{ "abstract": "Yersinia pseudotuberculosis is a food-borne pathogen responsible for a self-limiting mesenteric lymphadenitis. Yersinia pseudotuberculosis lineages 1 and 8 are also associated to the Far East scarlet-like fever (FESLF) syndrome, characterized by skin rash, hyperemic tongue, and desquamation. Genome sequencing of lineage 8 FESLF strains previously revealed the presence in the plasmid pVM82 of dot/icm genes, homologous to those known to encode a Type 4B Secretion System (T4BSS) in the intracellular pathogens Legionella pneumophila and Coxiella burnetii . We validated the presence of all essential dot/icm genes required for the structure of a T4BSS and we identified acidic pH and 37 • C as the main signals triggering y T4BSS expression. Putative y T4BSS effectors were also identified using the S4TE algorithm but their secretion was not confirmed in the studied conditions. The y T4BSS is also expressed in epithelial cells and macrophages as well as in vivo during mouse infection, but it is not required for bacterial intracellular survival. Nevertheless, the y T4BSS is implicated in Y. pseudotuberculosis FESLF pathogenicity in mice when bacteria are orally inoculated. The y T4BSS does not influence organ colonization and is rather implicated in the induction of important necrosis lesions in mesenteric lymph nodes and ceca of mice. Cytokine profile analyses in mouse bone marrow-derived macrophages revealed a y T4BSS-dependent induction of innate immunity related cytokines and chemokines. In conclusion, we identify the y T4BSS as a virulence factor of Y. pseudotuberculosis strains from lineage 8 responsible for FESLF which modulates cytokine responses of the host innate immune system.", "abstract_shown": true, "affiliations": [ "Yersinia Research Unit, Institut Pasteur, Université Paris Cité, CNRS UMR 6047, F-75015 Paris, France.", "Yersinia National Reference Laboratory, WHO Collaborating Research & Reference Centre for Plague FRA-146, Institut Pasteur, Université Paris Cité, F-75015 Paris, France.", "United Kingdom Health Security Agency (UKHSA), Porton Down, SP4 0JG Salisbury, United Kingdom.", "Microbial Evolutionary Genomics, Institut Pasteur, Université Paris Cité, CNRS UMR 3525, F-75015 Paris, France.", "Environment and Infectious Risks Unit, Laboratory for Urgent Response to Biological Threats, Institut Pasteur, Université Paris Cité, F-75015 Paris, France.", "IBiSA Electron Microscopy Platform, INSERM U1259, Université de Tours et CHRU de Tours, F-37032 Tours, France.", "Bioinformatics and Biostatistics Hub, Institut Pasteur, Université Paris Cité, F-75015 Paris, France.", "Department of Anatomy and Comparative Pathology and Toxicology, Pathology and Immunology Group (UCO-PIG), UIC Zoonosis y Enfermedades Emergentes ENZOEM, International Excellence Agrifood Campus 'CeiA3', University of Córdoba, 14014 Córdoba, Spain." ], "article_type": "research article", "arxiv": null, "author_total": 16, "authors": [ { "affs": [ 0 ], "name": "Marion Lemarignier", "orcid": "0000-0003-4025-610X" }, { "affs": [ 0, 1 ], "name": "Cyril Savin", "orcid": "0000-0003-2659-3433" }, { "affs": [ 2 ], "name": "Inés Ruedas-Torres", "orcid": "0000-0002-5851-1888" }, { "affs": [ 0 ], "name": "Anne Derbise", "orcid": "0000-0001-7678-2190" }, { "affs": [ 3 ], "name": "Charles Coluzzi", "orcid": "0000-0003-2238-0836" }, { "affs": [ 4 ], "name": "Jose Pablo Marin-Obando", "orcid": "0000-0001-7843-8414" }, { "affs": [ 0 ], "name": "Josue Barquero-Chavarria", "orcid": "0000-0001-5588-8631" }, { "affs": [ 0 ], "name": "Pierre Lê-Bury", "orcid": "0000-0002-6010-2952" }, { "affs": [ 5 ], "name": "Julien Burlaud-Gaillard" }, { "affs": [ 0, 1 ], "name": "Julien Madej" }, { "affs": [ 0, 1 ], "name": "Rémi Beau" }, { "affs": [ 5 ], "name": "Philippe Roingeard", "orcid": "0000-0001-9131-3341" }, { "affs": [ 6 ], "name": "Pierre Lechat", "orcid": "0000-0003-1050-5582" }, { "affs": [ 3 ], "name": "Eduardo P C Rocha", "orcid": "0000-0001-7704-822X" }, { "affs": [ 7 ], "name": "Jaime Gómez-Laguna", "orcid": "0000-0002-1787-5642" }, { "affs": [ 0, 1 ], "name": "Javier Pizarro-Cerdá", "orcid": "0000-0002-4343-0508" } ], "bibtex": "https://research.draconitic.com/paper/ezj401eymghqq.bib", "cited_by": 0, "doi": "10.1093/femsml/uqag036", "fulltext_url": "https://europepmc.org/articles/PMC13664640", "id": "ezj401eymghqq", "issns": [ "2633-6693" ], "landing_url": "https://pasteur.hal.science/pasteur-05800334v1", "language": "en", "licence": "CC-BY-NC-4.0", "licence_label": "CC BY-NC 4.0", "licence_source": "article", "licence_url": "https://creativecommons.org/licenses/by-nc/4.0/", "metadata_licence": null, "native_subjects": [ [ "hal", "0.sdv" ], [ "hal", "1.sdv.mp" ], [ "hal", "2.sdv.mp.bac" ], [ "openalex", "1311|Yersinia bacterium, plague, ectoparasites research" ] ], "openalex": "W7214072532", "pdf_url": "https://pasteur.hal.science/pasteur-05800334/document", "pmcid": "PMC13664640", "pmid": "42877772", "published": "2026-09-23", "publisher": "Oxford University Press", "ris": "https://research.draconitic.com/paper/ezj401eymghqq.ris", "sources": [ { "first_seen": "2026-10-30T12:17:57Z", "native_id": "pasteur-05800334", "source": "hal", "url": "https://pasteur.hal.science/pasteur-05800334v1" }, { "first_seen": "2026-10-30T12:19:16Z", "native_id": "MED:42877772", "source": "europepmc", "url": "https://europepmc.org/article/MED/42877772" } ], "subjects": [ { "field": "biology", "origin": "hal:1.sdv.mp", "subfield": "microbiology" } ], "title": "A putative type IVB secretion system contributes to the pathogenicity of Yersinia pseudotuberculosis lineage 8 strains associated with the Far East scarlet-like fever", "title_text": "A putative type IVB secretion system contributes to the pathogenicity of Yersinia pseudotuberculosis lineage 8 strains associated with the Far East scarlet-like fever", "type": "peer-reviewed", "updated_at": "2026-10-30T12:19:16Z", "url": "https://research.draconitic.com/paper/ezj401eymghqq", "venue": "microLife", "versions": [] }