{
  "abstract": "The photosynthetic flagellate Euglena gracilis (Euglena) exhibits a remarkable metabolic flexibility, enabling the dynamic coordination of energy production and resource partitioning during anaerobic adaptation. While the wax ester synthesis under hypoxia is well-documented, the regulatory interplay between protein abundance and post-translational modifications (PTMs) remains unexplored. A definitive understanding of this metabolic remodeling is hindered by the lack of high-depth proteomic resources and the fragmented genome annotations, which fail to capture Euglena's proteomic diversity and plasticity. Here, we provide a comprehensive Euglena proteome landscape through advanced LC-MS/MS analysis. By leveraging the recently refined proteome annotation, we identified 9,122 protein groups, 12,027 phospho-peptides, and 3,346 ubiquitinated peptides, establishing a comprehensive Euglena's proteome reference. Our results demonstrate that anaerobic adaptation is characterized by distinct phosphorylation and ubiquitination signatures that often occur independently of changes in global protein abundance, revealing a layer of PTM-mediated control over central metabolism during anaerobic adaptation in Euglena. This study provides a foundational resource that bridges the gap between genomic potential and functional metabolic in Euglena. By resolving the interplay between protein levels and PTM-driven regulation, our work offers a new resource for investigating environmental adaptation and provides a rigorous framework for future metabolic engineering in Euglena.",
  "abstract_shown": true,
  "affiliations": [
    "Bioproductivity Informatics Research Team, RIKEN Center for Sustainable Resource Science, Yokohama, Japan.",
    "Kihara Institute for Biological Research, Yokohama City University, Yokohama, Japan.",
    "Department of Biological Science, Faculty of Science and Engineering, Yasuda Women's University, Hiroshima, Japan.",
    "Division of Cell Signaling, Institute of Advanced Medical Sciences, Tokushima University, Tokushima, Japan.",
    "Institute of Plant Science and Resources, Okayama University, Kurashiki, Japan.",
    "Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Jatinangor, West Java, Indonesia.",
    "Research Center for Molecular Biotechnology and Bioinformatics, Universitas Padjadjaran, Bandung, West Java, Indonesia.",
    "RIKEN Baton Zone Program, RIKEN Cluster for Science, Technology and Innovative Hub, Yokohama, Japan.",
    "School of Information and Data Sciences, Nagasaki University, Nagasaki, Japan."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 10,
  "authors": [
    {
      "affs": [
        0,
        1
      ],
      "name": "Yosua",
      "orcid": "0009-0004-8763-9399"
    },
    {
      "affs": [
        0,
        2
      ],
      "name": "Shun Tamaki"
    },
    {
      "affs": [
        3
      ],
      "name": "Kohei Nishino"
    },
    {
      "affs": [
        0
      ],
      "name": "Akihiro Ezoe",
      "orcid": "0000-0002-5515-343X"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Anzu Minami"
    },
    {
      "affs": [
        0,
        4
      ],
      "name": "June-Sik Kim",
      "orcid": "0000-0002-4703-609X"
    },
    {
      "affs": [
        0,
        5,
        6
      ],
      "name": "Ade Rizqi Ridwan Firdaus"
    },
    {
      "affs": [
        0,
        5,
        6
      ],
      "name": "Toto Subroto"
    },
    {
      "affs": [
        3
      ],
      "name": "Hidetaka Kosako"
    },
    {
      "affs": [
        0,
        1,
        7,
        8
      ],
      "name": "Keiichi Mochida"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/eadhknvjvt4jc.bib",
  "cited_by": 0,
  "doi": "10.1080/15592324.2026.2720310",
  "fulltext_url": "https://europepmc.org/articles/PMC13660438",
  "id": "eadhknvjvt4jc",
  "issns": [
    "1559-2316",
    "1559-2324"
  ],
  "landing_url": "https://europepmc.org/article/MED/42850892",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1607|Advanced Proteomics Techniques and Applications"
    ]
  ],
  "openalex": "W7221176879",
  "pdf_url": "https://europepmc.org/articles/PMC13660438?pdf=render",
  "pmcid": "PMC13660438",
  "pmid": "42850892",
  "published": "2026-10-08",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/eadhknvjvt4jc.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:18:02Z",
      "native_id": "MED:42850892",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42850892"
    }
  ],
  "subjects": [
    {
      "field": "chemistry",
      "origin": "openalex:1607|Advanced Proteomics Techniques and Applications",
      "subfield": "physical"
    }
  ],
  "title": "Mapping the proteome landscape in Euglena gracilis: global, phospho, and ubiquitin proteomes under anaerobic response.",
  "title_text": "Mapping the proteome landscape in Euglena gracilis: global, phospho, and ubiquitin proteomes under anaerobic response.",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:18:02Z",
  "url": "https://research.draconitic.com/paper/eadhknvjvt4jc",
  "venue": "Plant signaling & behavior",
  "versions": []
}