{
  "abstract": "Background\n\nAtopic dermatitis (AD) is a chronic inflammatory skin disorder with limited therapeutic options.Objective\n\nThis study evaluated the therapeutic potential of amygdalin by identifying urokinase-type plasminogen activator (uPA/PLAU)-associated molecular targets and immune-inflammatory signaling pathways, aiming to explore a potential strategy for natural product-based interventions.Methods\n\nTranscriptomics, network pharmacology, and molecular docking were integrated to predict potential targets of amygdalin and intersect them with AD-related differentially expressed genes to identify key candidate molecules. A 2,4-dinitrochlorobenzene-induced BALB/c mouse model was used to evaluate therapeutic effects through histopathology, cytokine profiling, quantitative real-time polymerase chain reaction (qRT-PCR), and Western blot analyses of PLAU/uPA-associated signaling, including the NLR family pyrin domain containing 3 (NLRP3) inflammasome and Toll-like receptor 4 (TLR4)/MyD88/nuclear factor (NF)-κB pathways.Results\n\nPLAU was identified as a key candidate target potentially involved in the action of amygdalin. In vivo, amygdalin significantly reduced dermatitis severity and ear thickness (p<0.001), improved histopathology, and lowered serum immunoglobulin E, histamine, and thymus- and activation-regulated chemokine as well as skin interleukin (IL)-6, IL-1β, and tumor necrosis factor-α levels (p<0.001). It also partially corrected Th1/Th2 imbalance by increasing interferon-γ and decreasing IL-4 and IL-13 (p<0.001). qRT-PCR and Western blot showed reduced expression of PLAU/uPA, 3-hydroxy-3-methylglutaryl-coenzyme A reductase, and cathepsin K, together with reduced activation of the NLRP3 inflammasome and TLR4/MyD88/NF-κB signaling.Conclusion\n\nAmygdalin exhibited anti-inflammatory and immunoregulatory effects in experimental AD and was associated with modulation of PLAU/uPA-related immune-inflammatory pathways, suggesting potential as a multi-target natural therapeutic candidate. However, the present study did not directly quantify or fully characterize the cyanogenic risk of amygdalin, and its safety profile-particularly regarding cyanide generation-requires further evaluation in dedicated toxicological investigations.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Dermatology, Affiliated Hospital of Jiangnan University, Wuxi, China. zjyfjfbfy2025@163.com.",
    "Department of Dermatology, Affiliated Hospital of Jiangnan University, Wuxi, China."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 3,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Jiayin Zhang",
      "orcid": "0009-0004-6246-1088"
    },
    {
      "affs": [
        1
      ],
      "name": "Mi Zhong",
      "orcid": "0009-0009-1488-6424"
    },
    {
      "affs": [
        1
      ],
      "name": "Fen Zeng",
      "orcid": "0009-0003-2698-7958"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/fnsbntesrf942.bib",
  "cited_by": 0,
  "doi": "10.5021/ad.25.201",
  "fulltext_url": "https://europepmc.org/articles/PMC13655623",
  "id": "fnsbntesrf942",
  "issns": [
    "1013-9087",
    "2005-3894"
  ],
  "landing_url": "https://europepmc.org/article/MED/42845201",
  "language": "eng",
  "licence": "CC-BY-NC",
  "licence_label": "CC BY-NC",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "2708|Dermatology and Skin Diseases"
    ]
  ],
  "openalex": "W7203611946",
  "pdf_url": "https://europepmc.org/articles/PMC13655623?pdf=render",
  "pmcid": "PMC13655623",
  "pmid": "42845201",
  "published": "2026-10-01",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/fnsbntesrf942.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:18:10Z",
      "native_id": "MED:42845201",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42845201"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2708|Dermatology and Skin Diseases",
      "subfield": "general"
    }
  ],
  "title": "Amygdalin Targets PLAU to Modulate Immune-Inflammatory Signaling and Ameliorate Atopic Dermatitis: Evidence From Multi-Omics and an Animal Model.",
  "title_text": "Amygdalin Targets PLAU to Modulate Immune-Inflammatory Signaling and Ameliorate Atopic Dermatitis: Evidence From Multi-Omics and an Animal Model.",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:18:10Z",
  "url": "https://research.draconitic.com/paper/fnsbntesrf942",
  "venue": "Annals of dermatology",
  "versions": []
}