{ "abstract": "Multifunctional wound dressings require polysaccharide-based biomaterials combining structural integrity, antimicrobial activity, and cytocompatibility. In this study, graphene oxide (GO)-functionalized chitosan-carboxymethylcellulose-alginate (ChitCMCAlgGO) sponge scaffolds were developed to investigate the role of GO in modulating the molecular organization and functional properties of a multicomponent polysaccharide network. The composites were characterized using SEM, FT-IR spectroscopy, solid-state ¹³C CP/MAS NMR with T₁ρH and T₁ρC relaxation analyses, mechanical testing, fluid-handling evaluation, antimicrobial and antibiofilm assays, and cytocompatibility assessment according to ISO 10993. GO incorporation enhanced the mechanical performance of the scaffolds, increasing tensile strength from 0.032 to 0.122 MPa and maximum tensile force from 0.93 to 3.48 N, while improving elongation at maximum stress from 37.6% to 110%. Spectroscopic and NMR analyses revealed that GO did not modify the chemical structure of the polysaccharide components but likely promoted non-covalent interactions and altered polymer-chain dynamics, resulting in increased scaffold density and reduced porosity. Despite a moderate decrease in fluid uptake, GO-functionalized scaffolds maintained high absorption capacity (34.2 g g⁻¹ after 24 h). Moreover, GO improved antimicrobial activity against wound-associated pathogens and preserved cytocompatibility. These findings demonstrate that GO functions as a structural nanofiller regulating polysaccharide network organization, providing a rational strategy for designing advanced carbohydrate-based wound dressing materials.", "abstract_shown": true, "affiliations": [ "Pre-clinical Research Center, Wrocław Medical University Karola Marcinkowskiego 1 50-368 Wrocław Poland p.sarelo@umw.edu.pl.", "Department of Biomedical Engineering, Faculty of Fundamental Problems of Technology, Wrocław University of Science and Technology Wybrzeże S. Wyspiańskiego 27 50-370 Wrocław Poland.", "Centre for the Circular Economy, ŁUKASIEWICZ Research Network - Łódź Institute of Technology Marii Skłodowskiej-Curie 19/27 90-570 Łódź Poland.", "Department of Microbiology, Faculty of Medicine, Wrocław Medical University Tytusa Chałubińskiego 4 50-368 Wrocław Poland.", "Division of Optical Spectroscopy, Institute of Low Temperature and Structure Research, Polish Academy of Sciences Okólna 2 50-422 Wrocław Poland.", "Department of Biological, Chemical and Pharmaceutical Sciences and Technologies (STeBiCeF), University of Palermo Viale Delle Scienze, Bld. 16 and 17 90128 Palermo Italy.", "Consiglio Nazionale Delle Ricerche, Istituto per i Processi Chimico Fisici Viale Ferdinando Stagno d'Alcontres 37 98158 Messina Italy.", "Department of Physics and Chemistry \"Emilio Segrè\", University of Palermo Viale Delle Scienze Bld. 17 90128 Palermo Italy." ], "article_type": "research article", "arxiv": null, "author_total": 13, "authors": [ { "affs": [ 0, 1 ], "name": "Przemysław Sareło", "orcid": "0000-0002-9125-7594" }, { "affs": [ 2 ], "name": "Maria Wiśniewska-Wrona", "orcid": "0009-0007-2666-1543" }, { "affs": [ 2 ], "name": "Monika Sikora", "orcid": "0000-0002-0405-7982" }, { "affs": [ 3 ], "name": "Magdalena Pajączkowska", "orcid": "0000-0002-9881-8294" }, { "affs": [ 3 ], "name": "Joanna Nowicka", "orcid": "0000-0002-4001-4189" }, { "affs": [ 4 ], "name": "Yuriy Gerasymchuk", "orcid": "0000-0002-2148-672X" }, { "affs": [ 5, 6 ], "name": "Maria Luisa Saladino", "orcid": "0000-0002-7481-8556" }, { "affs": [ 5 ], "name": "Patrizia Cancemi", "orcid": "0000-0002-3951-7213" }, { "affs": [ 7 ], "name": "Martina Maria Calvino", "orcid": "0000-0002-3417-9032" }, { "affs": [ 7 ], "name": "Giuseppe Lazzara", "orcid": "0000-0003-1953-5817" }, { "affs": [ 0 ], "name": "Maria Szymonowicz", "orcid": "0000-0003-4251-6841" }, { "affs": [ 3 ], "name": "Beata Sobieszczańska", "orcid": "0000-0002-3355-2480" }, { "affs": [ 0 ], "name": "Magdalena Wawrzyńska", "orcid": "0000-0001-6855-5510" } ], "bibtex": "https://research.draconitic.com/paper/fe6yvr011nw2v.bib", "cited_by": 0, "doi": "10.1039/d6ra09378g", "fulltext_url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13717623/?tool=EBI", "id": "fe6yvr011nw2v", "issns": [ "2046-2069" ], "landing_url": "https://europepmc.org/article/PMC/PMC13717623", "language": "eng", "licence": "CC-BY", "licence_label": "CC BY", "licence_source": "article", "licence_url": "https://creativecommons.org/licenses/by/4.0/", "metadata_licence": null, "native_subjects": [ [ "heading", "Chemistry" ], [ "openalex", "2742|Wound Healing and Treatments" ] ], "openalex": "W7224611735", "pdf_url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13717623/pdf/?tool=EBI", "pmcid": "PMC13717623", "pmid": "42959772", "published": "2026-10-29", "publisher": "Royal Society of Chemistry", "ris": "https://research.draconitic.com/paper/fe6yvr011nw2v.ris", "sources": [ { "first_seen": "2026-10-31T00:20:37Z", "native_id": "PMC:PMC13717623", "source": "europepmc", "url": "https://europepmc.org/article/PMC/PMC13717623" }, { "first_seen": "2026-11-01T00:20:50Z", "native_id": "MED:42959772", "source": "europepmc", "url": "https://europepmc.org/article/MED/42959772" }, { "first_seen": "2026-11-02T12:21:54Z", "native_id": "PMC13717623", "source": "pmc", "url": "https://doi.org/10.1039/d6ra09378g" } ], "subjects": [ { "field": "chemistry", "origin": "heading:Chemistry", "subfield": "general" } ], "title": "Structure–property relationships in a graphene oxide-reinforced chitosan–carboxymethylcellulose–alginate composite for wound dressing applications", "title_text": "Structure–property relationships in a graphene oxide-reinforced chitosan–carboxymethylcellulose–alginate composite for wound dressing applications", "type": "peer-reviewed", "updated_at": "2026-11-02T12:21:54Z", "url": "https://research.draconitic.com/paper/fe6yvr011nw2v", "venue": "RSC advances", "versions": [] }