{
  "abstract": "Diploid genomes carry millions of heterozygous variants in cis-regulatory DNA, yet most genes produce similar RNA output from two parental alleles. How this balance is maintained is unclear. We developed Nanopore-HiChIP, a long-read method that maps high-order enhancer hubs on each haplotype. Over half of these enhancer hubs differ in chromatin architecture and transcription-factor occupancy between homologous chromosomes, but their target genes show substantially lower rates of allele-specific expression than genes lacking hub regulation. Single-cell kinetic modeling shows that burst frequency and burst size change in opposite directions, thereby preserving balanced transcriptional output. This hub-mediated kinetic buffering is enriched at haploinsufficient genes and coincides with smaller effects of expression quantitative trait loci. Enhancer hubs therefore absorb allelic regulatory variation through kinetic compensation, protecting dosage-sensitive transcription.",
  "abstract_shown": true,
  "affiliations": [
    "Washington University in St. Louis"
  ],
  "article_type": "New results",
  "arxiv": null,
  "author_total": 11,
  "authors": [
    {
      "name": "J. Tan"
    },
    {
      "name": "M. Sentmanat"
    },
    {
      "name": "Y. Wu"
    },
    {
      "name": "C. Peng"
    },
    {
      "name": "C. Fronick"
    },
    {
      "name": "C. Markovic"
    },
    {
      "name": "X. Cui"
    },
    {
      "name": "R. Fulton"
    },
    {
      "name": "R. Head"
    },
    {
      "name": "T. Wang"
    },
    {
      "affs": [
        0
      ],
      "name": "Y. Sun"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/e7gagcsabg5mg.bib",
  "cited_by": 0,
  "doi": "10.64898/2026.09.14.750771",
  "fulltext_url": "https://www.biorxiv.org/content/10.64898/2026.09.14.750771v1.full",
  "id": "e7gagcsabg5mg",
  "issns": [],
  "landing_url": "https://www.biorxiv.org/content/10.64898/2026.09.14.750771v1",
  "language": null,
  "licence": "CC-BY-NC",
  "licence_label": "CC BY-NC",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "biorxiv",
      "genomics"
    ],
    [
      "openalex",
      "1312|Genomics and Chromatin Dynamics"
    ]
  ],
  "openalex": "W7213871725",
  "pdf_url": "https://www.biorxiv.org/content/10.64898/2026.09.14.750771v1.full.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-09-14",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/e7gagcsabg5mg.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:23:27Z",
      "native_id": "10.64898/2026.09.14.750771",
      "source": "biorxiv",
      "url": "https://www.biorxiv.org/content/10.64898/2026.09.14.750771v1"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "biorxiv:genomics",
      "subfield": "genetics-genomics"
    }
  ],
  "title": "High-order enhancer hubs buffer allelic regulatory variation through kinetic compensation",
  "title_text": "High-order enhancer hubs buffer allelic regulatory variation through kinetic compensation",
  "type": "preprint",
  "updated_at": "2026-10-30T12:23:27Z",
  "url": "https://research.draconitic.com/paper/e7gagcsabg5mg",
  "venue": "bioRxiv",
  "versions": []
}