{
  "abstract": "Background: Sucralose is widely used as a non-nutritive sweetener, yet its systemic consequences of long-term exposure remain incompletely understood. This study investigated the effects of long-term sucralose consumption on metabolic homeostasis and neurobehavioral outcomes. Methods: Male C57BL/6J mice were treated with 5 mg/kg body weight sucralose under non-stressed and unpredictable chronic mild stress conditions for 14 weeks. Results: Sucralose altered hepatic cholesterol regulation without significantly increasing serum triglyceride or total cholesterol relative to water controls. Significant treatment–stress interactions were detected for hepatic Srb1 (F = 6.511, p = 0.004) and intestinal Npc1l1 (F = 3.763, p = 0.032), indicating stress-dependent modulation of cholesterol absorption. Hepatic inflammation also showed significant interactions for Il6 (p < 0.001) and Tnfa (p = 0.003). In addition, the sucralose group exhibited heightened neuroinflammation, cerebral endoplasmic reticulum stress, and increased depressive-like behaviors. 16S rRNA sequencing demonstrated reduced α-diversity and a shift in microbial composition and predicted carbohydrate processing and transport functions. Spearman correlation analysis linked microbial, intestinal, hepatic, neuroinflammatory, and behavioral variables within sucralose-treated mice. Conclusions: Long-term sucralose consumption altered gut microbial composition, hepatic cholesterol regulation, and neuroinflammatory and behavioral outcomes.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Food and Nutrition, Chungnam National University, Daejeon 34134, Republic of Korea",
    "Glocal Lifecare Convergence Program, Interdisciplinary Education Center for the Innovative Next Generation Leaders in Glocal Lifecare, Chungnam National University, Daehak-ro 99, Yuseong-gu, Daejeon 34134, Republic of Korea",
    "Research Center for Microbiome-Brain Disorders, Chungnam National University, Daejeon 34134, Republic of Korea"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 5,
  "authors": [
    {
      "affs": [
        0,
        1
      ],
      "name": "Beulah Favour Ortutu",
      "orcid": "0000-0002-6084-7700"
    },
    {
      "affs": [
        0
      ],
      "name": "Kyeong-sook Keum"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Jin Gyeom Kim",
      "orcid": "0009-0001-6099-3273"
    },
    {
      "affs": [
        2
      ],
      "name": "Su-In Yoon",
      "orcid": "0000-0002-8633-9675"
    },
    {
      "affs": [
        0
      ],
      "name": "Jin Ah Cho",
      "orcid": "0000-0001-9265-2409"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/f4d8a05jvh8nh.bib",
  "cited_by": 0,
  "doi": "10.3390/nu18203452",
  "fulltext_url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13708054/?tool=EBI",
  "id": "f4d8a05jvh8nh",
  "issns": [
    "2072-6643"
  ],
  "landing_url": "https://www.mdpi.com/2072-6643/18/20/3452",
  "language": "eng",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "2916|Biochemical Analysis and Sensing Techniques"
    ]
  ],
  "openalex": "W7223145384",
  "pdf_url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13708054/pdf/?tool=EBI",
  "pmcid": "PMC13708054",
  "pmid": null,
  "published": "2026-10-01",
  "publisher": "MDPI AG",
  "ris": "https://research.draconitic.com/paper/f4d8a05jvh8nh.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:19:56Z",
      "native_id": "10.3390/nu18203452",
      "source": "crossref",
      "url": "https://www.mdpi.com/2072-6643/18/20/3452"
    },
    {
      "first_seen": "2026-10-30T12:21:44Z",
      "native_id": "PMC:PMC13708054",
      "source": "europepmc",
      "url": "https://europepmc.org/article/PMC/PMC13708054"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "issn:2072-6643",
      "subfield": "nutrition"
    }
  ],
  "title": "Long-Term Sucralose Consumption Alters Gut Microbiota and Hepatic Lipid Metabolism and Increases Depressive-like Behavior in Young Mice",
  "title_text": "Long-Term Sucralose Consumption Alters Gut Microbiota and Hepatic Lipid Metabolism and Increases Depressive-like Behavior in Young Mice",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:21:44Z",
  "url": "https://research.draconitic.com/paper/f4d8a05jvh8nh",
  "venue": "Nutrients",
  "versions": []
}