{
  "abstract": "Cryptosporidium spp. and Giardia duodenalis are important parasitic protozoa infecting humans and a wide range of animal species. Some species and assemblages have zoonotic potential and pose health risks to both humans and animals. This study aimed to molecularly detect and characterize Cryptosporidium spp. and G. duodenalis and assess their zoonotic potential in pigs, cattle, cats and wildlife. Nested PCR and sequence analyses of the small subunit ribosomal RNA (ssu rRNA) gene for Cryptosporidium spp. and ssu rRNA, β-giardin (bg), glutamate dehydrogenase (gdh) and triosephosphate isomerase (tpi) genes for G. duodenalis were used for molecular detection and characterization. The PCR positivity rate for Cryptosporidium was 17.9% (12/67) in pigs, whereas the corresponding rates for G. duodenalis were 6.0% (4/67) in pigs and 2.5% (2/80) in cats. Neither parasite was detected in cattle (0/61) or wildlife (0/144). Cryptosporidium scrofarum, a potentially zoonotic species, was the only Cryptosporidium species identified. Among the four Giardia-positive pig samples, assemblages B and E were each identified in two samples. Co-infection with C. scrofarum and assemblage E was detected in one pig. Feline-associated assemblage F was identified in two cat samples. Potentially zoonotic C. scrofarum and zoonotic G. duodenalis assemblage B were detected in pigs, together with livestock-associated assemblage E. Feline-associated assemblage F was identified in cats. These findings provide historical, sample-specific molecular data on the animals examined.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Protozoology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.",
    "National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Japan.",
    "Department of General Medicine, Faculty of Medicine, Juntendo University, Tokyo, Japan.",
    "Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.",
    "Department of Parasitology, Faculty of Medicine, Kasetsart University, Bangkok, Thailand.",
    "Department of Pre-Clinic and Applied Animal Science, Faculty of Veterinary Science, Mahidol University, Nakhon Pathom, Thailand.",
    "Laboratory of Parasitology, Department of Bacteria, Parasites and Fungi, Statens Serum Institut, Copenhagen S, Denmark."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 10,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Ruenruetai Udonsom",
      "orcid": "0000-0002-0785-5242"
    },
    {
      "affs": [
        1
      ],
      "name": "Yoshifumi Nishikawa"
    },
    {
      "affs": [
        0
      ],
      "name": "Aongart Mahittikorn",
      "orcid": "0000-0001-6328-4213"
    },
    {
      "affs": [
        0,
        2
      ],
      "name": "Repeepun Prasertbun",
      "orcid": "0009-0003-0860-3068"
    },
    {
      "affs": [
        0,
        2
      ],
      "name": "Hirotake Mori"
    },
    {
      "affs": [
        3
      ],
      "name": "Nathamon Ngaosuwankul",
      "orcid": "0000-0001-7465-0696"
    },
    {
      "affs": [
        4
      ],
      "name": "Ai-Rada Pintong",
      "orcid": "0009-0008-2342-4515"
    },
    {
      "affs": [
        5
      ],
      "name": "Charoonluk Jirapattharasate"
    },
    {
      "affs": [
        6
      ],
      "name": "Christen Rune Stensvold",
      "orcid": "0000-0002-1417-7048"
    },
    {
      "affs": [
        0
      ],
      "name": "Supaluk Popruk",
      "orcid": "0000-0003-4218-9633"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/ewm2djhenvm6p.bib",
  "cited_by": 0,
  "doi": "10.1002/vms3.71327",
  "fulltext_url": "https://europepmc.org/articles/PMC13744390",
  "id": "ewm2djhenvm6p",
  "issns": [
    "2053-1095"
  ],
  "landing_url": "https://europepmc.org/article/MED/42998259",
  "language": "eng",
  "licence": "CC-BY-NC-ND",
  "licence_label": "CC BY-NC-ND",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "2405|Parasitic Infections and Diagnostics"
    ]
  ],
  "openalex": "W7225459207",
  "pdf_url": "https://europepmc.org/articles/PMC13744390?pdf=render",
  "pmcid": "PMC13744390",
  "pmid": "42998259",
  "published": "2026-11-01",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/ewm2djhenvm6p.ris",
  "sources": [
    {
      "first_seen": "2026-11-09T15:20:34Z",
      "native_id": "MED:42998259",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42998259"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "openalex:2405|Parasitic Infections and Diagnostics",
      "subfield": "microbiology"
    }
  ],
  "title": "Molecular Detection and Characterization of Cryptosporidium spp. and Giardia duodenalis in Domestic Animals and Wildlife in Thailand With a Focus on Zoonotic Potential.",
  "title_text": "Molecular Detection and Characterization of Cryptosporidium spp. and Giardia duodenalis in Domestic Animals and Wildlife in Thailand With a Focus on Zoonotic Potential.",
  "type": "peer-reviewed",
  "updated_at": "2026-11-09T15:20:34Z",
  "url": "https://research.draconitic.com/paper/ewm2djhenvm6p",
  "venue": "Veterinary medicine and science",
  "versions": []
}