{
  "abstract": "Microbial endophytes have emerged as a potential source of bioactive secondary metabolites and provide an excellent resource to overcome the challenges resulting from over-consumption of medicinal plants. Ammi visnaga is an Egyptian medicinal plant widely recognized for its medicinal value. The roots of A. visnaga are considered as waste products; the aim of this study is to explore the fungal endophytes associated with A.visnaga roots. Fungal isolate was identified using PCR; large-scale fermentation was performed on solid rice media followed by extraction with Ethyl acetate. The fungal extract was subjected to chromatographic purification utilizing VLC and HPLC. Isolated compounds were identified using HRESIMS and NMR techniques. UPLC-ESI-Q-Tof was utilized to perform the chemical profiling of the fungal extract. Disc diffusion and MIC experiments were performed to evaluate the antimicrobial activity. In silico assessment was performed using AutoDock Vina against ten bacterial proteins incorporated in DNA replication, antibiotic resistance, and virulence. Biovia Discovery Studio was utilized to assess the pharmacokinetic properties of the compounds applying ADMET protocol. Four compounds were isolated and identified from Alternaria destruens NGB-AvF1 including one previously undescribed perylene quinone epoxide ( 1) and three known polyketides ( 2–3 , 4). Metabolic profiling of the extract using UPLC-Q-Tof in the negative mode resulted in the tentative identification of 18 secondary metabolites belonging to different chemical classes with high percentages of polyketides, perylene quinones and isocoumarins. Antimicrobial evaluation revealed strong and selective antimicrobial activities for compounds 2 and 3 against Staphylococcus aureus , with inhibition zones of 28 mm and 30 mm, respectively. In silico assessment revealed favourable binding affinities for 2 and 3 with several key enzymes. Compound 2 exhibited the strongest binding with DNA ligase, β-lactamase, and Clumping factor A, while compound 3 showed high affinity for ClfA, DNA ligase and β-lactamase. Both compounds also interacted moderately with Sortase A, PBP2a, and BlaR1. These results suggest that compounds 2 and 3 possess multitarget antibacterial activity. A. destruens extract revealed wide chemical diversity and displayed a potent antimicrobial activity; in silico studies revealed the potential bacterial targets for the antimicrobial compounds. Compounds 2–3 displayed selective and potent antibacterial activity reinforcing their candidacy as lead compounds for further drug development.",
  "abstract_shown": true,
  "affiliations": [
    "Faculty of Pharmacy, Department of Pharmacognosy, Ain Shams University, Cairo, 11566, Egypt",
    "Department of Microbial Genetic Resources, National Gene Bank (NGB), Agricultural Research Center (ARC), Giza, 12619, Egypt",
    "School of Biotechnology, Nile University, Giza, 12677, Egypt",
    "Faculty of Science, Botany and Microbiology Department, Cairo University, Giza, Egypt",
    "Pharmaceutical and Drug Industries Research Institute, National Research Centre, Giza, Egypt",
    "Microbiology Department, Faculty of Science, Ain Shams University, Cairo, Egypt",
    "Biotechnology and Valorization of Natural Resources Laboratory, Faculty of Sciences, Université Ibn Zohr, Agadir, Morocco",
    "Imbewu Metabolomics Research, Department of Biochemistry, Faculty of Science, University of Johannesburg, Johannesburg, South Africa",
    "Research Centre for Plant Metabolomics, Faculty of Science, University of Johannesburg, Johannesburg, South Africa"
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 12,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Abdel Nasser B. Singab",
      "orcid": "0000-0001-7445-963X"
    },
    {
      "affs": [
        0
      ],
      "name": "Fadia Salah Youssef",
      "orcid": "0000-0002-5871-2639"
    },
    {
      "affs": [
        1
      ],
      "name": "Fayrouz H. Abd El-Megeed"
    },
    {
      "affs": [
        1
      ],
      "name": "Akram H. Mohamed",
      "orcid": "0000-0001-6787-4261"
    },
    {
      "affs": [
        2
      ],
      "name": "Sameh H. Youseif",
      "orcid": "0000-0002-8309-4764"
    },
    {
      "affs": [
        3
      ],
      "name": "Mohamed S. Mahmoud",
      "orcid": "0000-0002-7224-6232"
    },
    {
      "affs": [
        4
      ],
      "name": "Heba A. Dahdooh"
    },
    {
      "affs": [
        5
      ],
      "name": "Naziha Hassanein"
    },
    {
      "affs": [
        6
      ],
      "name": "Mohamed Ben-El-Caid"
    },
    {
      "affs": [
        7
      ],
      "name": "Msizi Innocent Mhlongo",
      "orcid": "0000-0002-6418-6620"
    },
    {
      "affs": [
        8
      ],
      "name": "Anathi Msobo"
    },
    {
      "affs": [
        0
      ],
      "name": "Ahmed M. Elissawy",
      "orcid": "0000-0003-1584-7653"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/fabpgyakc8d7k.bib",
  "cited_by": 5,
  "doi": "10.1186/s43088-026-00793-4",
  "fulltext_url": null,
  "id": "fabpgyakc8d7k",
  "issns": [
    "2314-8535",
    "2314-8543"
  ],
  "landing_url": "https://doi.org/10.1186/s43088-026-00793-4",
  "language": "en",
  "licence": "CC-BY-NC-ND",
  "licence_label": "CC BY-NC-ND",
  "licence_source": "journal",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "2402|Microbial Natural Products and Biosynthesis"
    ]
  ],
  "openalex": "W7169847632",
  "pdf_url": "https://link.springer.com/content/pdf/10.1186/s43088-026-00793-4.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-07-21",
  "publisher": "Springer Science+Business Media",
  "ris": "https://research.draconitic.com/paper/fabpgyakc8d7k.ris",
  "sources": [
    {
      "first_seen": "2026-11-07T13:20:53Z",
      "native_id": "W7169847632",
      "source": "openalex",
      "url": "https://doi.org/10.1186/s43088-026-00793-4"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "openalex:2402|Microbial Natural Products and Biosynthesis",
      "subfield": "microbiology"
    }
  ],
  "title": "UPLC-MS chemical profiling and isolation of bioactive secondary metabolites from Ammi visnaga-derived endophyte Alternaria destruens assessed by in silico studies",
  "title_text": "UPLC-MS chemical profiling and isolation of bioactive secondary metabolites from Ammi visnaga-derived endophyte Alternaria destruens assessed by in silico studies",
  "type": "peer-reviewed",
  "updated_at": "2026-11-08T13:20:46Z",
  "url": "https://research.draconitic.com/paper/fabpgyakc8d7k",
  "venue": "Beni-Suef University Journal of Basic and Applied Sciences",
  "versions": []
}