{
  "abstract": "BACKGROUND: Herpes simplex virus type 2 (HSV-2) is a major cofactor of human immunodeficiency virus type 1 (HIV-1) sexual acquisition and transmission. In the present study, we investigated whether HIV-1 and HSV-2 may interact at the cellular level by forming HIV-1 hybrid virions pseudotyped with HSV-2 envelope glycoproteins, as was previously reported for HSV type 1. METHODS: We evaluated in vitro the production of HSV-2/HIV-1 pseudotypes in mononuclear CEM cells and epithelial HT29 and P4P cells. We analyzed the incorporation into the HIV-1 membrane of HSV-2 gB and gD, two major HSV-2 glycoproteins required for HSV-2 fusion with the cell membrane, in co-infected cells and in HIV-1-infected P4P cells transfected by plasmids coding for gB or gD. RESULTS: We show that HSV-2 and HIV-1 co-replicated in dually infected cells, and gB and gD were co-localized with gp160. However, HIV-1 particles, produced in HIV-1-infected cells expressing gB or gD after transfection or HSV-2 superinfection, did not incorporate either gB or gD in the viral membrane, and did not have the capacity to infect cells normally non-permissive for HIV-1, such as epithelial cells. CONCLUSION: Our results do not support the hypothesis of HSV-2/HIV-1 pseudotype formation and involvement in the synergistic genital interactions between HIV-1 and HSV-2.",
  "abstract_shown": true,
  "affiliations": [],
  "article_type": null,
  "arxiv": null,
  "author_total": 8,
  "authors": [
    {
      "name": "Jérôme Legoff"
    },
    {
      "name": "Hicham Bouhlal"
    },
    {
      "name": "Maxime Lecerf"
    },
    {
      "name": "Christophe Klein"
    },
    {
      "name": "Hakim Hocini"
    },
    {
      "name": "Ali Si-Mohamed"
    },
    {
      "name": "Martin Muggeridge"
    },
    {
      "name": "Laurent Bélec"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/ebkjmtbt6c8dj.bib",
  "cited_by": 11,
  "doi": "10.1186/1743-422x-4-2",
  "fulltext_url": null,
  "id": "ebkjmtbt6c8dj",
  "issns": [
    "1743-422X"
  ],
  "landing_url": "https://hal.science/hal-05794230v1",
  "language": "en",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "journal",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "hal",
      "0.sdv"
    ],
    [
      "openalex",
      "2713|Herpesvirus Infections and Treatments"
    ]
  ],
  "openalex": "W2140807966",
  "pdf_url": "https://virologyj.biomedcentral.com/counter/pdf/10.1186/1743-422X-4-2",
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-12",
  "publisher": "BioMed Central",
  "ris": "https://research.draconitic.com/paper/ebkjmtbt6c8dj.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:17:54Z",
      "native_id": "hal-05794230",
      "source": "hal",
      "url": "https://hal.science/hal-05794230v1"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2713|Herpesvirus Infections and Treatments",
      "subfield": "epidemiology-public-health"
    }
  ],
  "title": "HSV-2- and HIV-1- permissive cell lines co-infected by HSV-2 and HIV-1 co-replicate HSV-2 and HIV-1 without production of HSV-2/HIV-1 pseudotype particles",
  "title_text": "HSV-2- and HIV-1- permissive cell lines co-infected by HSV-2 and HIV-1 co-replicate HSV-2 and HIV-1 without production of HSV-2/HIV-1 pseudotype particles",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:17:54Z",
  "url": "https://research.draconitic.com/paper/ebkjmtbt6c8dj",
  "venue": "Virology Journal",
  "versions": []
}