{
  "abstract": "Positive-strand RNA viruses remodel host endomembranes to form replication organelles (ROs). However, the membrane sources for coronavirus double-membrane vesicle (DMV) biogenesis remain unclear. Here, we used proteomic, genetic, pharmacological, and imaging approaches to identify the central membrane supply route for DMV formation. APEX2-based proximity proteomics first identified candidate pathways, followed by functional validation using siRNA knockdown, CRISPR/Cas9 editing, and Golgicide A (GCA) inhibition. DMV formation and viral RNA synthesis were assessed by electron microscopy, immunofluorescence, and qRT-PCR. Golgi-DMV association was analyzed by sucrose gradient fractionation and confocal imaging. In vivo relevance was evaluated in C57BL/6J mice infected with mouse hepatitis virus (MHV) and treated with GCA (intraperitoneal, 50 mg/kg; n = 4-5 per group). We showed that coronaviruses hijack the host GBF1-ARF1-COPI machinery to redirect Golgi-derived membranes to viral replication sites for DMV formation and expansion. Disruption of this pathway markedly impairs viral RNA synthesis, DMV biogenesis, and replication of multiple coronaviruses, including severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Pharmacological inhibition of Golgi-specific Brefeldin A-resistance factor 1 (GBF1) suppressed viral replication and pathology in vivo. Together, our findings reveal a previously unrecognized Golgi-derived membrane supply route for coronavirus ROs and highlight the GBF1-ARF1-COPI pathway as a potential host-directed target for broad-spectrum antiviral therapies.",
  "abstract_shown": true,
  "affiliations": [
    "Centre for Infection and Immunity Study (CIIS), School of Medicine, Shenzhen Campus of Sun Yat-sen University, Guangdong, China.",
    "State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou Medical University, Guangdong, China.",
    "Guangzhou National Laboratory, Guangdong, China.",
    "Key Laboratory of Viral Pathogenesis & Infection Prevention and Control, Jinan University, Ministry of Education, Guangdong, China.",
    "Key Laboratory of Tropical Disease Control of Ministry of Education, Institute of Human Virology, Department of Pathogen Biology and Biosecurity, Zhongshan School of Medicine, Sun Yat-sen University, Guangdong, China."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 13,
  "authors": [
    {
      "affs": [
        0,
        1,
        2,
        3
      ],
      "name": "Zhaohuan Wang"
    },
    {
      "affs": [
        1
      ],
      "name": "Xinyue Su"
    },
    {
      "affs": [
        1
      ],
      "name": "Kaoyi Gong"
    },
    {
      "affs": [
        0
      ],
      "name": "Zixiao Yang"
    },
    {
      "affs": [
        2,
        4
      ],
      "name": "Shu-Rui Liu"
    },
    {
      "affs": [
        2,
        4
      ],
      "name": "Bi-Rong Zheng"
    },
    {
      "affs": [
        1
      ],
      "name": "Keda Shi"
    },
    {
      "affs": [
        0
      ],
      "name": "Yu Ye"
    },
    {
      "affs": [
        1,
        2
      ],
      "name": "Xinrong Zhou"
    },
    {
      "affs": [
        0
      ],
      "name": "Jian Pan"
    },
    {
      "affs": [
        1,
        2
      ],
      "name": "Deyin Guo"
    },
    {
      "affs": [
        1
      ],
      "name": "Panpan Hou"
    },
    {
      "affs": [
        0
      ],
      "name": "Chun-Mei Li"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/fhbaxv6sa0jvq.bib",
  "cited_by": 0,
  "doi": "10.1016/j.hlife.2026.06.004",
  "fulltext_url": "https://europepmc.org/articles/PMC13681541",
  "id": "fhbaxv6sa0jvq",
  "issns": [
    "2949-9283"
  ],
  "landing_url": "https://europepmc.org/article/MED/42903903",
  "language": "eng",
  "licence": "CC-BY-NC-ND",
  "licence_label": "CC BY-NC-ND",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "2725|SARS-CoV-2 and COVID-19 Research"
    ]
  ],
  "openalex": "W7169554131",
  "pdf_url": "https://europepmc.org/articles/PMC13681541?pdf=render",
  "pmcid": "PMC13681541",
  "pmid": "42903903",
  "published": "2026-07-17",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/fhbaxv6sa0jvq.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:19:59Z",
      "native_id": "MED:42903903",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42903903"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2725|SARS-CoV-2 and COVID-19 Research",
      "subfield": "infectious-disease"
    }
  ],
  "title": "Golgi-derived COPI vesicles fuel the biogenesis of coronavirus replication organelles.",
  "title_text": "Golgi-derived COPI vesicles fuel the biogenesis of coronavirus replication organelles.",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:19:59Z",
  "url": "https://research.draconitic.com/paper/fhbaxv6sa0jvq",
  "venue": "HLife",
  "versions": []
}