{
  "abstract": "This study investigated the effects of dietary N-carbamylglutamate (NCG) supplementation on laying performance, egg quality, lipid metabolism, and antioxidant capacity in Huoyan geese, and explored the underlying molecular mechanisms through liver transcriptomics. 240 female Huoyan geese of similar body weight and age were randomly assigned to four groups: a control group fed a basal diet and three groups fed the basal diet supplemented with 0.06%, 0.08%, or 0.12% NCG. Each group comprised six replicates of 10 geese. The trial lasted 127 d (7-d adaptation). At the end, laying performance and egg quality were assessed, and serum and liver samples were collected for lipid metabolism and antioxidant biomarker analyses. Liver transcriptomes of the control and N1 groups were sequenced. The 0.06% NCG (N1) group exhibited significantly higher laying rate, average egg weight, eggshell strength, eggshell thickness, yolk weight, and serum high-density lipoprotein cholesterol (HDL-C) levels than the control (P < 0.05). Serum total cholesterol (T-CHO), triglyceride (TG), and liver T-CHO, TG, and low-density lipoprotein cholesterol (LDL-C) were significantly decreased in the N1 group (P < 0.05). Superoxide dismutase (SOD) activity was significantly enhanced in serum and liver (P < 0.05). The response was not monotonic: 0.06% NCG produced the greatest improvements, whereas 0.12% did not differ significantly from the control for most variables. Transcriptomic analysis identified 1,041 differentially expressed genes (DEGs) between groups: 137 upregulated and 904 downregulated in N1. After correction for multiple testing, fructose and mannose metabolism was the only significantly enriched KEGG pathway, and enriched Gene Ontology terms were dominated by ribosome- and mitochondrial respiratory chain-related functions. Gene set enrichment analysis (GSEA) further showed that the ribosome gene set was significantly suppressed in the NCG group, whereas no gene set was significantly upregulated. In conclusion, dietary supplementation with 0.06% NCG improved laying performance, egg quality, lipid metabolism, and antioxidant capacity in Huoyan geese, accompanied by hepatic transcriptomic changes associated with lipid and energy metabolism, antioxidant defense, and mitochondrial function.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Animal Science, College of Agriculture, Jinzhou Medical University, Jinzhou City, Liaoning Province, 121000, China",
    "Liaoning Provincial Key Laboratory of Animal Product Quality and Safety, Jinzhou Medical University, Jinzhou City, Liaoning Province, 121000, China",
    "Liaoning Provincial Rural and Agricultural Development Service Center, Shenyang City, Liaoning Province, 110000, China",
    "Liaoning Provincial Modern Agricultural Production Base Construction Engineering Center, Liaoyang City, Liaoning Province, 111000, China"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 10,
  "authors": [
    {
      "affs": [
        0,
        1
      ],
      "name": "Xiaobing Peng"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Xuan Zhao"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Yizeng Lin"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Meng Sun"
    },
    {
      "affs": [
        2
      ],
      "name": "Di Han"
    },
    {
      "affs": [
        3
      ],
      "name": "Changjiang Li"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Ying Wang"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Chunchi Yan"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Wei Ma"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Chunqiang Wang"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/et5f5ms7476nx.bib",
  "cited_by": 0,
  "doi": "10.1016/j.psj.2026.107981",
  "fulltext_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13721930/",
  "id": "et5f5ms7476nx",
  "issns": [
    "0032-5791",
    "1525-3171"
  ],
  "landing_url": "https://doi.org/10.1016/j.psj.2026.107981",
  "language": "en",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Full-Length Article"
    ],
    [
      "openalex",
      "1103|Animal Nutrition and Physiology"
    ]
  ],
  "openalex": "W7222789375",
  "pdf_url": null,
  "pmcid": "PMC13721930",
  "pmid": "42925644",
  "published": "2026-10-17",
  "publisher": "Elsevier",
  "ris": "https://research.draconitic.com/paper/et5f5ms7476nx.ris",
  "sources": [
    {
      "first_seen": "2026-11-03T12:22:32Z",
      "native_id": "PMC13721930",
      "source": "pmc",
      "url": "https://doi.org/10.1016/j.psj.2026.107981"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "openalex:1103|Animal Nutrition and Physiology",
      "subfield": "zoology-behaviour"
    }
  ],
  "title": "Dietary N-carbamylglutamate supplementation improves laying performance, lipid metabolism, and antioxidant capacity in Huoyan geese: insights from liver transcriptomics",
  "title_text": "Dietary N-carbamylglutamate supplementation improves laying performance, lipid metabolism, and antioxidant capacity in Huoyan geese: insights from liver transcriptomics",
  "type": "peer-reviewed",
  "updated_at": "2026-11-03T12:22:32Z",
  "url": "https://research.draconitic.com/paper/et5f5ms7476nx",
  "venue": "Poultry Science",
  "versions": []
}