{
  "abstract": "Native PAGE separation of digitonin-solubilized thylakoid stroma lamellae resolves PSI and cytochrome b6f into separate bands. Mass spectrometric analysis of the 1D native gel bands showed that the bands are baskets of co-migrating complexes along the molecular size axis. Hierarchical clustering confirmed the separation of dimeric cytochrome b6f from PSI in 1D LN-PAGE. Interval-zone free-flow electrophoresis separated the same extracts by charge in a flowing buffer film, and the continuous fractionation indicated co-migration of complexes without the in-gel structural hindrances. A second-dimension LN-PAGE separated the complexes co-migrating in iZE into complexes with defined molecular size, allowing in-gel mass spectrometry identification in the gel bands. Both genotypes showed a 720 kDa fluorescent band in which cytochrome f (PetA) and cytochrome b6 (PetB) join the PSI-LHCI node (stn7, d = 0.50; pph1, d = 0.34). Lhcb1 and Lhcb2 are absent from the 720 kDa node. The 753 kDa band is specific to pph1 (d = 0.38). Phosphorylated LHCII1,2 joins PSI-LHCI there, and PetB is detected in every slice of that band without becoming a leaf of the tree.",
  "abstract_shown": true,
  "affiliations": [
    "University of Stavanger"
  ],
  "article_type": "New results",
  "arxiv": null,
  "author_total": 6,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "L. A. Eichacker"
    },
    {
      "name": "J. Arnold"
    },
    {
      "name": "G. Fucile"
    },
    {
      "name": "A. K. Vatland"
    },
    {
      "name": "K. Stensen"
    },
    {
      "name": "G. Weber"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/efmbajvxtr0pk.bib",
  "cited_by": 0,
  "doi": "10.64898/2026.10.16.759642",
  "fulltext_url": "https://www.biorxiv.org/content/10.64898/2026.10.16.759642v1.full",
  "id": "efmbajvxtr0pk",
  "issns": [],
  "landing_url": "https://www.biorxiv.org/content/10.64898/2026.10.16.759642v1",
  "language": null,
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "biorxiv",
      "plant biology"
    ],
    [
      "openalex",
      "1312|Photosynthetic Processes and Mechanisms"
    ]
  ],
  "openalex": "W7222983634",
  "pdf_url": "https://www.biorxiv.org/content/10.64898/2026.10.16.759642v1.full.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-16",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/efmbajvxtr0pk.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:28:36Z",
      "native_id": "10.64898/2026.10.16.759642",
      "source": "biorxiv",
      "url": "https://www.biorxiv.org/content/10.64898/2026.10.16.759642v1"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "biorxiv:plant biology",
      "subfield": "plant-biology"
    }
  ],
  "title": "Mass spectrometric identification of a PSI-LHCI-cytochrome b6f complex in both Arabidopsis stn7 and pph1",
  "title_text": "Mass spectrometric identification of a PSI-LHCI-cytochrome b6f complex in both Arabidopsis stn7 and pph1",
  "type": "preprint",
  "updated_at": "2026-10-30T12:28:36Z",
  "url": "https://research.draconitic.com/paper/efmbajvxtr0pk",
  "venue": "bioRxiv",
  "versions": []
}