{
  "abstract": "Background: Major depressive disorder (MDD) is associated with widespread molecular abnormalities in the brain; however, the contribution of ribosome-centered regulatory mechanisms remains poorly understood. Ribosomal RNA (rRNA) 2′-O-methylation (Nm) is the most abundant and evolutionarily conserved RNA modification and has emerged as a determinant of ribosome heterogeneity and translational control. However, the rRNA Nm landscape has not been examined in psychiatric illness. Here, we used RiboMeth-seq to map site-specific rRNA Nm in the MDD brain. Methods: RiboMeth-seq was performed in the dorsolateral prefrontal cortex of individuals with MDD ( n = 17) and matched nonpsychiatric control subjects ( n = 20) using an Illumina NextSeq2000 in 50-bp single-end mode. Specific RiboMeth-seq scores were calculated in ±2 nucleotide intervals for all RNA positions and extracted for the positions of known Nm residues in target RNA. Results: The overall distribution of ribose methylation scores was highly similar between groups, indicating the preservation of the global rRNA Nm landscape in MDD. At the same time, we identified 8 significantly altered Nm sites, including 4 in 28S rRNA (Am2802, Cm2848, Cm3866, and Gm4593) and 4 in 18S rRNA (Um172, Am576, Am1031, and Gm1490). These selective changes occurred in the absence of widespread remodeling, supporting a model in which MDD is associated with targeted modification of functionally relevant ribosomal positions rather than a generalized disruption of ribosome integrity. Conclusions: Our findings identify rRNA Nm as a previously unrecognized layer of molecular alteration in MDD and suggest that site-specific variation in ribosome composition may contribute to aberrant translational regulation in the depressed brain.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Psychiatry and Behavioral Neurobiology, Heersink School of Medicine, University of Alabama at Birmingham, Birmingham, Alabama",
    "Address correspondence to Yogesh Dwivedi, Ph.D."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 4,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Anuj K. Verma"
    },
    {
      "affs": [
        0
      ],
      "name": "Bhaskar Roy"
    },
    {
      "affs": [
        0
      ],
      "name": "Kevin Prall"
    },
    {
      "affs": [
        1
      ],
      "name": "Yogesh Dwivedi"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/excmvtdwb7nrf.bib",
  "cited_by": 0,
  "doi": "10.1016/j.bpsgos.2026.100832",
  "fulltext_url": "http://www.sciencedirect.com/science/article/pii/S266717432600145X",
  "id": "excmvtdwb7nrf",
  "issns": [
    "2667-1743"
  ],
  "landing_url": "https://doi.org/10.1016/j.bpsgos.2026.100832",
  "language": "en",
  "licence": "CC-BY-NC-ND",
  "licence_label": "CC BY-NC-ND",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "lcc",
      "RC435-571"
    ],
    [
      "openalex",
      "1312|RNA modifications and cancer"
    ]
  ],
  "openalex": "W7213973442",
  "pdf_url": "https://www.sciencedirect.com/science/article/pii/S266717432600145X/pdf",
  "pmcid": "PMC13743952",
  "pmid": "42999788",
  "published": "2026-09-22",
  "publisher": "Elsevier",
  "ris": "https://research.draconitic.com/paper/excmvtdwb7nrf.ris",
  "sources": [
    {
      "first_seen": "2026-11-01T14:21:07Z",
      "native_id": "0762bcb5cdbc4eb9a8d16ea1ba0532f0",
      "source": "doaj",
      "url": "https://doi.org/10.1016/j.bpsgos.2026.100832"
    },
    {
      "first_seen": "2026-11-09T15:20:41Z",
      "native_id": "MED:42999788",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42999788"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|RNA modifications and cancer",
      "subfield": "molecular-biology"
    }
  ],
  "title": "RiboMeth-Sequencing Reveals Selective Ribosomal RNA 2′-O-Methylation Changes in the Dorsolateral Prefrontal Cortex of Subjects With Major Depressive Disorder",
  "title_text": "RiboMeth-Sequencing Reveals Selective Ribosomal RNA 2′-O-Methylation Changes in the Dorsolateral Prefrontal Cortex of Subjects With Major Depressive Disorder",
  "type": "peer-reviewed",
  "updated_at": "2026-11-09T15:20:41Z",
  "url": "https://research.draconitic.com/paper/excmvtdwb7nrf",
  "venue": "Biological Psychiatry Global Open Science",
  "versions": []
}