{
  "abstract": "Entamoeba bovis is a common intestinal amoeba of ruminants, yet molecularly confirmed data from Europe remain limited. We investigated the occurrence, morphology and genetic variability of E. bovis in Italian domestic and wild ruminants and developed a rapid species-specific polymerase chain reaction (PCR) assay. Faecal samples from calves (n = 45), goats (n = 26), sheep (n = 11) and Italian red deer Cervus elaphus italicus (n = 25) were examined by sediment microscopy and iodine staining. Amoebic stages were detected in 57% of bovine, 61.5% of caprine, 100% of ovine and 56% of red deer samples. Across hosts, cysts were consistently uninucleate and displayed typical E. bovis morphology, including frequent chromatoid bodies and an iodophilic glycogen mass. Representative positives were sequenced at the 18S rRNA locus (∼800 bp), confirming E. bovis (98.6–99.8% identity; 100% query coverage). Maximum-likelihood phylogenetic analysis placed all Italian isolates within the E. bovis clade and indicated a shared lineage across domestic ruminants and red deer, with no segregation by host or ecological setting. A newly designed E. bovis-specific PCR (375 bp) consistently yielded a single amplicon in all sequenced positives, supporting its use for rapid screening without sequencing.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Veterinary Medical Sciences (DIMEVET), Alma Mater Studiorum University of Bologna, Bologna, Italy",
    "Research, Ecology and Environment Dimension (D.R.E.Am. Italia), Pistoia, Italy"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 6,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Filippo Maria Dini",
      "orcid": "0000-0001-7378-8650"
    },
    {
      "affs": [
        0
      ],
      "name": "Talita Bordoni"
    },
    {
      "affs": [
        0
      ],
      "name": "Chiara Belli"
    },
    {
      "affs": [
        1
      ],
      "name": "Enrica Bellinello"
    },
    {
      "affs": [
        0
      ],
      "name": "Aurora Lattanzi"
    },
    {
      "affs": [
        0
      ],
      "name": "Roberta Galuppi"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/eyxqv9264e96r.bib",
  "cited_by": 0,
  "doi": "10.1017/s0031182026102327",
  "fulltext_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13735557/",
  "id": "eyxqv9264e96r",
  "issns": [
    "0031-1820",
    "1469-8161"
  ],
  "landing_url": "https://doi.org/10.1017/s0031182026102327",
  "language": "en",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Research Article"
    ],
    [
      "openalex",
      "2725|Amoebic Infections and Treatments"
    ]
  ],
  "openalex": "W7165201486",
  "pdf_url": "https://www.cambridge.org/core/services/aop-cambridge-core/content/view/ABA2CFDC0F11AF7236C700E439E59931/S0031182026102327a.pdf/div-class-title-first-molecular-and-morphological-characterization-of-em-class-italic-entamoeba-bovis-em-in-domestic-and-wild-ruminants-in-italy-reveals-a-shared-lineage-across-host-species-and-ecological-settings-div.pdf",
  "pmcid": "PMC13735557",
  "pmid": "42311173",
  "published": "2026-06-18",
  "publisher": "Cambridge University Press",
  "ris": "https://research.draconitic.com/paper/eyxqv9264e96r.ris",
  "sources": [
    {
      "first_seen": "2026-11-09T12:21:13Z",
      "native_id": "PMC13735557",
      "source": "pmc",
      "url": "https://doi.org/10.1017/s0031182026102327"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2725|Amoebic Infections and Treatments",
      "subfield": "infectious-disease"
    }
  ],
  "title": "First molecular and morphological characterization of Entamoeba bovis in domestic and wild ruminants in Italy reveals a shared lineage across host species and ecological settings",
  "title_text": "First molecular and morphological characterization of Entamoeba bovis in domestic and wild ruminants in Italy reveals a shared lineage across host species and ecological settings",
  "type": "peer-reviewed",
  "updated_at": "2026-11-09T12:21:13Z",
  "url": "https://research.draconitic.com/paper/eyxqv9264e96r",
  "venue": "Parasitology",
  "versions": []
}