{
  "abstract": "Marginal zone (MZ) B cells are hyperresponsive to bacterial Toll-like Receptor (TLR) ligands. However, the full extent of TLR responsiveness for MZ B cells and the mechanisms regulating such responses are unclear. We report that Notch2 activation establishes MZ B cell responsiveness for the viral dsRNA receptor TLR3 and augments responses for the LPS receptor TLR4. Notch2 ligation accelerated Myc induction, mitosis, and plasma cell differentiation to LPS. Further, TLR3 expression in MZ B cells was Notch2 dependent, and ectopic Notch2 signaling was sufficient to promote robust TLR3 responsiveness dependent on the TIR-domain-containing adapter TRIF and the kinase BTK. TLR3 engagement in MZ B cells promoted proliferation, differentiation, and the secretion of IgG2b and IgG2c antibodies. Our results establish a novel role for Notch2 in establishing TLR3 and TLR4 responsiveness in B cells and suggest that MZ B cells play unappreciated roles in immunity against RNA viruses.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia PA 19104-6082, USA.",
    "Department of Inflammation & Immunity, Cleveland Clinic Research, Cleveland, OH, 44195, USA.",
    "Department of Microbiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia PA 19104-6082, USA.",
    "Vaccine and Immunotherapy Center, The Wistar Institute, Philadelphia PA 19104-6082, USA.",
    "Department of Medicine & Human Oncology and Pathogenesis Program, Memorial Sloan Kettering Cancer Center, New York, New York 10065, USA.",
    "Lead contact"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 11,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Jennifer Londregan"
    },
    {
      "affs": [
        0
      ],
      "name": "Isaiah Rozich"
    },
    {
      "affs": [
        1
      ],
      "name": "Brian T. Gaudette"
    },
    {
      "affs": [
        2
      ],
      "name": "Jeffrey R. Maslanka"
    },
    {
      "affs": [
        2
      ],
      "name": "Rachel Serafin"
    },
    {
      "affs": [
        3
      ],
      "name": "Nicholas J. Tursi"
    },
    {
      "affs": [
        0
      ],
      "name": "Sarah Naiyer"
    },
    {
      "affs": [
        2
      ],
      "name": "Scott E. Hensley"
    },
    {
      "affs": [
        3
      ],
      "name": "David B. Weiner"
    },
    {
      "affs": [
        4
      ],
      "name": "Ivan Maillard"
    },
    {
      "affs": [
        0,
        5
      ],
      "name": "David Allman"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/fch8cfcx4rmax.bib",
  "cited_by": 0,
  "doi": "10.1016/j.celrep.2026.117748",
  "fulltext_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13711391/",
  "id": "fch8cfcx4rmax",
  "issns": [
    "2211-1247"
  ],
  "landing_url": "https://doi.org/10.1016/j.celrep.2026.117748",
  "language": "en",
  "licence": "CC-BY-NC",
  "licence_label": "CC BY-NC",
  "licence_source": "journal",
  "licence_url": "https://creativecommons.org/licenses/by-nc/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Article"
    ],
    [
      "openalex",
      "2403|Immune Response and Inflammation"
    ]
  ],
  "openalex": "W4415121242",
  "pdf_url": null,
  "pmcid": "PMC13711391",
  "pmid": "42531127",
  "published": "2026-07-30",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/fch8cfcx4rmax.ris",
  "sources": [
    {
      "first_seen": "2026-10-31T12:22:10Z",
      "native_id": "PMC13711391",
      "source": "pmc",
      "url": "https://doi.org/10.1016/j.celrep.2026.117748"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "openalex:2403|Immune Response and Inflammation",
      "subfield": "immunology"
    }
  ],
  "title": "Notch2 signaling instructs viral and bacterial TLR responsiveness in B cells",
  "title_text": "Notch2 signaling instructs viral and bacterial TLR responsiveness in B cells",
  "type": "peer-reviewed",
  "updated_at": "2026-10-31T12:22:10Z",
  "url": "https://research.draconitic.com/paper/fch8cfcx4rmax",
  "venue": "Cell reports",
  "versions": []
}