{
  "abstract": "This study capitalizes on computer-assisted semen analysis (CASA) to evaluate a precision nutritional strategy integrating agro-industrial feed upcycling and functional phytogenic supplementation in rams. Black Belly rams (n = 15) sustained on an upcycled basal diet derived from dairy-cow feed rejections were assigned to a non-supplemented control group (CONT) or supplemented with Withania somnifera (WITH) or Lepidium meyenii (LEPI) at 400 mg kg⁻¹ LW d⁻¹. The 64-day trial was conducted from May to July in arid northern Mexico. Sexual drive, seminal quality, and high-resolution sperm kinematics were evaluated using CASA. Body and testicular measurements, ejaculate volume, and most categorical motility traits remained comparable among treatments (p > 0.05). However, phytogenic supplementation enhanced selected seminal and sexual-drive variables (p < 0.05). Crucially, longitudinal treatment × time interactions (p < 0.05) showed that while the CONT group experienced a seasonal decline in local mobility (LOCMOB), it increased from 1.47% to 2.01% in the WITH group, representing a 37% relative increase over time. Moreover, both WITH and LEPI increased LIN and STR by the end of the experimental period (p < 0.05), demonstrating more linear and efficient sperm trajectories. These findings highlight the productive value of upcycled dairy-cow feed rejections and demonstrate that strategic supplementation with W. somnifera or L. meyenii can refine sexual drive, seminal quality, and sperm kinematic efficiency. This integrated bio-circular strategy provides actionable insights for sustainable small-ruminant production under challenging arid summer conditions.",
  "abstract_shown": true,
  "affiliations": [
    "Graduate Program-Natural Resources and Environment in Arid Lands, Regional Universitary Unit on Arid Lands, Chapingo Autonomous University, Bermejillo 35230, Durango, Mexico.",
    "Graduate Program-Agricultural and Livestock Sciences, Laguna Unit, Antonio Narro Agrarian Autonomous University, Torreon 27054, Coahuila, Mexico.",
    "Institute of Agricultural Sciences, Autonomous University of Baja California, Mexicali 21705, Baja California, Mexico.",
    "Division of Health, Biological and Environmental Sciences, Open and Distance University of Mexico, Ciudad de México 03330, Mexico.",
    "Faculty of Chemical Sciences, Juarez University of the State of Durango, Gomez Palacio 35010, Durango, Mexico.",
    "Department of Sustainable and Protected Agriculture, Technological University of Rodeo, Rodeo 35760, Durango, Mexico."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 11,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "César A Meza-Herrera",
      "orcid": "0000-0003-0289-2009"
    },
    {
      "affs": [
        1
      ],
      "name": "Ángeles De Santiago-Miramontes",
      "orcid": "0000-0002-5491-0662"
    },
    {
      "affs": [
        1
      ],
      "name": "Andrés J Rodríguez-Sánchez"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Karla Q Ramírez-Uranga",
      "orcid": "0000-0003-1139-6883"
    },
    {
      "affs": [
        0
      ],
      "name": "Raquel Santos-Silva",
      "orcid": "0009-0001-5874-3881"
    },
    {
      "affs": [
        2
      ],
      "name": "Ulises Macías-Cruz"
    },
    {
      "affs": [
        0
      ],
      "name": "Cayetano Navarrete-Molina",
      "orcid": "0000-0001-7867-2745"
    },
    {
      "affs": [
        1
      ],
      "name": "Denia L Vargas-García"
    },
    {
      "affs": [
        3
      ],
      "name": "Mayela Rodríguez-González"
    },
    {
      "affs": [
        4
      ],
      "name": "Edén A Luna-Zapién"
    },
    {
      "affs": [
        5
      ],
      "name": "María A Sariñana-Navarrete",
      "orcid": "0000-0002-0936-1692"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/ehdsb4y96dxpe.bib",
  "cited_by": 0,
  "doi": "10.3390/vetsci13101009",
  "fulltext_url": "https://europepmc.org/articles/PMC13709658",
  "id": "ehdsb4y96dxpe",
  "issns": [
    "2306-7381"
  ],
  "landing_url": "https://europepmc.org/article/MED/42944105",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "2743|Sperm and Testicular Function"
    ]
  ],
  "openalex": "W7214181373",
  "pdf_url": "https://europepmc.org/articles/PMC13709658?pdf=render",
  "pmcid": "PMC13709658",
  "pmid": "42944105",
  "published": "2026-09-24",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/ehdsb4y96dxpe.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:22:24Z",
      "native_id": "MED:42944105",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42944105"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2743|Sperm and Testicular Function",
      "subfield": "reproductive-child"
    }
  ],
  "title": "Dairy Cattle Feed Upcycling, Adaptogenic Nutraceuticals and Precision Reproduction: Advanced CASA Sperm Kinematic Profiling and Sexual Drive in Rams.",
  "title_text": "Dairy Cattle Feed Upcycling, Adaptogenic Nutraceuticals and Precision Reproduction: Advanced CASA Sperm Kinematic Profiling and Sexual Drive in Rams.",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:22:24Z",
  "url": "https://research.draconitic.com/paper/ehdsb4y96dxpe",
  "venue": "Veterinary sciences",
  "versions": []
}