{
  "abstract": "Age-related degeneration remains a premier biomedical challenge, frequently underpinned by cellular senescence and stem cell dysfunction. Accumulating evidence suggests that umbilical cord mesenchymal stromal cell (UC-MSC)-derived extracellular vesicles (UC-EVs) and the longevity protein Klotho hold great promise as anti-aging biotherapeutics. Here, we strategically engineered UC-MSCs to overexpress Klotho and generated functional Klotho-enriched UC-EVs (KL-EVs). Our data demonstrated that genetic modification endowed UC-MSCs with prominent osteogenic differentiation potency and angiogenesis efficacy. More importantly, KL-EVs profoundly rescued multifaceted senescent phenotypes in both adult bone marrow mesenchymal stem cells (BMSCs) and human renal tubular epithelial cells (HK-2) in vitro. In particular, KL-EVs rescued the age-related differentiation bias of adult BMSCs by robustly prompting their osteogenic differentiation capacity. Taken together, we developed a novel type of UC-MSCs and isolated their EVs enriched with Klotho protein. This strategy integrated and enhanced the anti-aging effects of both entities in vitro, offering a new perspective for counteracting cellular senescence and alleviating age-associated degenerative alterations.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Rheumatology and Immunology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China",
    "Department of Hematology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China",
    "Department of Hematology, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430014, China"
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 9,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Anyuan Zhang"
    },
    {
      "affs": [
        0
      ],
      "name": "Shan Wang"
    },
    {
      "affs": [
        0
      ],
      "name": "Jing Zhang"
    },
    {
      "affs": [
        0
      ],
      "name": "Bianlei Yang"
    },
    {
      "affs": [
        1
      ],
      "name": "Xiaorong Su"
    },
    {
      "affs": [
        0
      ],
      "name": "Wenying Deng"
    },
    {
      "affs": [
        2
      ],
      "name": "Hongxiang Wang",
      "orcid": "0000-0002-2499-3604"
    },
    {
      "affs": [
        1
      ],
      "name": "Zhichao Chen",
      "orcid": "0000-0002-4900-7057"
    },
    {
      "affs": [
        0
      ],
      "name": "Qiubai Li",
      "orcid": "0000-0001-7884-0745"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/ehyaeqrkzyxxz.bib",
  "cited_by": 0,
  "doi": "10.3390/ijms27188326",
  "fulltext_url": null,
  "id": "ehyaeqrkzyxxz",
  "issns": [
    "1422-0067"
  ],
  "landing_url": "https://www.mdpi.com/1422-0067/27/18/8326",
  "language": null,
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "2716|Mesenchymal stem cell research"
    ]
  ],
  "openalex": "W7214475184",
  "pdf_url": null,
  "pmcid": null,
  "pmid": null,
  "published": "2026-09-19",
  "publisher": "MDPI AG",
  "ris": "https://research.draconitic.com/paper/ehyaeqrkzyxxz.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:18:55Z",
      "native_id": "10.3390/ijms27188326",
      "source": "crossref",
      "url": "https://www.mdpi.com/1422-0067/27/18/8326"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2716|Mesenchymal stem cell research",
      "subfield": "human-genetics"
    }
  ],
  "title": "Characterization of Umbilical Cord Mesenchymal Stromal Cells Overexpressing Klotho and In Vitro Anti-Aging Efficacy of Their Derived Extracellular Vesicles",
  "title_text": "Characterization of Umbilical Cord Mesenchymal Stromal Cells Overexpressing Klotho and In Vitro Anti-Aging Efficacy of Their Derived Extracellular Vesicles",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:18:55Z",
  "url": "https://research.draconitic.com/paper/ehyaeqrkzyxxz",
  "venue": "International Journal of Molecular Sciences",
  "versions": []
}