{
  "abstract": "BackgroundThis study aimed to develop a pretreatment MRI-derived radiogenomic surrogate of a prespecified hypoxia–angiogenesis–epithelial–mesenchymal transition transcriptional state and to evaluate its associations with treatment response and survival in nasopharyngeal carcinoma.MethodsThis multicenter observational study integrated patient-derived public transcriptomic datasets, experimental CNE2/CNE2-IR radioresistance models, a prospective RNA-sequencing cohort of 49 patients with paired contrast-enhanced T1-weighted MRI and RNA data, and two independent clinical cohorts (n = 532 and n = 118). HAE-CAS was the equal-weight mean of three within-cohort standardized module scores. Treatment response was assessed approximately 3 months after chemoradiotherapy according to RECIST version 1.1 using nasopharyngeal MRI, nasopharyngoscopy, and clinical evaluation. Rad-HAE used a five-feature Gaussian ridge model and was evaluated with 20 repetitions of five-fold nested cross-validation; response and survival outcomes were excluded from model development.ResultsAmong 49 response-evaluable RNA-sequenced patients, 17 had complete response (CR) and 32 had partial response (PR); no stable or progressive disease occurred. HAE-CAS was higher in PR than CR (rank-biserial correlation = 0.365, P = 0.035). In GSE102349, continuous HAE-CAS was not significantly associated with progression-free survival (HR per 1-SD increase = 1.20, 95% CI 0.74–1.95; P = 0.465), whereas the median-dichotomized comparison was exploratory (log-rank P = 0.024). Aggregated out-of-fold Rad-HAE correlated with HAE-CAS (Spearman rho = 0.637, P < 0.001) and discriminated PR from CR among 49 paired response-evaluable patients (AUC = 0.846, 95% CI 0.732–0.943). In two independent clinical cohorts, higher Rad-HAE was associated with shorter progression-free and overall survival.ConclusionPretreatment CE-T1WI radiomics was associated with interpatient variation in a prespecified composite HAE transcriptional state. Rad-HAE represents an imaging-based surrogate associated with the composite HAE transcriptional state and does not directly quantify individual biological processes.",
  "abstract_shown": true,
  "affiliations": [],
  "article_type": "Original Research",
  "arxiv": null,
  "author_total": 5,
  "authors": [
    {
      "name": "Zhichao Zuo"
    },
    {
      "name": "Ting Zhu"
    },
    {
      "name": "Yixin Lu"
    },
    {
      "name": "Xiaoyi Zeng"
    },
    {
      "name": "Yang Liu"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/egam0cgpjcqvp.bib",
  "cited_by": 0,
  "doi": "10.3389/fmolb.2026.1968989",
  "fulltext_url": null,
  "id": "egam0cgpjcqvp",
  "issns": [
    "2296-889X"
  ],
  "landing_url": "https://www.frontiersin.org/articles/10.3389/fmolb.2026.1968989",
  "language": null,
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "journal",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "feed-journal",
      "biochemistry/general"
    ]
  ],
  "openalex": null,
  "pdf_url": null,
  "pmcid": null,
  "pmid": null,
  "published": "2026-11-05",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/egam0cgpjcqvp.ris",
  "sources": [
    {
      "first_seen": "2026-11-05T12:20:12Z",
      "native_id": "10.3389/fmolb.2026.1968989",
      "source": "feed-frontiers",
      "url": "https://www.frontiersin.org/articles/10.3389/fmolb.2026.1968989"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "journal-openalex:1312|Protein Structure and Dynamics",
      "subfield": "molecular-biology"
    }
  ],
  "title": "MRI-derived radiogenomic surrogate of a hypoxia–angiogenesis–EMT transcriptional state in nasopharyngeal carcinoma: multicenter external validation",
  "title_text": "MRI-derived radiogenomic surrogate of a hypoxia–angiogenesis–EMT transcriptional state in nasopharyngeal carcinoma: multicenter external validation",
  "type": "peer-reviewed",
  "updated_at": "2026-11-07T00:20:49Z",
  "url": "https://research.draconitic.com/paper/egam0cgpjcqvp",
  "venue": "Frontiers in Molecular Biosciences",
  "versions": []
}