{
  "abstract": "Objective: The current study aimed to investigate whether miR-27b-3p regulates oxidative stress and inflammatory responses through the Nrf2/HO-1 signaling pathway during the progression of fatty liver. Liver tissues from dairy cows suffering from fatty liver and high-fat diet (HFD)-induced fatty liver mice were gathered to assess lipid accumulation, oxidative stress, inflammation, and the expression of miR-27b-3p and components of the Nrf2/HO-1 pathway.\n\nMethods: Liver tissues from dairy cows with fatty liver and HFD-induced fatty liver mice were collected to evaluate lipid accumulation, oxidative stress, inflammation, and the expression of miR-27b-3p and components of the Nrf2/HO-1 pathway. An in vitro steatosis model was established in AML-12 hepatocytes using palmitic acid (PA). Oxidative stress markers (reactive oxygen species [ROS], superoxide dismutase [SOD], and malondialdehyde [MDA]), inflammatory cytokines (IL-1β, IL-6, and TNF-α), and proteins associated with apoptosis were assessed. Bioinformatic analysis and dual-luciferase reporter assays were used to determine the interaction between miR-27b-3p and Nrf2. Functional assays were performed using miR-27b-3p mimetics and antagonists to evaluate its regulatory effects on the Nrf2/HO-1 signaling pathway and downstream cellular responses.\n\nResults: Fatty liver tissues from dairy cows and HFD-fed mice exhibited significant lipid deposition, enhanced oxidative stress, and elevated inflammatory responses, accompanied by upregulation of miR-27b-3p expression and reduced Nrf2/HO-1 signaling activity. Comparable alterations were observed in PA-treated AML-12 cells. Bioinformatic analysis and dual-luciferase experiments validated that miR-27b-3p directly binds to the 3′ untranslated region of Nrf2. Inhibition of miR-27b-3p restored Nrf2 and HO-1 expression, reduced ROS and MDA levels, enhanced SOD activity, suppressed NF-κB activation, decreased pro-inflammatory cytokine production, and alleviated apoptosis. Conversely, the overexpression of miR-27b-3p further inhibited Nrf2/HO-1 signaling and exacerbated oxidative stress, inflammation, and cellular damage.\n\nConclusion: miR-27b-3p accelerates the progression of fatty liver toward steatohepatitis by directly inhibiting Nrf2 and downregulating the Nrf2/HO-1 antioxidant pathway, which increase oxidative stress, inflammatory responses, and hepatocyte apoptosis. These findings identify miR-27b-3p as a key upstream regulator of redox imbalance in fatty liver and suggest that targeting miR-27b-3p could be a promising strategy for the prevention and treatment of bovine fatty liver disease.",
  "abstract_shown": true,
  "affiliations": [
    "College of Veterinary Medicine, Yunnan Agricultural University, Kunming, China"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 5,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Kui Wang",
      "orcid": "0009-0003-4844-7258"
    },
    {
      "affs": [
        0
      ],
      "name": "Hao Pan",
      "orcid": "0009-0005-0081-0921"
    },
    {
      "affs": [
        0
      ],
      "name": "Yan Tian",
      "orcid": "0009-0004-5205-1149"
    },
    {
      "affs": [
        0
      ],
      "name": "Kangfeng Jiang",
      "orcid": "0000-0001-9248-0723"
    },
    {
      "affs": [
        0
      ],
      "name": "Xiaobing Li",
      "orcid": "0000-0001-9105-6365"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/f6rhtqh2qve15.bib",
  "cited_by": 0,
  "doi": "10.5713/ab.260476",
  "fulltext_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13740664/",
  "id": "f6rhtqh2qve15",
  "issns": [
    "2765-0189",
    "2765-0235"
  ],
  "landing_url": "https://doi.org/10.5713/ab.260476",
  "language": "en",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Animal Health"
    ],
    [
      "heading",
      "Article"
    ],
    [
      "openalex",
      "1306|MicroRNA in disease regulation"
    ]
  ],
  "openalex": "W7171418783",
  "pdf_url": "https://www.animbiosci.org/upload/pdf/ab-260476.pdf",
  "pmcid": "PMC13740664",
  "pmid": "42526872",
  "published": "2026-07-27",
  "publisher": "Asian-Australasian Association of Animal Production Societies (AAAP)",
  "ris": "https://research.draconitic.com/paper/f6rhtqh2qve15.ris",
  "sources": [
    {
      "first_seen": "2026-11-10T12:21:52Z",
      "native_id": "PMC13740664",
      "source": "pmc",
      "url": "https://doi.org/10.5713/ab.260476"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:1306|MicroRNA in disease regulation",
      "subfield": "oncology"
    }
  ],
  "title": "miR-27b-3p aggravates steatohepatitis progression by targeting the Nrf2/HO-1 pathway",
  "title_text": "miR-27b-3p aggravates steatohepatitis progression by targeting the Nrf2/HO-1 pathway",
  "type": "peer-reviewed",
  "updated_at": "2026-11-10T12:21:52Z",
  "url": "https://research.draconitic.com/paper/f6rhtqh2qve15",
  "venue": "Animal Bioscience",
  "versions": []
}