{
  "abstract": "Polymer modification of antibodies has attracted attention for enhancing antibody functionality. Modification with polyethylene glycol (PEG) or biopolymers such as antibody-oligonucleotide conjugates has been investigated. Although direct effects at the modification site have been understood, distal effects of modifications on intrinsic antibody functions such as antigen-binding and Fc receptor-binding activities remain poorly characterized. In this study, we prepared site-specifically PEGylated trastuzumab to evaluate the effects of the modification site and the size of PEG chains on antibody functions. Reporter gene assays revealed a significant reduction in antibody-dependent cell cytotoxicity (ADCC) activity in a site- and size-dependent manner. Both the reduction in interaction with the antigen and Fc{gamma}RIIIa were observed in a size-dependent manner. Interestingly, the effect of the modification site was only observed for ADCC activity, which suggests that the higher-order structure of the modified antibody plays a critical role in effector function. These results provide molecular design guidelines for the polymer modification of antibodies.",
  "abstract_shown": true,
  "affiliations": [
    "Kyoto University"
  ],
  "article_type": "New results",
  "arxiv": null,
  "author_total": 3,
  "authors": [
    {
      "name": "Y. Tsutsui"
    },
    {
      "name": "H. Ohno"
    },
    {
      "affs": [
        0
      ],
      "name": "H. Akiba"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/f1553qbsxjbs8.bib",
  "cited_by": 0,
  "doi": "10.64898/2026.09.23.753936",
  "fulltext_url": "https://www.biorxiv.org/content/10.64898/2026.09.23.753936v1.full",
  "id": "f1553qbsxjbs8",
  "issns": [],
  "landing_url": "https://www.biorxiv.org/content/10.64898/2026.09.23.753936v1",
  "language": null,
  "licence": "CC-BY-NC-ND",
  "licence_label": "CC BY-NC-ND",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "biorxiv",
      "biochemistry"
    ],
    [
      "openalex",
      "2741|Monoclonal and Polyclonal Antibodies Research"
    ]
  ],
  "openalex": "W7214168587",
  "pdf_url": "https://www.biorxiv.org/content/10.64898/2026.09.23.753936v1.full.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-09-23",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/f1553qbsxjbs8.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:24:27Z",
      "native_id": "10.64898/2026.09.23.753936",
      "source": "biorxiv",
      "url": "https://www.biorxiv.org/content/10.64898/2026.09.23.753936v1"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2741|Monoclonal and Polyclonal Antibodies Research",
      "subfield": "imaging"
    }
  ],
  "title": "Size-dependent effect of site-specific polyethylene glycol modification of trastuzumab on the antibody functions",
  "title_text": "Size-dependent effect of site-specific polyethylene glycol modification of trastuzumab on the antibody functions",
  "type": "preprint",
  "updated_at": "2026-10-30T12:24:27Z",
  "url": "https://research.draconitic.com/paper/f1553qbsxjbs8",
  "venue": "bioRxiv",
  "versions": []
}