{
  "abstract": "Acute radiation syndrome remains a major consequence of high-dose ionizing radiation exposure, and effective pharmacological countermeasures are limited. Nitro-fatty acids possess anti-inflammatory and cytoprotective properties, suggesting potential effectiveness in radiation injury. Here, we investigated the radioprotective effects of 10-nitro-oleic acid (10-NO2OA) in mouse models of total body irradiation (TBI). C57BL/6 mice were exposed to either sublethal (4 Gy) or LD50-level (7.5 Gy) gamma irradiation and treated with 10-NO2OA according to pre-irradiation, post-irradiation, or combined treatment regimens. Survival, hematological responses, bone marrow cells (BMCs)/colony-forming units (CFU), plasma granulocyte colony-stimulating factor (G-CSF), NRF2-responsive signaling, and intestinal responses were evaluated. Administration of 10-NO2OA before and after irradiation significantly improved 30-day survival following 7.5 Gy TBI (57% vs. 20% in vehicle-treated controls). In contrast, post-irradiation treatment alone was ineffective, whereas pre-irradiation treatment alone produced a non-significant trend toward improved survival. The protective effect was absent in BALB/c mice, indicating strain dependence. Although 10-NO2OA did not prevent long-term radiation-induced cytopenia, it increased neutrophil and platelet counts, elevated plasma G-CSF levels shortly after irradiation, and transiently enhanced BMC counts. In irradiated BMCs, 10-NO2OA activated NRF2-responsive gene expression in vitro and in vivo. Exploratory intestinal analyses revealed improved macroscopic appearance and partial restoration of irradiation-suppressed cytokine and growth factor production. In conclusion, 10-NO2OA improves survival following LD50-level gamma irradiation and is associated with modulation of hematopoietic, cytoprotective, and intestinal responses. These findings identify 10-NO2OA as a promising candidate radioprotective agent and support a multifactorial mechanism of action.",
  "abstract_shown": true,
  "affiliations": [
    "Institute of Biophysics of the Czech Academy of Sciences"
  ],
  "article_type": "New results",
  "arxiv": null,
  "author_total": 6,
  "authors": [
    {
      "name": "J. Pereckova"
    },
    {
      "name": "R. Vichova"
    },
    {
      "name": "J. Toufar"
    },
    {
      "name": "D. Chrenko"
    },
    {
      "name": "J. Pospisil"
    },
    {
      "affs": [
        0
      ],
      "name": "T. Perecko"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/fk62pvhtgegyj.bib",
  "cited_by": 0,
  "doi": "10.64898/2026.09.25.754470",
  "fulltext_url": "https://www.biorxiv.org/content/10.64898/2026.09.25.754470v1.full",
  "id": "fk62pvhtgegyj",
  "issns": [],
  "landing_url": "https://www.biorxiv.org/content/10.64898/2026.09.25.754470v1",
  "language": null,
  "licence": "CC-BY-NC-ND",
  "licence_label": "CC BY-NC-ND",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "biorxiv",
      "pharmacology and toxicology"
    ],
    [
      "openalex",
      "2741|Effects of Radiation Exposure"
    ]
  ],
  "openalex": "W7215063731",
  "pdf_url": "https://www.biorxiv.org/content/10.64898/2026.09.25.754470v1.full.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-09-25",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/fk62pvhtgegyj.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:25:40Z",
      "native_id": "10.64898/2026.09.25.754470",
      "source": "biorxiv",
      "url": "https://www.biorxiv.org/content/10.64898/2026.09.25.754470v1"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "biorxiv:pharmacology and toxicology",
      "subfield": "pharmacology-toxicology"
    }
  ],
  "title": "10-Nitro-oleic acid improves survival following gamma irradiation and modulates hematopoietic and intestinal responses",
  "title_text": "10-Nitro-oleic acid improves survival following gamma irradiation and modulates hematopoietic and intestinal responses",
  "type": "preprint",
  "updated_at": "2026-10-30T12:25:40Z",
  "url": "https://research.draconitic.com/paper/fk62pvhtgegyj",
  "venue": "bioRxiv",
  "versions": []
}