{
  "abstract": "Substantial donor-to-donor heterogeneity shapes red blood cell (RBC) storage biology, but the extent to which integrated donor and molecular measurements capture hemolysis and extracellular-vesicle (EV) phenotypes remains incompletely defined. We analyzed the tail-enriched REDS-III RBC-Omics recalled cohort using one storage-day-42 record from 649 donors. Demographic, complete blood count/ferritin, trace-element, metabolomic, lipidomic, and proteomic predictor blocks were benchmarked for three hemolysis and five EV outcomes. The molecular predictors and most outcomes were measured concurrently at day 42; change in total EVs was day 42 minus day 10. Ridge models used 10 repeats of five-fold outer cross-validation with five-fold inner tuning and fully fold-contained preprocessing and feature screening. Mean Pearson correlations were highest for osmotic hemolysis (r=0.63; full model), oxidative hemolysis (r=0.56; full model), RBC-derived CD235a+CD108- EVs (r=0.47; metabolomics), storage hemolysis (r=0.43; full model), and platelet-derived CD41a+CD62p- EVs (r=0.47; metabolomics). Change in total EVs and RBC-derived CD235a+CD108+ EVs were more modest (r=0.27 and 0.26, respectively), whereas total EV counts were not predicted above chance. Max-block permutation testing supported signal for seven outcomes (Benjamini-Hochberg q=0.006) but not total EVs (q=0.726). Elastic-net sensitivity analysis reproduced the principal ranking. Thus, day-42 storage phenotypes contain endpoint-specific distributed molecular signatures. These internally validated, cross-sectional results from a phenotype-enriched cohort do not establish prospective forecasting or transportability to routine donor populations; external validation is required.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Biochemistry and Molecular Genetics, University of Colorado Denver – Anschutz Medical Campus, Aurora, CO, USA",
    "Omix Technologies Inc, Aurora, CO, USA",
    "Vitalant Research Institute, San Francisco CA, USA",
    "Department of Laboratory Medicine, University of California San Francisco, CA, USA",
    "RTI International, Pittsburgh, PA, USA",
    "University of British Columbia, Vancouver, British Columbia, Canada."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 11,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Monika Dzieciatkowska"
    },
    {
      "affs": [
        0
      ],
      "name": "Daniel Stephenson"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Travis Nemkov"
    },
    {
      "affs": [
        2,
        3
      ],
      "name": "Erika G. Marques de Menezes"
    },
    {
      "affs": [
        2,
        3
      ],
      "name": "Mars Stone"
    },
    {
      "affs": [
        4
      ],
      "name": "Gregory R. Keele"
    },
    {
      "affs": [
        4
      ],
      "name": "Grier P. Page"
    },
    {
      "affs": [
        5
      ],
      "name": "Steve Kleinman"
    },
    {
      "affs": [
        2,
        3
      ],
      "name": "Michael P. Busch"
    },
    {
      "affs": [
        2,
        3
      ],
      "name": "Philip J. Norris"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Angelo D’Alessandro"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/ebyzgvr54frez.bib",
  "cited_by": 0,
  "doi": "10.1016/j.brci.2026.100142",
  "fulltext_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13730207/",
  "id": "ebyzgvr54frez",
  "issns": [
    "3050-5984"
  ],
  "landing_url": "https://doi.org/10.1016/j.brci.2026.100142",
  "language": "en",
  "licence": "CC-BY-NC-ND",
  "licence_label": "CC BY-NC-ND",
  "licence_source": "journal",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Article"
    ],
    [
      "openalex",
      "2720|Blood transfusion and management"
    ]
  ],
  "openalex": "W7220718606",
  "pdf_url": "https://www.sciencedirect.com/science/article/pii/S3050598426000971/pdf",
  "pmcid": "PMC13730207",
  "pmid": "42979273",
  "published": "2026-10-06",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/ebyzgvr54frez.ris",
  "sources": [
    {
      "first_seen": "2026-11-10T12:20:56Z",
      "native_id": "PMC13730207",
      "source": "pmc",
      "url": "https://doi.org/10.1016/j.brci.2026.100142"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2720|Blood transfusion and management",
      "subfield": "general"
    }
  ],
  "title": "Multiomics signatures of hemolysis and vesiculation in stored red blood cells",
  "title_text": "Multiomics signatures of hemolysis and vesiculation in stored red blood cells",
  "type": "peer-reviewed",
  "updated_at": "2026-11-10T12:20:56Z",
  "url": "https://research.draconitic.com/paper/ebyzgvr54frez",
  "venue": "Blood red cells & iron",
  "versions": []
}