{
  "abstract": "Immune cell behavior in tumors can be visualized in real time by imaging ex vivo tumor slices. Here, we present a protocol for preparing mouse tumor slices for ex vivo two-photon microscopy. We describe techniques for adoptive transfer of fluorescent T cells, generation of tumor slices by vibratome, time-lapse two-photon imaging, and quantitative analysis. This protocol provides a model system to study immune cell migration and interactions within solid tumor microenvironments without the technical challenges of intravital microscopy.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Immunology, Mayo Clinic, Phoenix, AZ 85054, USA",
    "Department of Cancer Biology, Mayo Clinic, Phoenix, AZ 85054, USA"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 2,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Tina Kwok"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Jessica N. Lancaster"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/fd5n88nbv6n54.bib",
  "cited_by": 0,
  "doi": "10.1016/j.xpro.2026.104915",
  "fulltext_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13722571/",
  "id": "fd5n88nbv6n54",
  "issns": [
    "2666-1667"
  ],
  "landing_url": "https://doi.org/10.1016/j.xpro.2026.104915",
  "language": "en",
  "licence": "CC-BY-NC-ND-4.0",
  "licence_label": "CC BY-NC-ND 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Protocol"
    ],
    [
      "openalex",
      "1304|Advanced Fluorescence Microscopy Techniques"
    ]
  ],
  "openalex": "W7223466664",
  "pdf_url": null,
  "pmcid": "PMC13722571",
  "pmid": "42925504",
  "published": "2026-10-24",
  "publisher": "Elsevier",
  "ris": "https://research.draconitic.com/paper/fd5n88nbv6n54.ris",
  "sources": [
    {
      "first_seen": "2026-11-04T12:21:22Z",
      "native_id": "PMC13722571",
      "source": "pmc",
      "url": "https://doi.org/10.1016/j.xpro.2026.104915"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1304|Advanced Fluorescence Microscopy Techniques",
      "subfield": "biophysics"
    }
  ],
  "title": "Preparation of mouse tumor slices for ex vivo two-photon microscopy",
  "title_text": "Preparation of mouse tumor slices for ex vivo two-photon microscopy",
  "type": "peer-reviewed",
  "updated_at": "2026-11-04T12:21:22Z",
  "url": "https://research.draconitic.com/paper/fd5n88nbv6n54",
  "venue": "STAR Protocols",
  "versions": []
}