{
  "abstract": "This study establishes a rapid detection method for Yersinia pestis that combines recombinase-aided amplification with a lateral flow dipstick (RAA-LFD) and targets the pla gene. Based on the pla gene sequences of six representative strains from NCBI, three primer pairs (P-F1/R1, P-F2/R2, and P-F3/R3) and one RAA-nfo probe were designed. The RAA-LFD assay was constructed and evaluated for sensitivity, specificity, repeatability, and clinical applicability. Initial screening via polymerase chain reaction followed by agarose gel electrophoresis (PCR-AGE) showed P-F3/R3 produced a faint band, while P-F1/R1 and P-F2/R2 yielded bright, specific bands. RAA-LFD condition optimization demonstrated that: clear test lines (T lines) were observable at primer concentrations of 250-2000 nM, with the strongest signal at 2000 nM; the T line signal of P-F1/R1 remained stable at 35-41 °C, peaking slightly at 39 °C, whereas P-F2/R2 decreased markedly at 35 °C (p < 0.01); and the integrated optical density (IOD) of the T line increased with reaction time from 5 to 30 min, with a significant difference between 5 and 30 min (p < 0.001). The optimal reaction conditions were consequently determined as follows: primer pair P-F1/R1, a final primer concentration of 2000 nM, and incubation at 39 °C for 30 min. All five Y. pestis strains tested positive, while seven non-target strains and blank controls were negative, consistent with PCR results. Regarding sensitivity, the limit of detection (LOD) of RAA-LFD was 3.8 × 10² copies/μL, approximately 10-fold lower than conventional PCR (3.8 × 10³ copies/μL). In the repeatability evaluation, intra-assay CVs were 1.79%, 0.59%, and 7.76%; inter-assay CVs were 1.75%, 2.35%, and 1.70%, all within 10%. Results for 130 clinical samples showed 100% agreement with PCR and qPCR. RAA-LFD established in this study provides a reliable, simple approach for on-site plague screening and primary laboratory diagnosis.",
  "abstract_shown": true,
  "affiliations": [
    "Key Laboratory of Animal Disease and Human Health of Sichuan, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China.",
    "College of Veterinary Medicine, Northwest A&F University, Yangling 712100, China."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 10,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Yunhan Zhou"
    },
    {
      "affs": [
        0
      ],
      "name": "Xue Yang",
      "orcid": "0009-0009-9994-3060"
    },
    {
      "affs": [
        0
      ],
      "name": "Ziling Ding"
    },
    {
      "affs": [
        0
      ],
      "name": "Qiuyu Fan"
    },
    {
      "affs": [
        0
      ],
      "name": "Xiaofang Yan"
    },
    {
      "affs": [
        0
      ],
      "name": "Haifeng Liu",
      "orcid": "0000-0002-7495-9332"
    },
    {
      "affs": [
        0
      ],
      "name": "Ziyao Zhou"
    },
    {
      "affs": [
        0
      ],
      "name": "Zhijun Zhong"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Jialiang Xin"
    },
    {
      "affs": [
        0
      ],
      "name": "Guangneng Peng",
      "orcid": "0000-0002-9898-5754"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/e8c71215haght.bib",
  "cited_by": 0,
  "doi": "10.3390/vetsci13101091",
  "fulltext_url": "https://europepmc.org/articles/PMC13709453",
  "id": "e8c71215haght",
  "issns": [
    "2306-7381"
  ],
  "landing_url": "https://europepmc.org/article/MED/42944185",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "2204|Biosensors and Analytical Detection"
    ]
  ],
  "openalex": "W7221813824",
  "pdf_url": "https://europepmc.org/articles/PMC13709453?pdf=render",
  "pmcid": "PMC13709453",
  "pmid": "42944185",
  "published": "2026-10-14",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/e8c71215haght.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:22:12Z",
      "native_id": "MED:42944185",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42944185"
    }
  ],
  "subjects": [],
  "title": "Establishment of Recombinase-Aided Amplification Combined with Lateral Flow Dipstick for Rapid Detection of Yersinia pestis Based on the pla Gene.",
  "title_text": "Establishment of Recombinase-Aided Amplification Combined with Lateral Flow Dipstick for Rapid Detection of Yersinia pestis Based on the pla Gene.",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:22:12Z",
  "url": "https://research.draconitic.com/paper/e8c71215haght",
  "venue": "Veterinary sciences",
  "versions": []
}