{
  "abstract": "Diabetes mellitus and chronic inflammation are global health issues, exacerbated by the incomplete control of metabolic-inflammatory pathways and the adverse effects of long-term pharmacotherapy. The development of multifunctional therapeutic nanomaterials via green nanotechnology has recently garnered attention. In this study, we synthesized copper-based nanoparticles (Cu NPs) using Salvia hispanica (chia) seed-derived polysaccharides and characterized their in vitro anti-inflammatory and antidiabetic activities, as well as in vivo antidiabetic effects in an alloxan-induced diabetic mouse model. Successful formation of polysaccharide-based Cu NPs was confirmed using UV-Visible, FTIR, XRD, and SEM analyses. Hemocompatibility assessment showed concentration-dependent hemolysis by Cu NPs (0.92 ± 0.06%, 5.71 ± 0.44%, and 8.42 ± 0.63% at the respective tested concentrations), indicating reduced hemocompatibility at higher concentrations. In the HRBC membrane stabilization assay, Cu NPs exhibited dose-dependent inhibition of hemolysis (45.83 ± 1.7% to 70.59 ± 2.1%), while heat hemolysis was reduced by 72.91 ± 1.5% at 1000 µg/mL. Enzyme inhibition studies showed significant inhibition of α-amylase (58.45 ± 0.7% to 87.20 ± 2.1%) and α-glucosidase (83.87 ± 1.6% at 1000 µg/mL) compared to acarbose. Glucose uptake in yeast cells increased to 59.87 ± 0.3% at 1000 µg/mL. In alloxan-induced diabetic mice, Cu NPs (150 mg/kg) reduced fasting blood glucose from 287 ± 4.2 to 131 ± 7.02 mg/dL over 30 days and improved lipid profiles (e.g., decreased total cholesterol to 85 ± 1.3 mg/dL and increased HDL to 39.8 ± 1.2 mg/dL). Histopathological analyses showed restoration of pancreatic and hepatic architecture. Collectively, chia seed polysaccharide-mediated copper-based nanoparticles exhibited anti-hyperglycemic, anti-inflammatory, and lipid-modulating activities under the investigated experimental conditions, supporting their further evaluation for diabetes-related applications.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Biotechnology, Abdul Wali Khan University Mardan, Khyber Pakhtunkhwa, Pakistan.",
    "Faculty of Health and Life Sciences, INTI International University, Nilai, Negeri Sembilan, Malaysia.",
    "Research Chair of Biomedical Applications of Nanomaterials, Biochemistry Department, College of Science, King Saud University, Saudi Arabia.",
    "Botany and Microbiology Department, College of Science, King Saud University, Saudi Arabia.",
    "Department of Food Sciences & Nutrition, College of Food and Agricultural Sciences, King Saud University, Riyadh, Saudi Arabia.",
    "Minnesota Dental Research Center for Biomaterials and Biomechanics, School of Dentistry, University of Minnesota, Minneapolis, Minnesota, United States of America.",
    "Departamento de Ciencias Biológicas y Químicas, Facultad de Recursos Naturales, Universidad Católica de Temuco, Chile.",
    "Núcleo de Estudios Ambientales, Facultad de Recursos Naturales, Universidad Católica de Temuco, Chile.",
    "Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China."
  ],
  "article_type": "Research Article",
  "arxiv": null,
  "author_total": 12,
  "authors": [
    {
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        0
      ],
      "name": "Farzana Khan"
    },
    {
      "affs": [
        0
      ],
      "name": "Yusra Jamil"
    },
    {
      "affs": [
        0
      ],
      "name": "Raeesa Wajid"
    },
    {
      "affs": [
        0
      ],
      "name": "Adnan Khan"
    },
    {
      "affs": [
        0
      ],
      "name": "Muhammad Yaseen"
    },
    {
      "affs": [
        1
      ],
      "name": "Douglas Law"
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    {
      "affs": [
        2
      ],
      "name": "Mostafa A Abdel-Maksoud"
    },
    {
      "affs": [
        3
      ],
      "name": "Saeedah Almutairi"
    },
    {
      "affs": [
        4
      ],
      "name": "Wahidah H Al-Qahtani"
    },
    {
      "affs": [
        5
      ],
      "name": "Waheed Ahmad"
    },
    {
      "affs": [
        6,
        7
      ],
      "name": "Patricio R De Los Ríos-Escalante",
      "orcid": "0000-0001-5056-7003"
    },
    {
      "affs": [
        0,
        8
      ],
      "name": "Ayaz Ahmad",
      "orcid": "0000-0003-3555-4735"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/fenp3sz6spxgk.bib",
  "cited_by": 0,
  "doi": "10.1371/journal.pone.0361692",
  "fulltext_url": "https://europepmc.org/articles/PMC13722162",
  "id": "fenp3sz6spxgk",
  "issns": [
    "1932-6203"
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  "landing_url": "https://doi.org/10.1371/journal.pone.0361692",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Anatomy"
    ],
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      "heading",
      "Animal Cells"
    ],
    [
      "heading",
      "Biochemistry"
    ],
    [
      "heading",
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    ],
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      "heading",
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  "pdf_url": "https://europepmc.org/articles/PMC13722162?pdf=render",
  "pmcid": "PMC13722162",
  "pmid": "42961858",
  "published": "2026-10-30",
  "publisher": "Public Library of Science",
  "ris": "https://research.draconitic.com/paper/fenp3sz6spxgk.ris",
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  "subjects": [
    {
      "field": "biochemistry",
      "origin": "heading:Biochemistry",
      "subfield": "general"
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    {
      "field": "biology",
      "origin": "heading:Cell Biology",
      "subfield": "cell-biology"
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  "title": "Harnessing chia seed polysaccharides for green synthesis of copper-based nanoparticles with antidiabetic and anti-inflammatory activities",
  "title_text": "Harnessing chia seed polysaccharides for green synthesis of copper-based nanoparticles with antidiabetic and anti-inflammatory activities",
  "type": "peer-reviewed",
  "updated_at": "2026-11-03T12:22:43Z",
  "url": "https://research.draconitic.com/paper/fenp3sz6spxgk",
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}