{
  "abstract": "Rapid pathogen identification optimizes antibiotic therapy in bacteremia and can be achieved through molecular-based diagnostic tests. This study assesses the clinical impact after the implementation of an updated multiplex PCR panel (BIOFIRE Blood Culture Identification 2, BCID2). This single-center, retrospective, quasi-experimental study compared outcomes in adult patients with gram-negative bloodstream infections (GN-BSIs) pre and post BCID2 implementation. Data were collected from 1 March 2022 to 1 March 2024. Patients were included if they were ≥18 years of age, had a gram-negative organism identified by the panels, and received ≥48 h of antibiotics. The primary outcome was time to effective antimicrobial therapy; secondary outcomes included time to appropriate therapy, 30-day mortality, and hospital length of stay. A total of 180 patients were included (90 per BCID and BCID2 groups). Baseline characteristics were similar: median age 62 years, mostly non-critically ill, Charlson Comorbidity Index 4, and Pitt Bacteremia score 1 in both groups. Median time to effective therapy was reduced in the BCID2 vs BCID group (1 vs 4 h, P = 0.004) as well as time to appropriate therapy (25 vs 49 h, P = 0.627). In the ESBL subgroup, BCID2 significantly reduced time to effective therapy (0.5 vs 3 h, P = 0.006) and time to appropriate therapy (16 vs 44 h, P = 0.016). There was no significant difference in mortality (6.7% vs 8.9%, P = 0.756) or length of stay (10 vs 14 days, P = 0.134) between BCID and BCID2 groups. The implementation of BCID2 significantly reduced time to effective therapy in GN-BSIs, particularly among resistant organisms.\n\nIMPORTANCE: This study evaluated the impact of transitioning from BCID to BCID2 panel in gram-negative bloodstream infections to address gaps in resistance detection and assess whether expanded molecular diagnostics improve time to antibiotic therapy and clinical outcomes.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Clinical Pharmacy Services, Westchester Medical Center, Valhalla, New York, USA",
    "Department of Infectious Diseases Services, Westchester Medical Center, Valhalla, New York, USA",
    "Department of Pathology, Westchester Medical Center, Valhalla, New York, USA"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 6,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Rawan Hussein",
      "orcid": "0009-0007-0011-3928"
    },
    {
      "affs": [
        0
      ],
      "name": "Leslie Lee"
    },
    {
      "affs": [
        1
      ],
      "name": "Rajat Nog"
    },
    {
      "affs": [
        1
      ],
      "name": "Donald Chen"
    },
    {
      "affs": [
        2
      ],
      "name": "Vishnu Chaturvedi",
      "orcid": "0000-0002-3922-9676"
    },
    {
      "affs": [
        0
      ],
      "name": "Nicholas Feola",
      "orcid": "0000-0002-3352-9109"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/exn40ce61xxar.bib",
  "cited_by": 0,
  "doi": "10.1128/jcm.00328-26",
  "fulltext_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13712940/",
  "id": "exn40ce61xxar",
  "issns": [
    "0095-1137",
    "1098-660X"
  ],
  "landing_url": "https://doi.org/10.1128/jcm.00328-26",
  "language": "en",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Diagnostic Access and Equity"
    ],
    [
      "openalex",
      "1308|Bacterial Identification and Susceptibility Testing"
    ]
  ],
  "openalex": "W7204803442",
  "pdf_url": null,
  "pmcid": "PMC13712940",
  "pmid": "42671186",
  "published": "2026-08-31",
  "publisher": "American Society for Microbiology (ASM)",
  "ris": "https://research.draconitic.com/paper/exn40ce61xxar.ris",
  "sources": [
    {
      "first_seen": "2026-10-31T12:23:25Z",
      "native_id": "PMC13712940",
      "source": "pmc",
      "url": "https://doi.org/10.1128/jcm.00328-26"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:1308|Bacterial Identification and Susceptibility Testing",
      "subfield": "general"
    }
  ],
  "title": "Evaluation of time to antibiotic therapy after implementation of an updated rapid diagnostic test for gram-negative bacteremia",
  "title_text": "Evaluation of time to antibiotic therapy after implementation of an updated rapid diagnostic test for gram-negative bacteremia",
  "type": "peer-reviewed",
  "updated_at": "2026-10-31T12:23:25Z",
  "url": "https://research.draconitic.com/paper/exn40ce61xxar",
  "venue": "Journal of Clinical Microbiology",
  "versions": []
}