{
  "abstract": "Bacterial DNA replication must be tightly controlled to ensure once-per-cell-cycle initiation, requiring precise regulation of the initiator DnaA. The conserved RIDA pathway inactivates DnaA through the Hda–β sliding clamp (DnaN) complex, yet how this system is dynamically regulated has remained unclear. Here, we show that the Hda–DnaN complex exists as a concentration-dependent oligomeric ensemble rather than a static assembly. Using cryo-electron microscopy and mass photometry, we resolve multiple structural states, including an autoinhibited hetero-octamer, a hetero-hexamer intermediate, and a RIDA-compatible hetero-trimer. Structural comparison of these states suggests a plausible route of oligomeric interconversion involving progressive destabilization of Hda self-association, accompanied by exposure of the catalytic arginine finger required for DnaA inactivation. We further propose that this equilibrium operates as a titration-like mechanism that converts local protein concentration into functional states. Together, our findings establish reversible oligomerization as a potential regulatory principle for bacterial replication licensing.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Cell Biology and Anatomy, Graduate School of Medicine, The University of Tokyo , Bunkyo, Tokyo 113-0033 ,",
    "Department of Bioengineering, School of Engineering, The University of Tokyo , Bunkyo, Tokyo 113-8656 ,",
    "Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo , Bunkyo, Tokyo 113-8656 ,",
    "Medical Device Development and Regulation Research Center, School of Engineering, The University of Tokyo , Bunkyo, Tokyo 113-8655 ,",
    "The Institute of Medical Science, The University of Tokyo , Bunkyo, Tokyo 108-8639 ,"
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 6,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Xuguang Jiang",
      "orcid": "0000-0001-7997-872X"
    },
    {
      "affs": [
        0
      ],
      "name": "Radostin Danev",
      "orcid": "0000-0001-6406-8993"
    },
    {
      "affs": [
        0
      ],
      "name": "Yanbin Luan"
    },
    {
      "affs": [
        1,
        2
      ],
      "name": "Makoto Nakakido"
    },
    {
      "affs": [
        1,
        2,
        3,
        4
      ],
      "name": "Kouhei Tsumoto"
    },
    {
      "affs": [
        0
      ],
      "name": "Masahide Kikkawa",
      "orcid": "0000-0001-7656-8194"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/fnk2cksevc57d.bib",
  "cited_by": 0,
  "doi": "10.1093/nar/gkag1010",
  "fulltext_url": null,
  "id": "fnk2cksevc57d",
  "issns": [
    "0305-1048",
    "1362-4962"
  ],
  "landing_url": "https://academic.oup.com/nar/article/doi/10.1093/nar/gkag1010/8986691",
  "language": null,
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1311|Bacterial Genetics and Biotechnology"
    ]
  ],
  "openalex": "W7223321045",
  "pdf_url": "https://academic.oup.com/nar/article-pdf/54/19/gkag1010/72410973/gkag1010.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-23",
  "publisher": "Oxford University Press (OUP)",
  "ris": "https://research.draconitic.com/paper/fnk2cksevc57d.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:18:50Z",
      "native_id": "10.1093/nar/gkag1010",
      "source": "crossref",
      "url": "https://academic.oup.com/nar/article/doi/10.1093/nar/gkag1010/8986691"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "openalex:1311|Bacterial Genetics and Biotechnology",
      "subfield": "genetics-genomics"
    }
  ],
  "title": "Reversible autoinhibition of the bacterial replication-licensing Hda–β clamp complex",
  "title_text": "Reversible autoinhibition of the bacterial replication-licensing Hda–β clamp complex",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:18:50Z",
  "url": "https://research.draconitic.com/paper/fnk2cksevc57d",
  "venue": "Nucleic Acids Research",
  "versions": []
}