{
  "abstract": "Osteoporosis is a process of bone tissue destruction jointly caused by chronic inflammation and oxidative stress. Indole-3-propionic acid (IPA), as a metabolic molecule of the gut microbiota, exerts anti-inflammatory and antioxidant properties. However, the role of IPA in ovariectomy-induced osteoporosis in rats remains unclear. We utilized ELISA and qRT-PCR to measure cytokine levels in serum and the mRNA expression of bone metabolism-related genes, respectively. Meanwhile, bone microstructure was analyzed using CT scanning technology. Additionally, we conducted non-targeted metabolomics and 16 S rDNA gene sequencing studies on mouse fecal samples, employing multivariate statistical analysis for data visualization. IPA administration significantly attenuated the elevation of inflammatory mediators (TNF-α, IL-1β, IL-6) in OVX rats, while enhancing anti-inflammatory IL-10 levels. It could alter biochemical markers (Ca, P, ALP, CTX-1 and PINP) and suppress the expression of critical osteoclast differentiation genes (NFATc1, CTSK, ACP5, OSCAR), preventing OVX-induced bone loss. Additionally, the bone microstructure improved. Metabolomic profiling revealed IPA’s regulatory role in fatty acid and amino acid metabolic pathways. Phloretin may serve as biomarker for the bone-protective effects of IPA. Additionally, IPA restored the balance of the Bacteroidota/Firmicutes gut microbiota. These findings underscore IPA’s therapeutic potential, providing a scientific rationale for developing IPA-based interventions for osteoporosis management.",
  "abstract_shown": true,
  "affiliations": [
    "Central Hospital Affiliated to Shenyang Medical College",
    "General Hospital of Northern Theater Command"
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 5,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Liang A."
    },
    {
      "affs": [
        1
      ],
      "name": "Na Gong"
    },
    {
      "affs": [
        0
      ],
      "name": "Sheng Li"
    },
    {
      "affs": [
        0
      ],
      "name": "Wenzheng Lv"
    },
    {
      "affs": [
        0
      ],
      "name": "Yingnan Ji"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/eppftw57hp18c.bib",
  "cited_by": 0,
  "doi": "10.1038/s41598-026-61654-9",
  "fulltext_url": "https://doi.org/10.1038/s41598-026-61654-9",
  "id": "eppftw57hp18c",
  "issns": [
    "2045-2322"
  ],
  "landing_url": "https://doi.org/10.1038/s41598-026-61654-9",
  "language": "en",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "journal",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "lcc",
      "Q"
    ],
    [
      "lcc",
      "R"
    ],
    [
      "openalex",
      "1312|Bone Metabolism and Diseases"
    ]
  ],
  "openalex": "W7131095916",
  "pdf_url": null,
  "pmcid": null,
  "pmid": null,
  "published": "2026-08-01",
  "publisher": "Nature Portfolio",
  "ris": "https://research.draconitic.com/paper/eppftw57hp18c.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T13:45:42Z",
      "native_id": "2fdb7367020940d2ac75007a4807d15e",
      "source": "doaj",
      "url": "https://doi.org/10.1038/s41598-026-61654-9"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|Bone Metabolism and Diseases",
      "subfield": "molecular-biology"
    }
  ],
  "title": "Combined metabolomics and microbiota analysis reveals the therapeutic effect of indole-3-propionic acid on ovariectomy-induced osteoporosis",
  "title_text": "Combined metabolomics and microbiota analysis reveals the therapeutic effect of indole-3-propionic acid on ovariectomy-induced osteoporosis",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T13:45:42Z",
  "url": "https://research.draconitic.com/paper/eppftw57hp18c",
  "venue": "Scientific Reports",
  "versions": []
}