{
  "abstract": "Scale drop disease virus (SDDV) and Vibrio harveyi are important infectious agents affecting farmed Asian seabass (Lates calcarifer). Fish infected with these pathogens usually exhibit similar clinical signs, making differential diagnosis challenging. This study aimed to develop a duplex PCR protocol for the simultaneous detection of both pathogens in farmed Asian seabass. The newly developed method demonstrated high sensitivity (20 copies per reaction) and high specificity with no cross-amplification observed. It successfully detected infections in clinical samples (n = 42) and revealed co-infections in 11/42 samples. This duplex PCR protocol offers a useful molecular tool for detecting individual or concurrent infections, thereby contributing to enhanced disease diagnosis, surveillance and monitoring in Asian seabass aquaculture. • Simultaneous detection of SDDV and V. harveyi in a single PCR reaction • High sensitivity and specificity without cross-reactivity • Applicable to clinical samples for confirmatory disease diagnosis.",
  "abstract_shown": true,
  "affiliations": [
    "Fish Health Platform, Center of Excellence for Shrimp Molecular Biology and Biotechnology (Centex Shrimp), Faculty of Science, Mahidol University, Bangkok, 10400, Thailand",
    "Faculty of Marine Technology, Burapha University Chanthaburi Campus, Chanthaburi, 22170, Thailand",
    "Aquaculture and Aquatic Resources Management Program, Faculty of Food, Agriculture, and Natural Resources, Asian Institute of Technology, 12120, Thailand"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 7,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Watcharachai Meemetta"
    },
    {
      "affs": [
        0
      ],
      "name": "Suwimon Taengphu"
    },
    {
      "affs": [
        0
      ],
      "name": "Onanong Charoenwai"
    },
    {
      "affs": [
        1
      ],
      "name": "Sakulrat Kongmuang"
    },
    {
      "affs": [
        1
      ],
      "name": "Molruedee Sonthi"
    },
    {
      "affs": [
        2
      ],
      "name": "Ha Thanh Dong"
    },
    {
      "affs": [
        0
      ],
      "name": "Saengchan Senapin"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/fp667dy26j640.bib",
  "cited_by": 0,
  "doi": "10.1016/j.mex.2026.104208",
  "fulltext_url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13718649/?tool=EBI",
  "id": "fp667dy26j640",
  "issns": [
    "2215-0161"
  ],
  "landing_url": "https://europepmc.org/article/PMC/PMC13718649",
  "language": "eng",
  "licence": "CC-BY-NC-ND",
  "licence_label": "CC BY-NC-ND",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Biochemistry, Genetics and Molecular Biology"
    ],
    [
      "openalex",
      "2403|Aquaculture disease management and microbiota"
    ]
  ],
  "openalex": "W7221665242",
  "pdf_url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13718649/pdf/?tool=EBI",
  "pmcid": "PMC13718649",
  "pmid": "42959901",
  "published": "2026-10-13",
  "publisher": "Elsevier",
  "ris": "https://research.draconitic.com/paper/fp667dy26j640.ris",
  "sources": [
    {
      "first_seen": "2026-10-31T00:20:38Z",
      "native_id": "PMC:PMC13718649",
      "source": "europepmc",
      "url": "https://europepmc.org/article/PMC/PMC13718649"
    },
    {
      "first_seen": "2026-10-31T12:21:46Z",
      "native_id": "MED:42959901",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42959901"
    },
    {
      "first_seen": "2026-11-02T12:22:38Z",
      "native_id": "PMC13718649",
      "source": "pmc",
      "url": "https://doi.org/10.1016/j.mex.2026.104208"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "openalex:2403|Aquaculture disease management and microbiota",
      "subfield": "immunology"
    }
  ],
  "title": "Duplex PCR method for simultaneous detection of scale drop disease virus and Vibrio harveyi in Asian seabass (Lates calcarifer)",
  "title_text": "Duplex PCR method for simultaneous detection of scale drop disease virus and Vibrio harveyi in Asian seabass (Lates calcarifer)",
  "type": "peer-reviewed",
  "updated_at": "2026-11-02T12:22:38Z",
  "url": "https://research.draconitic.com/paper/fp667dy26j640",
  "venue": "MethodsX",
  "versions": []
}