{
  "abstract": "The binding affinities of a chimeric analog of a riboflavin derivative linked to biotin, (6- (7,8-dimethyl-2,4-dioxo-3,4-dihydrobenzo[g]pteridin-10(2H)-yl)hexyl 5-((3aS,4S,6aR)-2- oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanoate), referred to as C6-Rf-biotin-tag, to the riboflavin binding retain or streptavidin are in the M range, 1.29 {+/-} 0.277 and 3.00 {+/-} 0.459, respectively. These values suggest that C6-Rf-biotin-tag has potential applications in diagnostic assay and labelling target flavin binding proteins. The C6-Rf-biotin-tag which was characterized with respect to physical and biochemical properties retains UV/Vis spectroscopic and fluorescence behavior similar to riboflavin.",
  "abstract_shown": true,
  "affiliations": [
    "University of Michigan-Dearborn"
  ],
  "article_type": "New results",
  "arxiv": null,
  "author_total": 5,
  "authors": [
    {
      "name": "S. Marincean"
    },
    {
      "name": "S. R. Smith"
    },
    {
      "name": "T. Branscum"
    },
    {
      "name": "A. Ratajczak"
    },
    {
      "affs": [
        0
      ],
      "name": "M. A. Benore"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/e7j35cvn54r47.bib",
  "cited_by": 0,
  "doi": "10.64898/2026.08.29.748002",
  "fulltext_url": "https://www.biorxiv.org/content/10.64898/2026.08.29.748002v1.full",
  "id": "e7j35cvn54r47",
  "issns": [],
  "landing_url": "https://www.biorxiv.org/content/10.64898/2026.08.29.748002v1",
  "language": null,
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "biorxiv",
      "biochemistry"
    ],
    [
      "openalex",
      "1312|Protein Interaction Studies and Fluorescence Analysis"
    ]
  ],
  "openalex": "W7204755985",
  "pdf_url": "https://www.biorxiv.org/content/10.64898/2026.08.29.748002v1.full.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-08-29",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/e7j35cvn54r47.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:17:35Z",
      "native_id": "10.64898/2026.08.29.748002",
      "source": "biorxiv",
      "url": "https://www.biorxiv.org/content/10.64898/2026.08.29.748002v1"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|Protein Interaction Studies and Fluorescence Analysis",
      "subfield": "molecular-biology"
    }
  ],
  "title": "Biochemical and Binding Characterization of a Riboflavin Analogue Tethered to Biotin",
  "title_text": "Biochemical and Binding Characterization of a Riboflavin Analogue Tethered to Biotin",
  "type": "preprint",
  "updated_at": "2026-10-30T12:17:35Z",
  "url": "https://research.draconitic.com/paper/e7j35cvn54r47",
  "venue": "bioRxiv",
  "versions": []
}