{
  "abstract": "Microfluidic paper-based analytical devices (μPADs) provide an attractive platform for diagnostics owing to their low cost, passive fluid transport, and ease of fabrication. However, integrating dual-sensing capabilities into a single paper device while maintaining a simple manufacturing process remains challenging. Here, we present a foldable dual-mode microfluidic paper-based analytical device (DM-μPAD) that integrates laser-induced graphene (LIG)-based electrochemical sensing with enzyme-mediated colorimetric detection. A straightforward laser-patterning strategy was used to fabricate LIG electrodes directly patterned onto the polyimide tape-covered cellulose paper via a one-step laser engraving process, forming a conductive graphene network that serves as an electrochemical sensing module. A screen-printing process was used to form hydrophobic perfluoropolyether barriers, which define capillary-driven microfluidic channels that guide sample transport between sensing regions. The folded architecture enhances fluid-electrode interaction and enables simultaneous electrochemical and colorimetric measurements within a single analytical platform without electrode transfer or complex assembly. This dual-readout technique provides cross-validation of results to enhance detection reliability. The proposed DM-μPAD demonstrated good selectivity against common nonspecific compounds, a wide linear detection range of 0.01 to 3.0 mM, and a low detection limit of 9.23 μM in the electrochemical mode, while displaying a dynamic detection range of 0.1 to 2.0 mM with a detection limit of 57.9 μM in the colorimetric mode. Furthermore, the platform maintained high reproducibility, mechanical flexibility, and environmental stability. This work highlights the potential of laser-induced graphene-based paper microfluidics as a versatile platform for developing portable, cost-effective, and multiplexed biosensing devices.",
  "abstract_shown": true,
  "affiliations": [
    "Academia Sinica , , Nankang , ,"
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 4,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Che-Liang Yeh"
    },
    {
      "affs": [
        0
      ],
      "name": "Syed Atif Ali",
      "orcid": "0000-0002-8779-547X"
    },
    {
      "affs": [
        0
      ],
      "name": "Wen-Ti Wu"
    },
    {
      "affs": [
        0
      ],
      "name": "Hsiung-Lin Tu",
      "orcid": "0000-0003-1125-1879"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/eyymt7pdpy5gj.bib",
  "cited_by": 0,
  "doi": "10.1021/acsomega.6c09156",
  "fulltext_url": null,
  "id": "eyymt7pdpy5gj",
  "issns": [
    "2470-1343"
  ],
  "landing_url": "https://pubs.acs.org/acsodf/article/doi/10.1021/acsomega.6c09156/5457744/Laser-Induced-Graphene-Paper-Based-Microfluidic",
  "language": null,
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "2204|Biosensors and Analytical Detection"
    ]
  ],
  "openalex": "W7223461169",
  "pdf_url": "https://pubs.acs.org/doi/pdf/10.1021/acsomega.6c09156",
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-24",
  "publisher": "American Chemical Society (ACS)",
  "ris": "https://research.draconitic.com/paper/eyymt7pdpy5gj.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:18:40Z",
      "native_id": "10.1021/acsomega.6c09156",
      "source": "crossref",
      "url": "https://pubs.acs.org/acsodf/article/doi/10.1021/acsomega.6c09156/5457744/Laser-Induced-Graphene-Paper-Based-Microfluidic"
    }
  ],
  "subjects": [
    {
      "field": "chemistry",
      "origin": "issn:2470-1343",
      "subfield": "general"
    }
  ],
  "title": "Laser-Induced Graphene Paper-Based Microfluidic Platform for Dual-Mode Electrochemical and Colorimetric Detection of Glucose",
  "title_text": "Laser-Induced Graphene Paper-Based Microfluidic Platform for Dual-Mode Electrochemical and Colorimetric Detection of Glucose",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:18:40Z",
  "url": "https://research.draconitic.com/paper/eyymt7pdpy5gj",
  "venue": "ACS Omega",
  "versions": []
}