{
  "abstract": "Danshensu (DSS), a water-soluble bioactive compound with significant antioxidant, anti-inflammatory, cardioprotective and neuroprotective properties, is widely utilized in cardiovascular and cerebrovascular disease. In this study, we established a multi-enzyme cascade system in engineered Escherichia coli to synthesize DSS from L-Tyrosine. The initial co-expression of 4-hydroxyphenylacetate 3-monooxygenase (HpaBC), L-amino acid deaminase (aadL), glucose dehydrogenase (gdh) and D-lactate dehydrogenase (ldhD) yielded 5.1 mM DSS. The production of DSS increased to 6.53 mM through optimizing plasmid copy number. After optimizing the conditions, the yield of DSS reached 8.66 mM from strain 6. Finally, whole cell biotransformation using strain 6 in bioreactor yielded 19.47 mM DSS within 32h. These findings provide valuable insights for the scalable biosynthesis of pharmaceutically important compound of DSS from naturally available substrates of L-Tyrosine.",
  "abstract_shown": true,
  "affiliations": [
    "Henan Key Laboratory of Tea Plant Biology, College of Tea and Food Science, Xinyang Normal University, Xinyang, Henan, China.",
    "Dabie Mountain Laboratory, Xinyang, Henan, China.",
    "Henan International Joint Laboratory of Tea-oil tree Biology and High-Value Utilization, College of Tea and Food Science, Xinyang Normal University, Xinyang, Henan, China."
  ],
  "article_type": "Research Article",
  "arxiv": null,
  "author_total": 8,
  "authors": [
    {
      "affs": [
        0,
        1,
        2
      ],
      "name": "Tianzhen Xiong",
      "orcid": "0000-0001-6868-1269"
    },
    {
      "affs": [
        0,
        2
      ],
      "name": "Shuqing Li"
    },
    {
      "affs": [
        0,
        2
      ],
      "name": "Xiaoya Li"
    },
    {
      "affs": [
        0,
        2
      ],
      "name": "Xinmeng Li"
    },
    {
      "affs": [
        0,
        2
      ],
      "name": "Mengdan Wu"
    },
    {
      "affs": [
        0,
        2
      ],
      "name": "Jinru Liu"
    },
    {
      "affs": [
        0,
        2
      ],
      "name": "Jinxiu Guo"
    },
    {
      "affs": [
        0,
        2
      ],
      "name": "Xinrui Li"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/eypsmacgj95ex.bib",
  "cited_by": 0,
  "doi": "10.1371/journal.pone.0361484",
  "fulltext_url": "https://europepmc.org/articles/PMC13693876",
  "id": "eypsmacgj95ex",
  "issns": [
    "1932-6203"
  ],
  "landing_url": "https://doi.org/10.1371/journal.pone.0361484",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1312|Microbial Metabolic Engineering and Bioproduction"
    ],
    [
      "plos",
      "/Biology and life sciences/Biochemistry/Neurochemistry/Neurochemicals/Levodopa"
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      "/Biology and life sciences/Genetics/Gene expression"
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      "plos",
      "/Biology and life sciences/Microbiology/Medical microbiology/Microbial pathogens/Bacterial pathogens/Escherichia coli"
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    [
      "plos",
      "/Biology and life sciences/Molecular biology/Molecular biology techniques/DNA construction/Plasmid construction"
    ],
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      "/Biology and life sciences/Neuroscience/Neurochemistry/Neurochemicals/Levodopa"
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      "/Biology and life sciences/Organisms/Bacteria/Enterobacteriaceae/Escherichia/Escherichia coli"
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      "/Biology and life sciences/Organisms/Bacteria/Gut bacteria/Escherichia/Escherichia coli"
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      "plos",
      "/Engineering and technology/Bioengineering/Biotechnology/Genetic engineering"
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      "/Medicine and health sciences/Pathology and laboratory medicine/Pathogens/Microbial pathogens/Bacterial pathogens/Escherichia coli"
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      "/Medicine and health sciences/Pharmacology/Drugs/Levodopa"
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      "plos",
      "/Physical sciences/Chemistry/Chemical compounds/Organic compounds/Carbohydrates/Monosaccharides/Glucose"
    ],
    [
      "plos",
      "/Physical sciences/Chemistry/Organic chemistry/Organic compounds/Carbohydrates/Monosaccharides/Glucose"
    ],
    [
      "plos",
      "/Research and analysis methods/Animal studies/Experimental organism systems/Model organisms/Escherichia coli"
    ],
    [
      "plos",
      "/Research and analysis methods/Animal studies/Experimental organism systems/Prokaryotic models/Escherichia coli"
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    [
      "plos",
      "/Research and analysis methods/Chemical synthesis"
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      "plos",
      "/Research and analysis methods/Model organisms/Escherichia coli"
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    [
      "plos",
      "/Research and analysis methods/Molecular biology techniques/DNA construction/Plasmid construction"
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  "pdf_url": "https://europepmc.org/articles/PMC13693876?pdf=render",
  "pmcid": "PMC13693876",
  "pmid": "42912794",
  "published": "2026-10-23",
  "publisher": "Public Library of Science",
  "ris": "https://research.draconitic.com/paper/eypsmacgj95ex.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:17:17Z",
      "native_id": "10.1371/journal.pone.0361484",
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      "url": "https://doi.org/10.1371/journal.pone.0361484"
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    {
      "first_seen": "2026-10-30T12:20:10Z",
      "native_id": "MED:42912794",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42912794"
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  "subjects": [
    {
      "field": "biochemistry",
      "origin": "plos:gene expression",
      "subfield": "molecular-biology"
    },
    {
      "field": "biology",
      "origin": "plos:bioengineering",
      "subfield": "systems-synthetic"
    }
  ],
  "title": "Multienzyme cascade biotransformation of L-Tyrosine to Danshensu by engineered Escherichia coli",
  "title_text": "Multienzyme cascade biotransformation of L-Tyrosine to Danshensu by engineered Escherichia coli",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:20:10Z",
  "url": "https://research.draconitic.com/paper/eypsmacgj95ex",
  "venue": "PLOS One",
  "versions": []
}