{
  "abstract": "Background/Objectives: Uracil-DNA glycosylase (UNG) initiates base excision repair by removing uracil from DNA. Although Family 1 UNGs are well characterized in model organisms, the biochemical and functional properties of Staphylococcus aureus UNG (SaUNG) is not well characterized. This study aimed to characterize SaUNG. Methods: Recombinant wild-type and mutant SaUNG proteins were examined using biochemical and kinetic assays, site-directed mutagenesis, genetic complementation in Escherichia coli, and in vitro reconstitution of S. aureus base excision repair. Results: SaUNG exhibited maximal activity at pH 8.0–8.5, 37–45 °C, and 100–150 mM KCl and lacked AP-lyase activity. Catalytic efficiencies (kcat/Km) were 1.39 × 109, 3.79 × 108, and 2.93 × 108 M−1·min−1 for ssU, U:G, and U:A, respectively, establishing the preference ssU > U:G > U:A. H180A retained residual activity, whereas simultaneous substitution of Asp59 and His180 abolished detectable activity. Complete inhibition confirmed conserved biochemical properties of Family 1 UNGs. SaUNG complemented the mutation avoidance defect of an E. coli (ung− mug−) mutant and initiated uracil repair in a reconstituted S. aureus base excision repair system. Conclusions: SaUNG is a canonical Family 1 UNG with experimentally characterized biochemical properties, catalytic determinants, and biological function in uracil repair.",
  "abstract_shown": true,
  "affiliations": [],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 9,
  "authors": [
    {
      "name": "Arina Koreneva"
    },
    {
      "name": "Albina Sugirova"
    },
    {
      "name": "Bakhtiyar Yakupov"
    },
    {
      "name": "Maria V Safonkina"
    },
    {
      "name": "Anton V Endutkin"
    },
    {
      "name": "Dmitry O Zharkov"
    },
    {
      "name": "Murat Saparbaev"
    },
    {
      "name": "Sailau Abeldenov"
    },
    {
      "name": "Aigerim Turgimbayeva"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/ewm6qzmcxdgxy.bib",
  "cited_by": 0,
  "doi": "10.3390/pathogens15101012",
  "fulltext_url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13708272/?tool=EBI",
  "id": "ewm6qzmcxdgxy",
  "issns": [
    "2076-0817"
  ],
  "landing_url": "https://hal.science/hal-05772353v1",
  "language": "en",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "hal",
      "0.sdv"
    ],
    [
      "hal",
      "1.sdv.bbm"
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      "1.sdv.mp"
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      "hal",
      "2.sdv.mp.bac"
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    [
      "openalex",
      "1312|DNA Repair Mechanisms"
    ]
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  "openalex": "W7214228456",
  "pdf_url": "https://hal.science/hal-05772353/document",
  "pmcid": "PMC13708272",
  "pmid": null,
  "published": "2026-09-24",
  "publisher": "MDPI",
  "ris": "https://research.draconitic.com/paper/ewm6qzmcxdgxy.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:17:44Z",
      "native_id": "hal-05772353",
      "source": "hal",
      "url": "https://hal.science/hal-05772353v1"
    },
    {
      "first_seen": "2026-10-30T12:21:51Z",
      "native_id": "PMC:PMC13708272",
      "source": "europepmc",
      "url": "https://europepmc.org/article/PMC/PMC13708272"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "hal:1.sdv.bbm",
      "subfield": "general"
    },
    {
      "field": "biology",
      "origin": "hal:1.sdv.mp",
      "subfield": "microbiology"
    }
  ],
  "title": "Functional Characterization of Uracil-DNA Glycosylase from Staphylococcus aureus",
  "title_text": "Functional Characterization of Uracil-DNA Glycosylase from Staphylococcus aureus",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:21:51Z",
  "url": "https://research.draconitic.com/paper/ewm6qzmcxdgxy",
  "venue": "Pathogens",
  "versions": []
}