{
  "abstract": "Multiple myeloma (MM) depends on a permissive bone marrow microenvironment that supports malignant plasma cell survival and disease progression. Bone marrow mesenchymal stromal cells (BM-MSCs) are key components of this niche, but the mechanisms by which they are recruited by MM cells remain unclear. Here, we show that the MM-derived secretome (MM-Sec) promotes BM-MSC wound closure and transwell migration-associated responses in vitro. A small extracellular vesicle (sEV)-enriched pellet was isolated from MM.1S-Sec by differential ultracentrifugation and characterized, and the relative contributions of the sEV-enriched fraction and sEV-depleted secretome (post-100,000× g) to BM-MSC migration were assessed. The results show that the sEV-depleted secretome is more effective than the sEV-enriched fraction in promoting BM-MSC recruitment. Chemokine profiling of MM.1S-Sec identified CCL3 (MIP-1α) and CCL5 (RANTES) as abundant secreted factors and exploratory candidate contributors. Recombinant CCL3 and CCL5 increased the BM-MSC transwell migration response at high concentrations. Priming with MM.1S-Sec was associated with increased transwell migration-associated responses in the presence of CCL3 and CCL5. Together, these exploratory findings suggest that both vesicle-associated and soluble non-vesicular components may contribute to BM-MSC migration, while CCL3 and CCL5 may participate as candidate contributors within a broader network of soluble mediators. These findings identify secretome-mediated modulation of stromal migration as a relevant component of MM–microenvironment crosstalk that warrants further mechanistic investigation.",
  "abstract_shown": true,
  "affiliations": [
    "i3S, Instituto de Investigação e Inovação em Saúde, , Rua Alfredo Allen, 208, 4200-135 Porto, Portugal"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 7,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Maria João Fernandes"
    },
    {
      "affs": [
        0
      ],
      "name": "Andrea Giestinhas"
    },
    {
      "affs": [
        0
      ],
      "name": "Sara Reis Moura"
    },
    {
      "affs": [
        0
      ],
      "name": "Maria Helena Vasconcelos"
    },
    {
      "affs": [
        0
      ],
      "name": "Susana Gomes Santos"
    },
    {
      "affs": [
        0
      ],
      "name": "Maria José Oliveira"
    },
    {
      "affs": [
        0
      ],
      "name": "Maria Inês Almeida"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/ejeb9e4937pj4.bib",
  "cited_by": 0,
  "doi": "10.3390/ijms27198763",
  "fulltext_url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13665306/?tool=EBI",
  "id": "ejeb9e4937pj4",
  "issns": [
    "1422-0067"
  ],
  "landing_url": "https://europepmc.org/article/PMC/PMC13665306",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "2720|Multiple Myeloma Research and Treatments"
    ]
  ],
  "openalex": "W7214960597",
  "pdf_url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13665306/pdf/?tool=EBI",
  "pmcid": "PMC13665306",
  "pmid": null,
  "published": "2026-09-30",
  "publisher": "MDPI AG",
  "ris": "https://research.draconitic.com/paper/ejeb9e4937pj4.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:17:35Z",
      "native_id": "PMC:PMC13665306",
      "source": "europepmc",
      "url": "https://europepmc.org/article/PMC/PMC13665306"
    },
    {
      "first_seen": "2026-10-30T12:18:55Z",
      "native_id": "10.3390/ijms27198763",
      "source": "crossref",
      "url": "https://www.mdpi.com/1422-0067/27/19/8763"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2720|Multiple Myeloma Research and Treatments",
      "subfield": "general"
    }
  ],
  "title": "Multiple Myeloma Cell-Derived Secretome Modulates Bone Marrow Mesenchymal Stromal Cell Migration In Vitro",
  "title_text": "Multiple Myeloma Cell-Derived Secretome Modulates Bone Marrow Mesenchymal Stromal Cell Migration In Vitro",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:18:55Z",
  "url": "https://research.draconitic.com/paper/ejeb9e4937pj4",
  "venue": "International journal of molecular sciences",
  "versions": []
}