{
  "abstract": "Extracellular vesicles (EVs) serve as key facilitators mediating intercellular communication in skeletal muscle, but the upstream mechanisms regulating their secretion remain incompletely understood. Using proteomic screening, we identified CXCL12 as a candidate HSF1-associated downstream factor. Functional assays showed that CXCL12 promoted C2C12 myoblast proliferation, restrained myogenic differentiation in a stage-dependent manner, and enhanced sEV secretion, accompanied by altered expression of early endosome- and MVB-associated markers. Functionally, CXCL12 knockdown attenuated the HSF1-induced increase in sEV secretion and the upregulation of EEA1 and CD63 protein levels, whereas exogenous recombinant CXCL12 partially restored EEA1 and CD63 expression in the skeletal muscle of muscle-specific HSF1 knockout mice. Collectively, these results are consistent with a model in which CXCL12 is associated with sEV secretion in coordination with changes in early endosome- and MVB-associated protein abundance, suggesting a previously underappreciated role for CXCL12 in vesicle trafficking in the skeletal muscle microenvironment.",
  "abstract_shown": true,
  "affiliations": [
    "Shanxi Key Laboratory of Animal Disease Research, Prevention and Control, College of Veterinary Medicine, Shanxi Agricultural University, Jinzhong 030801, China"
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 8,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Yajie Dong"
    },
    {
      "affs": [
        0
      ],
      "name": "Xiaoyuan Cao"
    },
    {
      "affs": [
        0
      ],
      "name": "Wenjuan Xie"
    },
    {
      "affs": [
        0
      ],
      "name": "Xuepeng Fan"
    },
    {
      "affs": [
        0
      ],
      "name": "Xiaomao Luo",
      "orcid": "0000-0001-8563-8211"
    },
    {
      "affs": [
        0
      ],
      "name": "Yi Yan"
    },
    {
      "affs": [
        0
      ],
      "name": "Jiayin Lu"
    },
    {
      "affs": [
        0
      ],
      "name": "Haidong Wang"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/f46m3ee504j2a.bib",
  "cited_by": 0,
  "doi": "10.3390/biom16111597",
  "fulltext_url": null,
  "id": "f46m3ee504j2a",
  "issns": [
    "2218-273X"
  ],
  "landing_url": "https://www.mdpi.com/2218-273X/16/11/1597",
  "language": null,
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [],
  "openalex": null,
  "pdf_url": null,
  "pmcid": null,
  "pmid": null,
  "published": "2026-11-02",
  "publisher": "MDPI AG",
  "ris": "https://research.draconitic.com/paper/f46m3ee504j2a.ris",
  "sources": [
    {
      "first_seen": "2026-11-03T00:23:55Z",
      "native_id": "10.3390/biom16111597",
      "source": "crossref",
      "url": "https://www.mdpi.com/2218-273X/16/11/1597"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "issn:2218-273X",
      "subfield": "general"
    }
  ],
  "title": "Chemokine CXCL12 Mediates Transcription Factor HSF1-Dependent Small Extracellular Vesicle Secretion Through Different Endosomal Stages",
  "title_text": "Chemokine CXCL12 Mediates Transcription Factor HSF1-Dependent Small Extracellular Vesicle Secretion Through Different Endosomal Stages",
  "type": "peer-reviewed",
  "updated_at": "2026-11-04T00:24:50Z",
  "url": "https://research.draconitic.com/paper/f46m3ee504j2a",
  "venue": "Biomolecules",
  "versions": []
}