{
  "abstract": "Polymer nanoparticles (NPs), due to their small size and surface functionalization potential have demonstrated effective drug transport across the blood-brain-barrier (BBB). Currently, the lack of in vitro BBB models that closely recapitulate complex human brain microenvironments contributes to high failure rates of neuropharmaceutical clinical trials. In this work, a previously established microfluidic 3D in vitro human BBB model, formed by the self-assembly of human-induced pluripotent stem cell-derived endothelial cells, primary brain pericytes, and astrocytes in triculture within a 3D fibrin hydrogel is exploited to quantify polymer NP permeability, as a function of size and surface chemistry. Microvasculature are perfused with commercially available 100-400 nm fluorescent polystyrene (PS) NPs, and newly synthesized 100 nm rhodamine-labeled polyurethane (PU) NPs. Confocal images are taken at different timepoints and computationally analyzed to quantify fluorescence intensity inside/outside the microvasculature, to determine NP spatial distribution and permeability in 3D. Results show similar permeability of PS and PU NPs, which increases after surface-functionalization with brain-associated ligand holo-transferrin. Compared to conventional transwell models, the method enables rapid analysis of NP permeability in a physiologically relevant human BBB set-up. Therefore, this work demonstrates a new methodology to preclinically assess NP ability to cross the human BBB.",
  "abstract_shown": true,
  "affiliations": [],
  "article_type": null,
  "arxiv": null,
  "author_total": 8,
  "authors": [
    {
      "name": "Sharon Wei Ling Lee"
    },
    {
      "name": "Marco Campisi"
    },
    {
      "name": "Tatsuya Osaki"
    },
    {
      "name": "Luca Possenti"
    },
    {
      "name": "Clara Mattu"
    },
    {
      "name": "Giulia Adriani"
    },
    {
      "name": "Roger Dale Kamm"
    },
    {
      "name": "Valeria Chiono"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/ehv9bwrr52nz0.bib",
  "cited_by": 104,
  "doi": "10.1002/adhm.201901486",
  "fulltext_url": null,
  "id": "ehv9bwrr52nz0",
  "issns": [
    "2192-2640",
    "2192-2659"
  ],
  "landing_url": "https://hal.science/hal-05776433v1",
  "language": "en",
  "licence": "CC-BY-NC-ND",
  "licence_label": "CC BY-NC-ND",
  "licence_source": "journal",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "hal",
      "0.sdv"
    ],
    [
      "hal",
      "1.sdv.ib"
    ],
    [
      "hal",
      "2.sdv.ib.bio"
    ],
    [
      "openalex",
      "2808|Barrier Structure and Function Studies"
    ]
  ],
  "openalex": "W3009530468",
  "pdf_url": "https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/adhm.201901486",
  "pmcid": null,
  "pmid": "32125776",
  "published": "2026-10-03",
  "publisher": "Wiley",
  "ris": "https://research.draconitic.com/paper/ehv9bwrr52nz0.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:17:46Z",
      "native_id": "hal-05776433",
      "source": "hal",
      "url": "https://hal.science/hal-05776433v1"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "hal:1.sdv.ib",
      "subfield": "systems-synthetic"
    }
  ],
  "title": "Modeling Nanocarrier Transport across a 3D In Vitro Human Blood-Brain–Barrier Microvasculature",
  "title_text": "Modeling Nanocarrier Transport across a 3D In Vitro Human Blood-Brain–Barrier Microvasculature",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:17:46Z",
  "url": "https://research.draconitic.com/paper/ehv9bwrr52nz0",
  "venue": "Advanced Healthcare Materials",
  "versions": []
}