{
  "abstract": "Amyloid oligomers have been widely implicated as primary cytotoxic intermediates; however, their selective and potentially scalable production remains challenging due to rapid fibril amplification. Here, we demonstrated that sustained axial rotation enables programmable mechanochemical control over amyloid pathway selection without the use of chemical additives. Using a thermal axial rotator, native monomeric hen egg‐white lysozyme was incubated at 60°C under quantitatively tunable rotational speeds, thereby creating RPM‐dependent hydrodynamic boundary conditions through centrifugal and wall‐associated mechanical inputs. A discrete RPM‐dependent transition emerged, delineating a low‐RPM fibril‐amplifying regime, an intermediate transition regime, and a high‐RPM oligomer‐enriched regime. At lower rotational speeds, aggregation followed a fibril‐amplifying pathway characterized by elevated β‐sheet content and elongated fibrillar morphologies. At higher rotational speeds, fibrillar growth was strongly attenuated and oligomer‐enriched assemblies predominated. Spectroscopic analyses and atomic force microscopy revealed that axial rotation redistributes the amyloid assembly landscape rather than simply suppressing aggregation. Functionally, the RPM‐defined assemblies exhibited kinetically distinct seeding behaviors and were associated with differential cellular response profiles in SH‐SY5Y neuroblastoma cells. These findings demonstrate axial rotation–mediated hydrodynamic boundary control as a scalable, chemical‐free strategy for reprogramming amyloid assembly pathways and producing oligomer‐rich assemblies for structural and functional studies.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Biotechnology and Bioinformatics Korea University Sejong Republic of Korea",
    "Interdisciplinary Graduate Program for Artificial Intelligence Smart Convergence Technology Korea University Sejong Republic of Korea",
    "Digital Healthcare Center Sejong Institute for Business and Technology (SIBT) Korea University Sejong Republic of Korea",
    "Department of Digital Healthcare Engineering Korea University Sejong Republic of Korea",
    "Department of Biomedical Engineering Yonsei University Wonju Republic of Korea",
    "Biomedical Research Center Korea University Guro Hospital Seoul Republic of Korea"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 12,
  "authors": [
    {
      "affs": [
        0,
        1
      ],
      "name": "Seokbeom Roh",
      "orcid": "0000-0002-0069-7691"
    },
    {
      "affs": [
        0
      ],
      "name": "Eunji Song",
      "orcid": "0009-0002-4791-6991"
    },
    {
      "affs": [
        0
      ],
      "name": "Minwoo Bae"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Da Yeon Cheong"
    },
    {
      "affs": [
        0
      ],
      "name": "Sechan Han"
    },
    {
      "affs": [
        0,
        2
      ],
      "name": "Taeha Lee"
    },
    {
      "affs": [
        0
      ],
      "name": "Dain Kang"
    },
    {
      "affs": [
        0
      ],
      "name": "Jae Eon Lee"
    },
    {
      "affs": [
        0
      ],
      "name": "Seung Pil Pack",
      "orcid": "0000-0002-4874-0265"
    },
    {
      "affs": [
        2,
        3
      ],
      "name": "Hyungbeen Lee"
    },
    {
      "affs": [
        4
      ],
      "name": "Insu Park",
      "orcid": "0000-0002-3229-8345"
    },
    {
      "affs": [
        0,
        1,
        2,
        3,
        5
      ],
      "name": "Gyudo Lee",
      "orcid": "0000-0001-7895-5112"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/fbz7dz288r23z.bib",
  "cited_by": 0,
  "doi": "10.1002/smll.75467",
  "fulltext_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13725412/",
  "id": "fbz7dz288r23z",
  "issns": [
    "1613-6810",
    "1613-6829"
  ],
  "landing_url": "https://doi.org/10.1002/smll.75467",
  "language": "en",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Research Article"
    ],
    [
      "openalex",
      "2737|Alzheimer's disease research and treatments"
    ]
  ],
  "openalex": "W7162653123",
  "pdf_url": "https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/smll.75467",
  "pmcid": "PMC13725412",
  "pmid": "42638466",
  "published": "2026-08-25",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/fbz7dz288r23z.ris",
  "sources": [
    {
      "first_seen": "2026-11-04T12:23:49Z",
      "native_id": "PMC13725412",
      "source": "pmc",
      "url": "https://doi.org/10.1002/smll.75467"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2737|Alzheimer's disease research and treatments",
      "subfield": "physiology"
    }
  ],
  "title": "Mechanochemically Programmed, Oligomer‐Selective Amyloid Assembly via Axial Rotation",
  "title_text": "Mechanochemically Programmed, Oligomer‐Selective Amyloid Assembly via Axial Rotation",
  "type": "peer-reviewed",
  "updated_at": "2026-11-06T12:21:41Z",
  "url": "https://research.draconitic.com/paper/fbz7dz288r23z",
  "venue": "Small (Weinheim an Der Bergstrasse, Germany)",
  "versions": []
}