{
  "abstract": "Background: The bacterial genus Streptomyces comprises well-known producers of bioactive compounds, mainly antibiotics. Specifically, the strain Streptomyces sp. VITGV100 shows promise as a potent antimicrobial source. This study investigates the activation of cryptic biosynthetic genes within this strain through chemical elicitation to amplify the production of secondary metabolites.\n\nMaterials and methods: To achieve this, Streptomyces sp. VITGV100 was cultured in nutrient broth supplemented with 0.5% dimethyl sulfoxide (DMSO) to elicit metabolic changes over 7, 14, and 21 days. The resulting crude extracts were screened for antimicrobial activity against four human pathogens: Escherichia coli (Microbial Type Culture Collection and Gene Bank [MTCC] 1687), Pseudomonas aeruginosa (MTCC 3541), Bacillus subtilis (MTCC 2756), and Staphylococcus aureus (MTCC 737). Bioactive fractions were characterized by gas chromatography-mass spectrometry (GC-MS). Finally, identified compounds, namely lacthydrazide, were docked against target proteins: E. coli Z-ring-associated protein (5IMJ), P. aeruginosa OXA10 (4WZ5), S. aureus TyrRS (1JIJ), and B. subtilis DLTA (3E7X).\n\nResults: The 7-day crude extract exhibited the strongest antimicrobial activity, yielding a maximum inhibition zone of 31 mm against E. coli at 100 μg/ml and a minimum zone of 9 mm against B. subtilis at 25 μg/ml. GC-MS analysis revealed 45 distinct peaks in the included extracts versus 30 in controls, identifying lacthydrazide as a primary compound. Molecular docking confirmed the strong binding affinity of lacthydrazide to the target proteins, supporting its antimicrobial potential.\n\nConclusions: Streptomyces sp. VITGV100, elicited with 0.5% DMSO, effectively activated cryptic genes to synthesize potent antibacterial compounds. These findings position this strain as a valuable resource for discovering novel antimicrobials.",
  "abstract_shown": true,
  "affiliations": [
    "School of BioSciences and Technology, Vellore Institute of Technology, VIT University, Vellore, Tamil Nadu, India"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 2,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Madhuri Mukindrao Moon"
    },
    {
      "affs": [
        0
      ],
      "name": "John Godwin Christopher"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/ekp0gwazsrfqz.bib",
  "cited_by": 0,
  "doi": "10.5114/bta/224355",
  "fulltext_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13725489/",
  "id": "ekp0gwazsrfqz",
  "issns": [
    "0860-7796",
    "2353-9461"
  ],
  "landing_url": "https://doi.org/10.5114/bta/224355",
  "language": "en",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Research Paper"
    ],
    [
      "openalex",
      "2736|Microbial Natural Products and Biosynthesis"
    ]
  ],
  "openalex": "W7213932179",
  "pdf_url": "https://www.biotechnologia-journal.org/pdf-224355-145394?filename=GC-MS-identification-of-a.pdf",
  "pmcid": "PMC13725489",
  "pmid": "42973894",
  "published": "2026-09-21",
  "publisher": "Termedia Publishing",
  "ris": "https://research.draconitic.com/paper/ekp0gwazsrfqz.ris",
  "sources": [
    {
      "first_seen": "2026-11-04T12:23:52Z",
      "native_id": "PMC13725489",
      "source": "pmc",
      "url": "https://doi.org/10.5114/bta/224355"
    },
    {
      "first_seen": "2026-11-04T15:20:49Z",
      "native_id": "MED:42973894",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42973894"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2736|Microbial Natural Products and Biosynthesis",
      "subfield": "pharmacology-toxicology"
    }
  ],
  "title": "GC-MS identification of a lacthydrazide-class siderophore produced by Streptomyces sp. VITGV100: its antimicrobial activity and molecular docking",
  "title_text": "GC-MS identification of a lacthydrazide-class siderophore produced by Streptomyces sp. VITGV100: its antimicrobial activity and molecular docking",
  "type": "peer-reviewed",
  "updated_at": "2026-11-06T12:21:44Z",
  "url": "https://research.draconitic.com/paper/ekp0gwazsrfqz",
  "venue": "BioTechnologia",
  "versions": []
}