{
  "abstract": "Reliable quantification of the biophysical properties of human bile requires analytically validated methods and standardized pre-analytical conditions. We hypothesized that biliary micro-viscosity can be quantified in human bile after liver transplantation (LTx) with predefined analytical performance (accuracy $$\\le 15\\%$$ deviation from reference, reproducibility CV $$<10\\%$$ ). In this method-development and technical-validation study, we established a method to quantify micro-viscosity in human bile after LTx. Validation using sucrose standards demonstrated high accuracy ( $$\\le 15\\%$$ deviation from reference), strong linear correlation ( $$\\textrm{R}^2 = 0.996$$ ), and good reproducibility (CV $$<10\\%$$ ). The method was applied to 21 bile samples from nine patients collected longitudinally after LTx. Mean micro-viscosity was $$1.58 \\pm 0.54~\\text {mPa s}$$ (postoperative day 1), $$1.94 \\pm 0.63~\\text {mPa s}$$ (day 7), and $$1.44 \\pm 0.16~\\text {mPa s}$$ (day 14). Pre-analytical freezing and cooling introduced measurement variability, supporting analysis shortly after collection. These findings demonstrate the analytical performance and practical feasibility of video particle-tracking-based biliary micro-viscosity measurements after LTx. Larger studies combining standardized fresh-sample workflows, biochemical bile characterization, and longitudinal clinical outcome data are required to determine the biological relevance of biliary micro-viscosity and its potential value for future graft modeling.",
  "abstract_shown": true,
  "affiliations": [],
  "article_type": null,
  "arxiv": null,
  "author_total": 12,
  "authors": [
    {
      "name": "Ludwig Lenz"
    },
    {
      "name": "Philip Friedrich"
    },
    {
      "name": "Peggy Stock"
    },
    {
      "name": "Sandra Nickel"
    },
    {
      "name": "Luis Mandl"
    },
    {
      "name": "Anton Schnurpel"
    },
    {
      "name": "Fabian Haak"
    },
    {
      "name": "Uwe Scheuermann"
    },
    {
      "name": "Rebecca Sternkopf"
    },
    {
      "name": "Tim Ricken"
    },
    {
      "name": "Josef Alfons Käs"
    },
    {
      "name": "Hans-Michael Tautenhahn",
      "orcid": "0000-0002-9722-9391"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/fn1gxqvp8fptj.bib",
  "cited_by": 0,
  "doi": "10.1038/s41598-026-74034-0",
  "fulltext_url": null,
  "id": "fn1gxqvp8fptj",
  "issns": [
    "2045-2322"
  ],
  "landing_url": "https://www.nature.com/articles/s41598-026-74034-0",
  "language": null,
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "2746|Organ Transplantation Techniques and Outcomes"
    ]
  ],
  "openalex": "W7221326004",
  "pdf_url": "https://www.nature.com/articles/s41598-026-74034-0.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-10",
  "publisher": "Springer Science and Business Media LLC",
  "ris": "https://research.draconitic.com/paper/fn1gxqvp8fptj.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:17:25Z",
      "native_id": "10.1038/s41598-026-74034-0",
      "source": "crossref",
      "url": "https://www.nature.com/articles/s41598-026-74034-0"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2746|Organ Transplantation Techniques and Outcomes",
      "subfield": "surgery"
    }
  ],
  "title": "Technical validation and feasibility of video particle tracking for low-volume measurement of biliary micro-viscosity after liver transplantation",
  "title_text": "Technical validation and feasibility of video particle tracking for low-volume measurement of biliary micro-viscosity after liver transplantation",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:17:25Z",
  "url": "https://research.draconitic.com/paper/fn1gxqvp8fptj",
  "venue": "Scientific Reports",
  "versions": []
}