{
  "abstract": "Antiviral monoclonal antibodies (mAbs) rely on Fc-mediated effector functions, which depend on interactions with Fc receptors (FcRs) expressed on immune cells such as monocytes and dendritic cells (DCs). While combinations of antiviral mAbs with type I interferons (IFN-I) or IFN inducers are being explored to enhance therapeutic efficacy, the impact of these immunomodulators on FcR expression remains poorly understood. In this study, we investigated the effects of IFN-α2, IFN-β, and the IFN-inducer TAK-981 (a SUMOylation inhibitor) on FcR modulation and functional activation in human monocyte subsets and monocyte-derived DCs (moDCs). Using flow cytometry and imaging techniques, we mapped the expression of activating FcγRs (CD64, CD32A, CD16A) and neonatal FcR (FcRn) under steady-state and stimulated conditions. We found that IFN-I selectively downregulates CD16A expression in classical monocytes while preserving other FcRs, whereas TAK-981 reduces FcRn expression in classical monocytes without affecting CD16A. Additionally, IFN-β and TAK-981 but not IFN-α2, selectively enhanced FcγRI expression in moDC. IFN-β also induced a stronger maturation and functional activation program in monocytes and moDCs compared with IFN-α2, whereas TAK-981 largely reproduced the IFN-β-driven phenotype and secretory profile. Furthermore, we demonstrated that IFN-I signaling alters the spatial distribution of FcRs, with IFN-β and TAK-981 promoting distinct redistribution patterns of FcγRs in monocytes and moDCs. These findings reveal a novel layer of FcR regulation, highlighting that IFN-I and IFN inducers differentially modulate FcR expression, localization, and functional activation in a cell-type- and stimulus-specific manner. Overall, this work provides a framework for understanding how IFN-I-based immunotherapies may influence Fc-mediated immune functions while preserving FcRn-dependent antibody persistence.",
  "abstract_shown": true,
  "affiliations": [],
  "article_type": "New results",
  "arxiv": null,
  "author_total": 6,
  "authors": [
    {
      "name": "Rémi Bru"
    },
    {
      "name": "Océane Paris"
    },
    {
      "name": "Guillaume Bossis"
    },
    {
      "name": "Stephanie Boireau"
    },
    {
      "name": "Mar Naranjo-Gomez"
    },
    {
      "name": "Mireia Pelegrin"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/eme5gwxx627xf.bib",
  "cited_by": 0,
  "doi": "10.64898/2026.10.01.756027",
  "fulltext_url": "https://www.biorxiv.org/content/10.64898/2026.10.01.756027v1.full",
  "id": "eme5gwxx627xf",
  "issns": [],
  "landing_url": "https://hal.science/hal-05841901v1",
  "language": "en",
  "licence": "CC-BY-NC-ND-4.0",
  "licence_label": "CC BY-NC-ND 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "biorxiv",
      "immunology"
    ],
    [
      "hal",
      "0.sdv"
    ],
    [
      "hal",
      "1.sdv.bbm"
    ],
    [
      "hal",
      "1.sdv.imm"
    ],
    [
      "hal",
      "1.sdv.mhep"
    ],
    [
      "hal",
      "2.sdv.bbm.gtp"
    ],
    [
      "hal",
      "2.sdv.imm.imm"
    ],
    [
      "hal",
      "2.sdv.mhep.mi"
    ],
    [
      "openalex",
      "2741|Monoclonal and Polyclonal Antibodies Research"
    ]
  ],
  "openalex": "W7220853842",
  "pdf_url": "https://hal.science/hal-05841901/document",
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-01",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/eme5gwxx627xf.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:18:02Z",
      "native_id": "hal-05841901",
      "source": "hal",
      "url": "https://hal.science/hal-05841901v1"
    },
    {
      "first_seen": "2026-10-30T12:26:28Z",
      "native_id": "10.64898/2026.10.01.756027",
      "source": "biorxiv",
      "url": "https://www.biorxiv.org/content/10.64898/2026.10.01.756027v1"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "hal:2.sdv.bbm.gtp",
      "subfield": "proteomics-metabolomics"
    },
    {
      "field": "biology",
      "origin": "biorxiv:immunology",
      "subfield": "immunology"
    },
    {
      "field": "medicine",
      "origin": "hal:2.sdv.mhep.mi",
      "subfield": "infectious-disease"
    }
  ],
  "title": "Differential Effects of Distinct Type I Interferons and IFN Inducers on Fc Receptor landscape and myeloid cell function",
  "title_text": "Differential Effects of Distinct Type I Interferons and IFN Inducers on Fc Receptor landscape and myeloid cell function",
  "type": "preprint",
  "updated_at": "2026-10-30T12:26:28Z",
  "url": "https://research.draconitic.com/paper/eme5gwxx627xf",
  "venue": "HAL",
  "versions": []
}