{
  "abstract": "Abnormal protein-protein interactions (PPIs) involving signaling proteins contribute to numerous human diseases. Targeting such aberrant PPIs with selective inhibitory molecules is a promising therapeutic strategy, yet assessing how many PPIs are affected by a candidate drug, and whether inhibition occurs before or after complex assembly, remains technically challenging. Here, we present a live-cell methodology that captures drug-sensitive PPIs by integrating drug-induced cellular responses with interaction mapping. As a proof of concept, we identify extracellular signal-regulated kinase 1 PPIs that are selectively altered by a novel chemical molecule exhibiting antitumor efficacy. We further apply complementary real-time imaging tools to visualize the dissociation of preassembled extracellular signal-regulated kinase 1-cofactor complexes in living cells. Our approach provides a generalizable framework for decoding drug activity on PPIs for virtually any protein target, enabling more precise and physiologically relevant evaluation of therapeutic mechanisms.",
  "abstract_shown": true,
  "affiliations": [],
  "article_type": null,
  "arxiv": null,
  "author_total": 16,
  "authors": [
    {
      "name": "Cindy Kundlacz"
    },
    {
      "name": "Christelle Forcet"
    },
    {
      "name": "Nawal Hajj Sleiman"
    },
    {
      "name": "Samantha Vonau"
    },
    {
      "name": "Frédéric Marmigère"
    },
    {
      "name": "Benjamin Gillet"
    },
    {
      "name": "Sandrine Hughes"
    },
    {
      "name": "Laurent Gilquin"
    },
    {
      "name": "Hélène Duplus"
    },
    {
      "name": "Agnès Dumont"
    },
    {
      "name": "Gaël Yvert"
    },
    {
      "name": "Françoise Bleicher"
    },
    {
      "name": "Arnaud Gautier"
    },
    {
      "name": "Isabelle Coste"
    },
    {
      "name": "Toufic Renno"
    },
    {
      "name": "Samir Merabet"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/ey8gw7gpxeyme.bib",
  "cited_by": 0,
  "doi": "10.1016/j.jbc.2026.113557",
  "fulltext_url": null,
  "id": "ey8gw7gpxeyme",
  "issns": [
    "0021-9258",
    "1083-351X"
  ],
  "landing_url": "https://cnrs.hal.science/hal-05772402v1",
  "language": "en",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "journal",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "hal",
      "0.sdv"
    ],
    [
      "openalex",
      "1312|Bioinformatics and Genomic Networks"
    ]
  ],
  "openalex": "W7213462687",
  "pdf_url": null,
  "pmcid": null,
  "pmid": "42749262",
  "published": "2026-09-17",
  "publisher": "American Society for Biochemistry and Molecular Biology",
  "ris": "https://research.draconitic.com/paper/ey8gw7gpxeyme.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:17:44Z",
      "native_id": "hal-05772402",
      "source": "hal",
      "url": "https://cnrs.hal.science/hal-05772402v1"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|Bioinformatics and Genomic Networks",
      "subfield": "molecular-biology"
    }
  ],
  "title": "A sensitive Interactome-based approach to characterize drug activity in living human cells",
  "title_text": "A sensitive Interactome-based approach to characterize drug activity in living human cells",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:17:44Z",
  "url": "https://research.draconitic.com/paper/ey8gw7gpxeyme",
  "venue": "Journal of Biological Chemistry",
  "versions": []
}