{
  "abstract": "In situ subtomogram averaging (STA) for high-resolution structure determination requires fine pixel sampling, forcing a trade-off between achievable resolution and the field of view (FOV) recorded in each tomogram. Electron-event representation (EER) data can be rendered at half the physical pixel size, but whether super-resolution processing can recover useful information beyond the physical Nyquist limit from noisy in situ data remains unclear. Here, we show that super-resolution processing of EER data recovers biologically interpretable information beyond a physical Nyquist limit of 4.78 A in two in situ datasets. STA of unidentified virus particles in a Drosophila melanogaster larva yielded a 3.42 A inner capsid reconstruction, enabling de novo model building and identification of the particles as Drosophila A virus. In situ STA of ribosomes in Saccharomyces cerevisiae reached 3.18 A, with local resolution extending to 2.78 A. EER acquisition therefore provides greater flexibility in choosing the acquisition magnification: existing datasets can be re-rendered at the super-resolution pixel size when physical sampling becomes limiting, while lower-magnification acquisition can be considered when a larger FOV improves target capture or cellular context.",
  "abstract_shown": true,
  "affiliations": [
    "EMBL"
  ],
  "article_type": "New results",
  "arxiv": null,
  "author_total": 4,
  "authors": [
    {
      "name": "E. Pyle"
    },
    {
      "name": "S. Bekbayev"
    },
    {
      "name": "V. Dmello"
    },
    {
      "affs": [
        0
      ],
      "name": "S. Mattei"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/e546dj60m6697.bib",
  "cited_by": 0,
  "doi": "10.64898/2026.10.17.759810",
  "fulltext_url": "https://www.biorxiv.org/content/10.64898/2026.10.17.759810v1.full",
  "id": "e546dj60m6697",
  "issns": [],
  "landing_url": "https://www.biorxiv.org/content/10.64898/2026.10.17.759810v1",
  "language": null,
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "biorxiv",
      "molecular biology"
    ],
    [
      "openalex",
      "1315|Advanced Electron Microscopy Techniques and Applications"
    ]
  ],
  "openalex": "W7222959642",
  "pdf_url": "https://www.biorxiv.org/content/10.64898/2026.10.17.759810v1.full.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-17",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/e546dj60m6697.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:28:36Z",
      "native_id": "10.64898/2026.10.17.759810",
      "source": "biorxiv",
      "url": "https://www.biorxiv.org/content/10.64898/2026.10.17.759810v1"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "biorxiv:molecular biology",
      "subfield": "molecular-biology"
    }
  ],
  "title": "Super-Resolution Processing Enables In Situ Subtomogram Averaging Beyond the Nyquist Limit",
  "title_text": "Super-Resolution Processing Enables In Situ Subtomogram Averaging Beyond the Nyquist Limit",
  "type": "preprint",
  "updated_at": "2026-10-30T12:28:36Z",
  "url": "https://research.draconitic.com/paper/e546dj60m6697",
  "venue": "bioRxiv",
  "versions": []
}