{
  "abstract": "Purpose: Recent studies suggest distinct patterns of DNA methyltransferase (DNMT) expression across different stages of age-related macular degeneration (AMD). However, whether these variations can be modulated by conventional therapeutic approaches remains unexplored. This study investigates DNMT expression dynamics in two patients with exudative neovascular AMD (nAMD) during the initial months of anti-VEGF treatment.\n\nObservations: Case 1, a female in her 90s was seen for sudden loss of vision secondary to macular hemorrhage. During type 1 macular neovascularization (t1MNV) anti-VEGF treatment, DNMT analysis revealed sustained upregulation of DNMT1 (+116%) and DNMT3b (+179%) while exudative MNV remained presisted.\n\nCase 2, a female in her 70s and asymptomatic subretinal fluid secondary to t1MNV showed a distinct DNMT expression pattern, with a more modest increase in DNMT1 (+28.9%) and DNMT3b (+17.6%) when exudation was resolved.\n\nConclusions: We hereby report varying DNMTs expression in nAMD patients with different disease manifestations giving new insights into the dynamic nature of epigenetic modifications in response to anti-VEGF treatment.",
  "abstract_shown": true,
  "affiliations": [
    "H&TRC- Health & Technology Research Center, ESSL- Escola Superior de Saúde de Lisboa, Universidade Politécnica de Lisboa, Portugal",
    "iNOVA4Health, NOVA Medical School, Faculdade de Ciências Médicas, NMS, FCM, Universidade NOVA de Lisboa, Lisboa, Portugal",
    "Instituto de Retina de Lisboa, Lisbon, Portugal",
    "Hospital Beatriz Ângelo, Lisbon, 2674-514, Portugal",
    "Ophthalmology Department, Centro Hospitalar Universitário de Lisboa Central, Lisbon, Portugal",
    "ITI / LARSyS, Instituto Superior Técnico, Universidade de Lisboa, Portugal",
    "Unidade Local de Saúde Almada Seixal, Almada, Portugal"
  ],
  "article_type": "case report",
  "arxiv": null,
  "author_total": 12,
  "authors": [
    {
      "affs": [
        0,
        1
      ],
      "name": "Pedro Camacho"
    },
    {
      "affs": [
        0
      ],
      "name": "Edna Ribeiro"
    },
    {
      "affs": [
        0,
        2,
        1
      ],
      "name": "Bruno Pereira"
    },
    {
      "affs": [
        3,
        2
      ],
      "name": "João Nascimento"
    },
    {
      "affs": [
        2
      ],
      "name": "Paulo Caldeira Rosa"
    },
    {
      "affs": [
        2
      ],
      "name": "José Henriques"
    },
    {
      "affs": [
        4
      ],
      "name": "Sandra Barrão"
    },
    {
      "affs": [
        5
      ],
      "name": "João Afonso"
    },
    {
      "affs": [
        0
      ],
      "name": "Mariana Delgadinho"
    },
    {
      "affs": [
        0
      ],
      "name": "Catarina Ginete"
    },
    {
      "affs": [
        0
      ],
      "name": "Miguel Brito"
    },
    {
      "affs": [
        2,
        6
      ],
      "name": "Diogo Cabral"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/e8t9mw4rxbw7z.bib",
  "cited_by": 0,
  "doi": "10.1016/j.ajoc.2026.102681",
  "fulltext_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13697319/",
  "id": "e8t9mw4rxbw7z",
  "issns": [
    "2451-9936"
  ],
  "landing_url": "https://doi.org/10.1016/j.ajoc.2026.102681",
  "language": "en",
  "licence": "CC-BY-NC-ND-4.0",
  "licence_label": "CC BY-NC-ND 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Case Report"
    ],
    [
      "openalex",
      "2731|Retinal Diseases and Treatments"
    ]
  ],
  "openalex": "W7222452200",
  "pdf_url": "https://europepmc.org/articles/PMC13697319?pdf=render",
  "pmcid": "PMC13697319",
  "pmid": "42935887",
  "published": "2026-10-16",
  "publisher": "Elsevier",
  "ris": "https://research.draconitic.com/paper/e8t9mw4rxbw7z.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:18:23Z",
      "native_id": "PMC13697319",
      "source": "pmc",
      "url": "https://doi.org/10.1016/j.ajoc.2026.102681"
    },
    {
      "first_seen": "2026-10-30T12:22:12Z",
      "native_id": "MED:42935887",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42935887"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2731|Retinal Diseases and Treatments",
      "subfield": "general"
    }
  ],
  "title": "Changes in expression of DNA-methyltransferase in neovascular age-related macular degeneration: Anti-VEGF response or disease severity?",
  "title_text": "Changes in expression of DNA-methyltransferase in neovascular age-related macular degeneration: Anti-VEGF response or disease severity?",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:34:22Z",
  "url": "https://research.draconitic.com/paper/e8t9mw4rxbw7z",
  "venue": "American Journal of Ophthalmology Case Reports",
  "versions": []
}