{
  "abstract": "Imported falciparum malaria remains an important challenge in China, particularly with the emergence of antimalarial resistance in Africa. This study characterized resistance-associated mutations in pfk13, pfcrt and pfmdr1 by nested PCR and Sanger sequencing of 291 Plasmodium falciparum (P. falciparum) isolates imported from 29 African countries into Guangdong Province during 2023-2025. Sequences were obtained for pfk13 (n = 279), pfcrt (n = 225) and pfmdr1 (n = 235). Four pfk13 variants-C469Y, Y558C, R561H and A675V-were each detected once (0.36%). These isolates originated from East and Central Africa. The wild-type pfk13 haplotype accounted for 98.57% of isolates. For pfcrt, the wild-type MNK haplotype predominated (85.33%), whereas the IET triple-mutant haplotype accounted for 14.22% and was mainly detected in isolates from West and Central Africa. Y184F was the predominant pfmdr1 variant (55.32%), with an annual prevalence of 52.59-60.78%. Other variants included N86Y (3.40%), D1246Y (1.28%), and S163N, T199S and S1217F (0.43% each). The Y184F single-mutant haplotype was most common (51.91%). The imported isolates showed a low prevalence of pfk13 variants, predominance of wild-type pfcrt and frequent pfmdr1 Y184F variants. The detection of several pfk13 variants highlights the need for continued molecular surveillance of antimalarial resistance.",
  "abstract_shown": true,
  "affiliations": [
    "Parasitic Disease Prevention and Control Institute, Guangdong Center for Disease Control and Prevention, Guangzhou 511430, China.",
    "State Key Laboratory for Biocontrol, School of Medicine, Shenzhen Campus, Sun Yat-sen University, Shenzhen 518107, China.",
    "Shenzhen Key Laboratory for Systems Medicine in Inflammatory Diseases, Shenzhen Campus, Sun Yat-sen University, Shenzhen 518107, China.",
    "National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, School of Medicine, Shenzhen Campus, Sun Yat-sen University, Shenzhen 518107, China."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 11,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Lijun Chen"
    },
    {
      "affs": [
        0
      ],
      "name": "Qiang Mao"
    },
    {
      "affs": [
        0
      ],
      "name": "De Wu"
    },
    {
      "affs": [
        0
      ],
      "name": "Jiahui Liu"
    },
    {
      "affs": [
        0
      ],
      "name": "Jiayi Zhang"
    },
    {
      "affs": [
        0
      ],
      "name": "Wencheng Lu"
    },
    {
      "affs": [
        0
      ],
      "name": "Minwu Peng"
    },
    {
      "affs": [
        0
      ],
      "name": "Yating Chen"
    },
    {
      "affs": [
        0
      ],
      "name": "Jieling Wang"
    },
    {
      "affs": [
        0
      ],
      "name": "Jingdiao Chen"
    },
    {
      "affs": [
        0,
        1,
        2,
        3
      ],
      "name": "Mang Shi",
      "orcid": "0000-0002-6154-4437"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/ej5d9h28h75wn.bib",
  "cited_by": 0,
  "doi": "10.3390/pathogens15101080",
  "fulltext_url": "https://europepmc.org/articles/PMC13708556",
  "id": "ej5d9h28h75wn",
  "issns": [
    "2076-0817"
  ],
  "landing_url": "https://europepmc.org/article/MED/42931231",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "2739|Malaria Research and Control"
    ]
  ],
  "openalex": "W7221775922",
  "pdf_url": "https://europepmc.org/articles/PMC13708556?pdf=render",
  "pmcid": "PMC13708556",
  "pmid": "42931231",
  "published": "2026-10-14",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/ej5d9h28h75wn.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:22:01Z",
      "native_id": "MED:42931231",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42931231"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2739|Malaria Research and Control",
      "subfield": "epidemiology-public-health"
    }
  ],
  "title": "Molecular Surveillance of Antimalarial Drug Resistance-Associated Mutations in P. falciparum Imported from Africa into Guangdong Province, China, 2023-2025.",
  "title_text": "Molecular Surveillance of Antimalarial Drug Resistance-Associated Mutations in P. falciparum Imported from Africa into Guangdong Province, China, 2023-2025.",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:22:01Z",
  "url": "https://research.draconitic.com/paper/ej5d9h28h75wn",
  "venue": "Pathogens (Basel, Switzerland)",
  "versions": []
}