{
  "abstract": "Bacteriophage P22 ejects four internal proteins into infected cells to assemble a channel for genome translocation across the cell envelope. However, how these proteins cooperate to build the trans-envelope channel remains enigmatic. Using in situ single-particle cryogenic electron microscopy, we determined near-atomic in vivo structures of P22 infection intermediates, revealing how the tail hub protein gp10 templates the sequential assembly of three internal proteins gp7*, gp20 and gp16 into a ~50-nm trans-envelope channel that extends from the virion into the host cytoplasm. Functional analyses explain why phage DNA can be detected in supernatant fluids during infection by channel-defective mutants. Our structures also provide a mechanistic basis for the long-standing observation that a P22 virion lacking gp16 can be complemented, extra-cytoplasmically, by a co-infecting virion lacking gp20, thereby allowing the genome in the gp16-deficient particle to enter the host cytoplasm.",
  "abstract_shown": true,
  "affiliations": [
    "Yale University"
  ],
  "article_type": "New results",
  "arxiv": null,
  "author_total": 7,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "H. Yu"
    },
    {
      "name": "J. Yue"
    },
    {
      "name": "T. Park"
    },
    {
      "name": "c. wang"
    },
    {
      "name": "M. Chen"
    },
    {
      "name": "J. Liu"
    },
    {
      "name": "I. J. Molineux"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/ey1sna88c0b7a.bib",
  "cited_by": 0,
  "doi": "10.64898/2026.09.03.749273",
  "fulltext_url": "https://www.biorxiv.org/content/10.64898/2026.09.03.749273v1.full",
  "id": "ey1sna88c0b7a",
  "issns": [],
  "landing_url": "https://www.biorxiv.org/content/10.64898/2026.09.03.749273v1",
  "language": null,
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "biorxiv",
      "microbiology"
    ],
    [
      "openalex",
      "2303|Bacteriophages and microbial interactions"
    ]
  ],
  "openalex": "W7212030709",
  "pdf_url": "https://www.biorxiv.org/content/10.64898/2026.09.03.749273v1.full.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-09-03",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/ey1sna88c0b7a.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:20:33Z",
      "native_id": "10.64898/2026.09.03.749273",
      "source": "biorxiv",
      "url": "https://www.biorxiv.org/content/10.64898/2026.09.03.749273v1"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "biorxiv:microbiology",
      "subfield": "microbiology"
    }
  ],
  "title": "Sequential assembly in vivo of the bacteriophage P22 genome translocation channel",
  "title_text": "Sequential assembly in vivo of the bacteriophage P22 genome translocation channel",
  "type": "preprint",
  "updated_at": "2026-10-30T12:20:33Z",
  "url": "https://research.draconitic.com/paper/ey1sna88c0b7a",
  "venue": "bioRxiv",
  "versions": []
}