{
  "abstract": "Background: Chronic cerebral hypoperfusion (CCH) is a neurological disorder characterized by persistent insufficiency of cerebral blood flow, yet specific biomarkers and mechanistic insights are lacking.\n\nMethods: This study integrated transcriptomic and proteomic multi-omics analyses, along with behavioral assessments and immunofluorescence validation in a bilateral common carotid artery stenosis (BCAS) mouse model, to identify and validate candidate multi-omics biomarkers of CCH and explore their potential druggability. Leveraging public transcriptomic datasets and in-house proteomic data, differential expression analysis, protein-protein interaction network construction, and five topological algorithms identified Tlr2 , Anxa5 , and Grin2a as candidate biomarkers.\n\nResults: BCAS mice exhibited spatial memory impairments in behavioral tests. Immunofluorescence revealed upregulated TLR2 and ANXA5 , downregulated GRIN2A, and increased TLR2-IBA1 co-localization indicating microglial activation. Tlr2 and Anxa5 positively correlated with infiltrating immune cells, whereas Grin2a showed a negative correlation. A putative ceRNA network and in silico molecular docking suggested strong binding of pirinixic acid to both TLR2 and ANXA5.\n\nDiscussion: Tlr2 , Anxa5 , and Grin2a can serve as candidate multi-omics signature molecules of CCH in the mouse brain, with consistent alterations at the transcriptomic, proteomic, and tissue levels.",
  "abstract_shown": true,
  "affiliations": [
    "School of Medicine, University of Electronic Science and Technology of China , ,",
    "Department of Neurology, Sichuan Provincial People's Hospital , ,"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 9,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Xiang Fan"
    },
    {
      "affs": [
        1
      ],
      "name": "Meng-Yu Zhong"
    },
    {
      "affs": [
        1
      ],
      "name": "Huan Liu"
    },
    {
      "affs": [
        0
      ],
      "name": "Hao Tao"
    },
    {
      "affs": [
        0
      ],
      "name": "Yi Li"
    },
    {
      "affs": [
        1
      ],
      "name": "Xun Wu"
    },
    {
      "affs": [
        1
      ],
      "name": "Ying-jie Li"
    },
    {
      "affs": [
        0
      ],
      "name": "Bing-Hu Li"
    },
    {
      "affs": [
        0
      ],
      "name": "Neng-Wei Yu"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/f4tf46g1b1zr9.bib",
  "cited_by": 0,
  "doi": "10.3389/fneur.2026.1911864",
  "fulltext_url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13668749/?tool=EBI",
  "id": "f4tf46g1b1zr9",
  "issns": [
    "1664-2295"
  ],
  "landing_url": "https://europepmc.org/article/PMC/PMC13668749",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "2808|Neurological Disease Mechanisms and Treatments"
    ]
  ],
  "openalex": "W7215111771",
  "pdf_url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13668749/pdf/?tool=EBI",
  "pmcid": "PMC13668749",
  "pmid": null,
  "published": "2026-10-01",
  "publisher": "Frontiers Media SA",
  "ris": "https://research.draconitic.com/paper/f4tf46g1b1zr9.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:18:18Z",
      "native_id": "PMC:PMC13668749",
      "source": "europepmc",
      "url": "https://europepmc.org/article/PMC/PMC13668749"
    },
    {
      "first_seen": "2026-10-30T12:20:16Z",
      "native_id": "10.3389/fneur.2026.1911864",
      "source": "crossref",
      "url": "https://www.frontiersin.org/articles/10.3389/fneur.2026.1911864/full"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2808|Neurological Disease Mechanisms and Treatments",
      "subfield": "neurology"
    }
  ],
  "title": "Cross-dataset transcriptomics–proteomics analysis identifies Tlr2, Anxa5, and Grin2a as candidate mouse-brain signatures associated with chronic cerebral hypoperfusion",
  "title_text": "Cross-dataset transcriptomics–proteomics analysis identifies Tlr2, Anxa5, and Grin2a as candidate mouse-brain signatures associated with chronic cerebral hypoperfusion",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:20:16Z",
  "url": "https://research.draconitic.com/paper/f4tf46g1b1zr9",
  "venue": "Frontiers in neurology",
  "versions": []
}