bib",
  "cited_by": 0,
  "doi": "10.1371/journal.pone.0358568",
  "fulltext_url": null,
  "id": "e4vavny4caze9",
  "issns": [
    "1932-6203"
  ],
  "landing_url": "https://doi.org/10.1371/journal.pone.0358568",
  "language": null,
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "journal",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "2716|Glioma Diagnosis and Treatment"
    ],
    [
      "plos",
      "/Biology and life sciences/Biochemistry/Proteins/Post-translational modification/Phosphorylation"
    ],
    [
      "plos",
      "/Biology and life sciences/Biochemistry/Proteins/Protein complexes/Signalosomes"
    ],
    [
      "plos",
      "/Biology and life sciences/Cell biology/Cell processes/Cell cycle and cell division"
    ],
    [
      "plos",
      "/Biology and life sciences/Cell biology/Signal transduction/Cell signaling/STAT signaling"
    ],
    [
      "plos",
      "/Biology and life sciences/Genetics/Gene expression/Gene regulation/Transcriptional control"
    ],
    [
      "plos",
      "/Medicine and health sciences/Gastroenterology and hepatology/Liver diseases/Hepatocellular carcinoma"
    ],
    [
      "plos",
      "/Medicine and health sciences/Neurology/Neurological tumors/Glioblastoma multiforme"
    ],
    [
      "plos",
      "/Medicine and health sciences/Oncology/Cancers and neoplasms/Blastoma/Glioblastoma multiforme"
    ],
    [
      "plos",
      "/Medicine and health sciences/Oncology/Cancers and neoplasms/Carcinoma/Hepatocellular carcinoma"
    ],
    [
      "plos",
      "/Medicine and health sciences/Oncology/Cancers and neoplasms/Gastrointestinal tumors/Hepatocellular carcinoma"
    ],
    [
      "plos",
      "/Medicine and health sciences/Oncology/Cancers and neoplasms/Neurological tumors/Glioblastoma multiforme"
    ],
    [
      "plos",
      "/Research and analysis methods/Spectrum analysis techniques/Spectrophotometry/Cytophotometry/Flow cytometry"
    ]
  ],
  "openalex": "W7213465115",
  "pdf_url": "https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0358568&type=printable",
  "pmcid": null,
  "pmid": null,
  "published": "2026-09-17",
  "publisher": "Public Library of Science",
  "ris": "https://research.draconitic.com/paper/e4vavny4caze9.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:17:49Z",
      "native_id": "10.1371/journal.pone.0358568",
      "source": "plos",
      "url": "https://doi.org/10.1371/journal.pone.0358568"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "plos:cell biology",
      "subfield": "cell-biology"
    },
    {
      "field": "medicine",
      "origin": "plos:cancers and neoplasms",
      "subfield": "oncology"
    }
  ],
  "title": "The COP9 signalosome subunit CSN1 regulates glioblastoma stem cell growth",
  "title_text": "The COP9 signalosome subunit CSN1 regulates glioblastoma stem cell growth",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:17:49Z",
  "url": "https://research.draconitic.com/paper/e4vavny4caze9",
  "venue": "PLOS One",
  "versions": []
}