{
  "abstract": "Background: Transection of the cervical sympathetic trunk (TCST) ameliorates pulmonary arterial hypertension (PAH), but the molecular mechanisms remain unclear. This study aimed to elucidate these mechanisms in monocrotaline (MCT)-induced PAH rats through transcriptomic profiling.\n\nMethods: PAH rats were divided into control, PAH, PAH + sham, and PAH + TCST groups. Therapeutic effects were evaluated by hemodynamics and histology. RNA-seq was performed on lung tissues to identify differentially expressed genes (DEGs), followed by transcription factor (TF) network analysis and alternative splicing analysis, with validation by qPCR and ELISA.\n\nResults: TCST significantly reduced mPAP, RVSP, and RVHI, and attenuated vascular remodeling. RNA-seq identified 500 DEGs (61 upregulated, 439 downregulated). TF network analysis revealed that target genes of differentially expressed TFs were enriched in inflammation, proliferation, and calcium signaling. Multiple PAH-associated genes ( Bmp2 , Ptgs2 , Cxcl13, Wnt7b , Tacr1 , Adra1d ) were downregulated following TCST. Alternative splicing analysis showed that TCST modulated RNA-binding protein (RBP) expression and altered splicing patterns of PAH-relevant genes ( Aldh2 , Il1rn , Foxm1 ). qPCR and ELISA confirmed suppression of the Foxa3 / Adra1d - Tacr1 axis and reduced IP3 production.\n\nConclusions: TCST exerts therapeutic effects through selective reprogramming of TF networks and regulation of RBP-mediated alternative splicing, coordinately suppressing PAH-associated pathways at transcriptional and post-transcriptional levels. These findings provide mechanistic insights and a theoretical basis for developing neuromodulatory strategies for PAH.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Cardiology, General Hospital of Ningxia Medical University",
    "Department of Anesthesiology, General Hospital of Ningxia Medical University"
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 6,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Yu Hao"
    },
    {
      "affs": [
        1
      ],
      "name": "Peipei Li"
    },
    {
      "affs": [
        0
      ],
      "name": "Hua Cheng"
    },
    {
      "affs": [
        0
      ],
      "name": "Ning Yan"
    },
    {
      "affs": [
        0
      ],
      "name": "Hui Zhang"
    },
    {
      "affs": [
        0
      ],
      "name": "Xueping Ma"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/fj3tfmyyrtj5r.bib",
  "cited_by": 0,
  "doi": "10.3389/fcvm.2026.1967847",
  "fulltext_url": null,
  "id": "fj3tfmyyrtj5r",
  "issns": [
    "2297-055X"
  ],
  "landing_url": "https://www.frontiersin.org/articles/10.3389/fcvm.2026.1967847/full",
  "language": null,
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "2740|Pulmonary Hypertension Research and Treatments"
    ]
  ],
  "openalex": "W7224117853",
  "pdf_url": "https://www.frontiersin.org/journals/cardiovascular-medicine/articles/10.3389/fcvm.2026.1967847/pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-28",
  "publisher": "Frontiers Media SA",
  "ris": "https://research.draconitic.com/paper/fj3tfmyyrtj5r.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:20:10Z",
      "native_id": "10.3389/fcvm.2026.1967847",
      "source": "crossref",
      "url": "https://www.frontiersin.org/articles/10.3389/fcvm.2026.1967847/full"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "issn:2297-055X",
      "subfield": "cardiovascular"
    }
  ],
  "title": "Transection of the cervical sympathetic trunk ameliorates pulmonary arterial hypertension by reprogramming transcription factor networks and alternative splicing in rat model",
  "title_text": "Transection of the cervical sympathetic trunk ameliorates pulmonary arterial hypertension by reprogramming transcription factor networks and alternative splicing in rat model",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:20:10Z",
  "url": "https://research.draconitic.com/paper/fj3tfmyyrtj5r",
  "venue": "Frontiers in Cardiovascular Medicine",
  "versions": []
}