{
  "abstract": "Meso2EM is a correlative light and electron microscopy workflow that transfers neurons selected from mesoscale functional images to targeted electron microscopy. We recorded Ca{superscript 2} signals from layer 2/3 neurons across a contiguous 3 x 3 mm cortical field in awake mice and reidentified a selected neuron after fixation and tangential sectioning. Lectin-labeled vascular architecture served as a shared landmark across in vivo two-photon imaging, confocal microscopy, laboratory micro-CT of resin-embedded tissue, and block-surface scanning electron microscopy, guiding focused-ion-beam scanning electron microscopy to the target cell body. The same progressive-targeting principle also supported serial ATUM-SEM reconstruction of an in vivo-tracked dendrite and serial transmission electron microscopy of optically selected dendrites from a patch-clamp-recorded Martinotti cell. Meso2EM therefore provides a practical route for preserving target identity across large changes in scale and specimen state while restricting electron-microscopy acquisition to a selected region.",
  "abstract_shown": true,
  "affiliations": [
    "National Institute for Physiological Sciences"
  ],
  "article_type": "New results",
  "arxiv": null,
  "author_total": 11,
  "authors": [
    {
      "name": "I. Oomoto"
    },
    {
      "name": "M. Murate"
    },
    {
      "name": "J. Sohn"
    },
    {
      "name": "M. Tamura"
    },
    {
      "name": "S. Hatada"
    },
    {
      "name": "N. Egawa"
    },
    {
      "name": "M. Odagawa"
    },
    {
      "name": "M. Suga"
    },
    {
      "name": "Y. Kawaguchi"
    },
    {
      "name": "M. Murayama"
    },
    {
      "affs": [
        0
      ],
      "name": "Y. Kubota"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/f5zwsgqs7v5ss.bib",
  "cited_by": 0,
  "doi": "10.64898/2026.08.25.746890",
  "fulltext_url": "https://www.biorxiv.org/content/10.64898/2026.08.25.746890v1.full",
  "id": "f5zwsgqs7v5ss",
  "issns": [],
  "landing_url": "https://www.biorxiv.org/content/10.64898/2026.08.25.746890v1",
  "language": null,
  "licence": "CC-BY-NC-ND",
  "licence_label": "CC BY-NC-ND",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "biorxiv",
      "neuroscience"
    ],
    [
      "openalex",
      "1315|Advanced Electron Microscopy Techniques and Applications"
    ]
  ],
  "openalex": "W7204627568",
  "pdf_url": "https://www.biorxiv.org/content/10.64898/2026.08.25.746890v1.full.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-08-25",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/f5zwsgqs7v5ss.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:18:06Z",
      "native_id": "10.64898/2026.08.25.746890",
      "source": "biorxiv",
      "url": "https://www.biorxiv.org/content/10.64898/2026.08.25.746890v1"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "biorxiv:neuroscience",
      "subfield": "neuroscience"
    }
  ],
  "title": "Meso2EM: a cross-scale CLEM workflow linking mesoscale functional imaging to targeted electron microscopy",
  "title_text": "Meso2EM: a cross-scale CLEM workflow linking mesoscale functional imaging to targeted electron microscopy",
  "type": "preprint",
  "updated_at": "2026-10-30T12:18:06Z",
  "url": "https://research.draconitic.com/paper/f5zwsgqs7v5ss",
  "venue": "bioRxiv",
  "versions": []
}