{
  "abstract": "Fluorescence-guided surgery improves intraoperative brain tumor visualization, but currently available agents remain unreliable for several common entities, particularly lower-grade gliomas and lesions without visible 5-aminolevulinic acid (5-ALA)-induced fluorescence. We prospectively analyzed 69 surgically obtained tumor specimens from 61 patients with WHO grade 4 gliomas, WHO grade 2/3 gliomas, meningiomas, and brain metastases before and after ex vivo incubation in 4 µM water-soluble high-load hypericin-polyvinylpyrrolidone complex (HHL-PVP). Fluorescence lifetime and intensity were quantified using a dual-tap CMOS camera system with a hypericin-specific 575–615 nm bandpass filter. HHL-PVP incubation significantly increased both fluorescence intensity and lifetime in all tumor entities, including specimens without visible 5-ALA fluorescence. An independently derived combined lifetime/intensity regression model discriminated pre- from post-incubation measurements with an area under the receiver operating characteristic curve of 0.975, sensitivity of 98.6%, and specificity of 82.6%; entity-specific areas under the curve ranged from 0.933 to 1.000. These findings support robust hypericin-associated signal detection across major brain tumor entities after ex vivo incubation and provide a rationale for future in vivo evaluation of HHL-PVP in fluorescence-guided neuro-oncological surgery.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Neurosurgery, Medical University Vienna",
    "Department of Medical Physics, Medical University of Vienna",
    "Hypericum LifeScience GmbH",
    "Diagnostic and Research Institute of Pathology, Medical University of Graz"
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 16,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Mario Mischkulnig"
    },
    {
      "affs": [
        1
      ],
      "name": "David Reichert"
    },
    {
      "affs": [
        2
      ],
      "name": "Lionel Wightman"
    },
    {
      "affs": [
        0
      ],
      "name": "Vanessa Roth"
    },
    {
      "affs": [
        0
      ],
      "name": "Marijke Hoelz"
    },
    {
      "affs": [
        0
      ],
      "name": "Alexandra Klang"
    },
    {
      "affs": [
        0
      ],
      "name": "Lisa I. Körner"
    },
    {
      "affs": [
        0
      ],
      "name": "Barbara Kiesel"
    },
    {
      "affs": [
        3
      ],
      "name": "Djenana Vejzovic"
    },
    {
      "affs": [
        1
      ],
      "name": "Mikael T. Erkkilae"
    },
    {
      "affs": [
        1
      ],
      "name": "Angelika Unterhuber"
    },
    {
      "affs": [
        1
      ],
      "name": "Rainer Leitgeb"
    },
    {
      "affs": [
        3
      ],
      "name": "Beate Rinner"
    },
    {
      "affs": [
        2
      ],
      "name": "Andreas Kubin"
    },
    {
      "affs": [
        1
      ],
      "name": "Marco Andreana"
    },
    {
      "affs": [
        0
      ],
      "name": "Georg Widhalm"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/evpsm6kqpzykk.bib",
  "cited_by": 0,
  "doi": "10.1038/s41598-026-62969-3",
  "fulltext_url": "https://doi.org/10.1038/s41598-026-62969-3",
  "id": "evpsm6kqpzykk",
  "issns": [
    "2045-2322"
  ],
  "landing_url": "https://doi.org/10.1038/s41598-026-62969-3",
  "language": "en",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "journal",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "lcc",
      "Q"
    ],
    [
      "lcc",
      "R"
    ],
    [
      "openalex",
      "2740|Photodynamic Therapy Research Studies"
    ]
  ],
  "openalex": "W7169793500",
  "pdf_url": null,
  "pmcid": null,
  "pmid": null,
  "published": "2026-07-01",
  "publisher": "Nature Portfolio",
  "ris": "https://research.draconitic.com/paper/evpsm6kqpzykk.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T13:45:52Z",
      "native_id": "0e1d46ad37c7447fa207d66b46062249",
      "source": "doaj",
      "url": "https://doi.org/10.1038/s41598-026-62969-3"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2740|Photodynamic Therapy Research Studies",
      "subfield": "general"
    }
  ],
  "title": "Fluorescence lifetime and intensity detection of water-soluble hypericin accumulation in ex-vivo glioma, meningioma, and brain metastasis tissue",
  "title_text": "Fluorescence lifetime and intensity detection of water-soluble hypericin accumulation in ex-vivo glioma, meningioma, and brain metastasis tissue",
  "type": "peer-reviewed",
  "updated_at": "2026-10-31T14:20:43Z",
  "url": "https://research.draconitic.com/paper/evpsm6kqpzykk",
  "venue": "Scientific Reports",
  "versions": []
}